DHFR dihydrofolate: Difference between revisions
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MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFNYFQRMTKTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKESPQGAHFLAKSLDDALKLIEEPELANKVDMVWIVGGSSVYKEAMNQPGHVKLFVTRIMQDFECDTFFPEIDLKKYKLLSEYPGVLSDVQEEKGIKYKFEVYEKND | MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFNYFQRMTKTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKESPQGAHFLAKSLDDALKLIEEPELANKVDMVWIVGGSSVYKEAMNQPGHVKLFVTRIMQDFECDTFFPEIDLKKYKLLSEYPGVLSDVQEEKGIKYKFEVYEKND | ||
> | >DHFR_micMur Microcebus murinus (lemur) wgs Blat | ||
MVRTLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQKMTTTSSVEGKQNLVIMGRKTWFSIPEKSRPLKDRINVVLSRELKEPPQGAHFVAKSLDDALKLIEQPELANKVDMVWIVGGSAVYKEAMNQPGHLKLFVTRIMQDFESDTFFPEIDLKKYKLLPE YPGVLSDVQEEKGIKYKFEVYEKND | MVRTLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQKMTTTSSVEGKQNLVIMGRKTWFSIPEKSRPLKDRINVVLSRELKEPPQGAHFVAKSLDDALKLIEQPELANKVDMVWIVGGSAVYKEAMNQPGHLKLFVTRIMQDFESDTFFPEIDLKKYKLLPE YPGVLSDVQEEKGIKYKFEVYEKND | ||
Line 266: | Line 266: | ||
MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVEGKQNIVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLAKSLDDALKLIEQPELANQVDMVWIVGGSSVYREAMNQPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPE | MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVEGKQNIVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLAKSLDDALKLIEQPELANQVDMVWIVGGSSVYREAMNQPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPE | ||
>DHFR_musMus Mus musculus (mouse) | >DHFR_musMus Mus musculus (mouse) NP_034179 F32W is methotrexate resistant | ||
MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQPELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEYPGVLSEVQEEKGIKYKFEVYEKKD | |||
>DHFR_ratNor Rattus norvegicus (rat) NM_130400 | >DHFR_ratNor Rattus norvegicus (rat) NM_130400 | ||
Line 316: | Line 316: | ||
>DHFR_equCab Equus caballus (horse) XM_001504643 | >DHFR_equCab Equus caballus (horse) XM_001504643 | ||
MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTASSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLAKSLDDALSLLEQPELANKVDMVWIVGGSSVYKEAMNKPGHLRLFVTRIMQEFESDTFFPEIDFEKYKLLPEYPGVLSDIQEEKGIKYKFEVYEKND | MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTASSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLAKSLDDALSLLEQPELANKVDMVWIVGGSSVYKEAMNKPGHLRLFVTRIMQEFESDTFFPEIDFEKYKLLPEYPGVLSDIQEEKGIKYKFEVYEKND | ||
>DHFR_cerSim Ceratotherium simum (rhino) AKZM01005342 | |||
MMRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLAKSLEDALSLIEQPELTNKVDMVWIVGGSSVYKEAMNKPGHLRLFVTRIMQEFESDTFFPEIDFKKYKLLPEYPGVLSDIQEEKGIKYKFEVYEKND | |||
>DHFR_vicPac Vicugna paca (vicuna) wgsBlast | >DHFR_vicPac Vicugna paca (vicuna) wgsBlast | ||
Line 340: | Line 343: | ||
>DHFR_pteVam Pteropus vampyrus (macrobat) wgsBlast | >DHFR_pteVam Pteropus vampyrus (macrobat) wgsBlast | ||
MVLSLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSveGKRNLVIMGRKTWFSIPKKNRPLKDRINLVLSRELKEPPKGAHFLAKSLDDALKLIEQPELRSKVDMVWIVGGSSVYKEAMNKPGHIKLFVTKIMQEFESDIFFPEIDLGKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND | MVLSLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSveGKRNLVIMGRKTWFSIPKKNRPLKDRINLVLSRELKEPPKGAHFLAKSLDDALKLIEQPELRSKVDMVWIVGGSSVYKEAMNKPGHIKLFVTKIMQEFESDIFFPEIDLGKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND | ||
>DHFR_eptFus Eptesicus fuscus (brown bat) ALEH01016635 ALEH01087690 ALEH01016633 | |||
MVRTLNCIAAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTTSAEGKQNLVIMGRKTWFSIPEKSRPLKDRINLVLSRELKEPPKGAHFLAKSLDDALKLIEEPELTNKVDMVWIVGGSSVYKEAMSKPGHQRLFVTRIMQEFESDTFFPEIDTEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKNE | |||
>DHFR_eriEur Erinaceus europaeus (hedgehog) wgsBlast/ti|909141827 | >DHFR_eriEur Erinaceus europaeus (hedgehog) wgsBlast/ti|909141827 | ||
Line 346: | Line 352: | ||
>DHFR_sorAra Sorex araneus (shrew) wgsBlast | >DHFR_sorAra Sorex araneus (shrew) wgsBlast | ||
MVRPLNCIVAVSQNMGIGKNGELPWPPLRNEFTYFRKMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPTGAHFLAKSLDDALKLIEQPELTNKVDMVWIVGGSSVYQEVMNKPGHLRLFVTRIMQEFECDTFFPEIDLEKYKLLTDYPGVVSDVQEEKGIKYKFEVYEKKD | MVRPLNCIVAVSQNMGIGKNGELPWPPLRNEFTYFRKMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPTGAHFLAKSLDDALKLIEQPELTNKVDMVWIVGGSSVYQEVMNKPGHLRLFVTRIMQEFECDTFFPEIDLEKYKLLTDYPGVVSDVQEEKGIKYKFEVYEKKD | ||
>DHFR_eriEur Erinaceus europaeus (hedgehog) AMDU01007281 AMDU01007279 AANN01114614 AANN01744600 AMDU01007278 | |||
MVRPLNCMVAVSQNMGIGKNGELPWPPLRNEFKHFRTMTSTPSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLAKSLDDALKLIDQPELTNKVDRVWIIGGSSVYKEVMNRPGHIRLFVTRILQEFECDTFFPEIDLKNYRLLPEYPGVLSDIQEEKGIKYKFEVYERND | |||
>DHFR_conCri Condylura cristata (starnosed mole) AJFV01021063 | |||
MVRPLNCIVAVSQNMGIGKNGDLPWPSLRNEYSYFRRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINVVLSRELKEPPQGAHFLAKSLDDALKLIEQPELTNKVDMVWIVGGSSVYKEAINKSGHIRLFVTRIMQEFESDTFFPEIDLKKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND | |||
>DHFR_loxAfr Loxodonta africana (elephant) XM_003408117 error single trace TTTTTSSV | >DHFR_loxAfr Loxodonta africana (elephant) XM_003408117 error single trace TTTTTSSV | ||
MVRPLNCIVAVSQNMGIGKNGDLPwPplRNEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRGLKEPPQGAHFLAKSLDDALKLIEQPELRNKVDMVWIVGGSSVYKEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLEKYKRLSEYPGVLSDVQEEKGIKYKFEVYEKKD | |||
>DHFR_triMan Trichechus manatus (manatee) AHIN01038459 AHIN01038460 | |||
MVRRLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTTTSSEEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLAKSLDDALKLIEQPELENKVDMVWIVGGSSVYKEAMNHPGHLRLFVTRIMQEFESDTFFPEIDFKKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND | |||
>DHFR_proCap Procavia capensis (hyrax) wgsBlast | >DHFR_proCap Procavia capensis (hyrax) wgsBlast | ||
MVRPLSCIVAVSQNMGIGKNGDLPWPPLRNEFKHFRTMTSTPSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLAKSLDDALKLIEQPELTNKVDLVWIIGGSSVYKEAMSQPGHLTLFVTRIMQEFESDTFFPEIDLEKFKLLPEYPGVPSDVQEEKGIKYKFEVYEKKD | MVRPLSCIVAVSQNMGIGKNGDLPWPPLRNEFKHFRTMTSTPSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLAKSLDDALKLIEQPELTNKVDLVWIIGGSSVYKEAMSQPGHLTLFVTRIMQEFESDTFFPEIDLEKFKLLPEYPGVPSDVQEEKGIKYKFEVYEKKD | ||
> | >DHFR_eleEdw Elephantulus edwardii (elephant shrew) AMGZ01128973 AMGZ01128972 AMGZ01128971 | ||
MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPQGAHFLAKSLDEALKLIEQPELRNKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLQKYKLLPKYPGVASDIQEEKGIKYKFEVYEKKD | |||
>DHFR_choHof Choloepus hoffmanni (sloth) | >DHFR_chrAsi Chrysochloris asiatica (golden mole) AMDV01216092 AMDV01216090 AMDV01216089 | ||
MVRPINCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHLLARSLDDALKLIEQPELGNKVDIVWIVGGSSVYKDAMNQPGHVRLFVTRIMQEFESDTFFPEIDLEKYRLLPEYPGVLSAVQEEKGIKYKFEVYEKND | |||
>DHFR_oryAfe Orycteropus afer (aardvark) ALYB01132696 ALYB01132694 ALYB01132692 | |||
MVRQLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTTSSSVDGKQNVVIMGRKTWFSIPKKHRPLKDRINLVLSRELKEPPQGAHFLAKSLDDALKLIEQPEIGNKVEMVWIIGGSSVYKEAMDQPGHLRLFVTRIMQEFESDTFFPEIDLQKYKLLPEYPGVLSDVQEENGIKYKFEVYEKNE | |||
>DHFR_dasNov Dasypus novemcinctus (armadillo) AAGV03076267 AAGV03076265 | |||
MVRPLNCIVAVSQNMGIGKNGDMPWPPLRNEYKYFQKMTTVSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELREPPQGAHFLAKSLDDALKLIEQPELADKVDMIWIVGGSSVYKEAMNQPGHIRLFVTRIMQEFECDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND | |||
>DHFR_choHof Choloepus hoffmanni (sloth) ABVD01033446 ABVD01033450 | |||
MVRPLNCIVAVSQNMGIGKNGDLPWPLLRNEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLAKSLDDALKLIEQPELANKVDMVWIVGGSSVYKEAMNRPGHLRLFVTRIMQEFESDTFFPEIDTEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND | |||
>DHFR_monDom Monodelphis domestica (opossum) XM_001363581 | >DHFR_monDom Monodelphis domestica (opossum) XM_001363581 | ||
Line 429: | Line 453: | ||
>DHFR_lepOcu Lepisosteus oculatus (spotted_gar) AHAT01012362 | >DHFR_lepOcu Lepisosteus oculatus (spotted_gar) AHAT01012362 | ||
0 | 0 MPRPINCIVAVCPNMGIGHNGNLPWHPKRLS 2 | ||
1 NEFKYFQKMTMTPTLE 1 | 1 NEFKYFQKMTMTPTLE 1 | ||
2 GQQNAVIMGRKTWFSIPERNRPLKNRINIVLSRELK 2 | 2 GQQNAVIMGRKTWFSIPERNRPLKNRINIVLSRELK 2 | ||
Line 478: | Line 502: | ||
MSRVLNCIVAVCPDMGIGNNGNLPWHPKRLNNEFKYFQKMTMTSSVEGKQNAVIMGRKTWFSIPERNRPLKNRINIVLSRELKEPPAGAHQLASDFRSALRLLDTAEMAERVDQVWVIGGSSLYEEMMESQGTRRLFVTRILQQFKSDTFLPEINQDKYRLLPEFPGVPQDLQEEAGLQYRFEVYESIDQ | MSRVLNCIVAVCPDMGIGNNGNLPWHPKRLNNEFKYFQKMTMTSSVEGKQNAVIMGRKTWFSIPERNRPLKNRINIVLSRELKEPPAGAHQLASDFRSALRLLDTAEMAERVDQVWVIGGSSLYEEMMESQGTRRLFVTRILQQFKSDTFLPEINQDKYRLLPEFPGVPQDLQEEAGLQYRFEVYESIDQ | ||
>DHFR_danRer Danio rerio (zebrafish) NM_131775 | >DHFR_danRer Danio rerio (zebrafish) NM_131775 genome duplicate BC165261 | ||
MSRILNCIVAVCPDMGIGKNGNLPWHPIRLSNELKHFQKMTMTPSDEGKKNVVIMGRKTWFSIPAAHRPLKNRINIVLSRELKTAPEGAHYLASDFSSALHLLDSGELEKLVDQVWIIGGSSLYKEVMERSGHRRLFVTRILKQFDCDTFIPNFDMDKYKLLPEFPGVPVGLQEDNGVQYLFEVYESIKH | MSRILNCIVAVCPDMGIGKNGNLPWHPIRLSNELKHFQKMTMTPSDEGKKNVVIMGRKTWFSIPAAHRPLKNRINIVLSRELKTAPEGAHYLASDFSSALHLLDSGELEKLVDQVWIIGGSSLYKEVMERSGHRRLFVTRILKQFDCDTFIPNFDMDKYKLLPEFPGVPVGLQEDNGVQYLFEVYESIKH | ||
Line 495: | Line 519: | ||
>DHFR_squAca Squalus acanthias (dogfish) DV497998 | >DHFR_squAca Squalus acanthias (dogfish) DV497998 | ||
mPRLVNCIVAVCPNMGIGKDGNFPWHPIRLSKELKHFQKMTATPSVEGKRNAVIMGRKTWFSIPEKHRPLKDRLNIVLSRELKELPHGANYLAHDLDSALAHLDSSELQDKVDLVWIIGGSTLYKEAMESPVSHRIFVTRILKDFESDTFLPEMDLNKFKLLPKFPDVPTEVQEDNGIQYKFEVYERIAHA | mPRLVNCIVAVCPNMGIGKDGNFPWHPIRLSKELKHFQKMTATPSVEGKRNAVIMGRKTWFSIPEKHRPLKDRLNIVLSRELKELPHGANYLAHDLDSALAHLDSSELQDKVDLVWIIGGSTLYKEAMESPVSHRIFVTRILKDFESDTFLPEMDLNKFKLLPKFPDVPTEVQEDNGIQYKFEVYERIAHA | ||
>DHFR_calMil calMil Callorhinchus milii (elephant shark) AAVX01229006 fragment | |||
0 MPRMLNSIVAVCPNMGIGKDGNLPWHPKRLN 2 | |||
1 KEFKHFQRMTSTPS 1 | |||
>DHFR_eptBur Eptatretus burgeri (hagfish) BJ646201 | >DHFR_eptBur Eptatretus burgeri (hagfish) BJ646201 | ||
Line 763: | Line 791: | ||
MGPKLYIIAAACENMGIGYKNDLPWKLRNEMAYFNRMTQIVENPGSGSELKNAVIMGRRTWDSIPEKFRPLRNRLNIVLTSRPDFKQPNVLSYPGWYEAVKDLATPEKSLQIDKVWVIGGNSTYKLAVESPDCHRIYLTRVLKHFECDAFFPEFDKSKFRLANDPNVPQDIQEEDGIQYHFEI | MGPKLYIIAAACENMGIGYKNDLPWKLRNEMAYFNRMTQIVENPGSGSELKNAVIMGRRTWDSIPEKFRPLRNRLNIVLTSRPDFKQPNVLSYPGWYEAVKDLATPEKSLQIDKVWVIGGNSTYKLAVESPDCHRIYLTRVLKHFECDAFFPEFDKSKFRLANDPNVPQDIQEEDGIQYHFEI | ||
> | >DHFR_calCle Caligus clemensi (sea_louse) BT080999 Crustacea; Maxillopoda; Copepoda | ||
MTSLKCVKINVIAAACKSSRGIGKDNDLPWKLPTDMKYFREKTASTTEIRVGVIMGRRTWESVPPKFRPFKNRFNVVLTSREDFEAKGAVIARTLEDAVKKCSNNVDSIWVIGGSGVYAEALNKYPCRVYLTEIDKEFDCDAFFPPLDEKKYKLASVSPDHQENDITFRFKVYDVIEL | MTSLKCVKINVIAAACKSSRGIGKDNDLPWKLPTDMKYFREKTASTTEIRVGVIMGRRTWESVPPKFRPFKNRFNVVLTSREDFEAKGAVIARTLEDAVKKCSNNVDSIWVIGGSGVYAEALNKYPCRVYLTEIDKEFDCDAFFPPLDEKKYKLASVSPDHQENDITFRFKVYDVIEL | ||
Line 974: | Line 1,002: | ||
MLALVVALASHRGIGNANALPWPRPLAADMAWFRTLSQSIPLISPDRIALAPSASNAVVMGRRTWDSIPSRFRPLANRINVVLSRGPARSTENTFFIQTFEALDSLPLPPSSMTFVIGGRDVYSLALQSGRPHLIFATEVFESPECDVFFPHIDWASYEKRDITRDVSRLIDRTLASAFYSPETATFTENGTSFKMFLYTKPETR | MLALVVALASHRGIGNANALPWPRPLAADMAWFRTLSQSIPLISPDRIALAPSASNAVVMGRRTWDSIPSRFRPLANRINVVLSRGPARSTENTFFIQTFEALDSLPLPPSSMTFVIGGRDVYSLALQSGRPHLIFATEVFESPECDVFFPHIDWASYEKRDITRDVSRLIDRTLASAFYSPETATFTENGTSFKMFLYTKPETR | ||
> | >DHFR_encInt Encephalitozoon intestinalis (microsporidia) NC_014415 tandem DHFR-TS | ||
MIALIVALAANQGIGRHGKLPWKHPLKTDMAWFRILSQSVPILSPDHISLSPSKSNVVVMGRKTWHSIPSRFRPLQNRINVVLSRSSLPHSQNTFFIPSFAALDHLPLPPSPATFVIGGHDIYALAIQTGRVQAMFVTEVHESPECDVFFPQVDWSSYQKRDITRDVARLVDTTLVDAFYIPEENIFNEGGISFKMFIYTKLHP | MIALIVALAANQGIGRHGKLPWKHPLKTDMAWFRILSQSVPILSPDHISLSPSKSNVVVMGRKTWHSIPSRFRPLQNRINVVLSRSSLPHSQNTFFIPSFAALDHLPLPPSPATFVIGGHDIYALAIQTGRVQAMFVTEVHESPECDVFFPQVDWSSYQKRDITRDVARLVDTTLVDAFYIPEENIFNEGGISFKMFIYTKLHP | ||
Line 1,024: | Line 1,052: | ||
>DHFR_ostTau Ostreococcus tauri (green alga) XP_003074642 Viridiplantae; Chlorophyta trifunctional dehydrogenase-DHFR-TS smallest free-living eukaryote | >DHFR_ostTau Ostreococcus tauri (green alga) XP_003074642 Viridiplantae; Chlorophyta trifunctional dehydrogenase-DHFR-TS smallest free-living eukaryote | ||
MASYYRALAFALAGARRYGRDGYEAADLEPLREVQNLVGTAFVVTGGSRGIGFAVSEGLIRRGARVHVVCRDEATGASARERLNALGTRTGGSCEVHVSDLSSLAQTRGLVEKLASRGEALHGLVCNAGVMRRERTTSSEGYExxxxxxxDGASRGPQKGPRVTPDASRGLILKSAPAELDGDAVPTSTSTPGRATSSARRLAWGLARGIDALGAFARAGGRSSTGGGGSATRKVRCASARAIGEAKRSRGG | MASYYRALAFALAGARRYGRDGYEAADLEPLREVQNLVGTAFVVTGGSRGIGFAVSEGLIRRGARVHVVCRDEATGASARERLNALGTRTGGSCEVHVSDLSSLAQTRGLVEKLASRGEALHGLVCNAGVMRRERTTSSEGYExxxxxxxDGASRGPQKGPRVTPDASRGLILKSAPAELDGDAVPTSTSTPGRATSSARRLAWGLARGIDALGAFARAGGRSSTGGGGSATRKVRCASARAIGEAKRSRGG | ||
MEMRAFQVVVAATVDGGIGKDNGLPWKLTKDMGHFKKLTSETRDANAVNAVVMGRKTWESIPAKFRPLPGRLNVVLSRSGVLAEANDENNNGAETLPEGVLVRKTIDDALSAISSSEKRVEKTFVIGGAQIYEEALKSERCEAVHLTEVEGDFECDVFIPKIDADKFKLYGQSKPITENGARYQFLTYVTKDSESGKFRPIAGELLPAGCDVK | MEMRAFQVVVAATVDGGIGKDNGLPWKLTKDMGHFKKLTSETRDANAVNAVVMGRKTWESIPAKFRPLPGRLNVVLSRSGVLAEANDENNNGAETLPEGVLVRKTIDDALSAISSSEKRVEKTFVIGGAQIYEEALKSERCEAVHLTEVEGDFECDVFIPKIDADKFKLYGQSKPITENGARYQFLTYVTKDSESGKFRPIAGELLPAGCDVK | ||
HEEHQYLDMIREIIDQGAVKGDRTGTGTISTFGNQMRFDLRRSFPLLTTKRVFWRGVAEELLWFVAGETNANKLAEKKINIWDGNGSREYLDSIGLTEREVGDLGPVYGFQWRHFGAEYTNMHADYTGKGVDQLAEVINKIKNNPTDRRILLTAWNPAALKEMALPPCHMFCQFYVANGELSCQMYQRSCDMGLGVPFNIASYSLLTCMIAQVCDLKPGDFVHVCGDTHVYSNHVEPLKKQLTCEPRPFPILKINPEKKDIDSFTFDDFEIIGYDPHPKIEMKMAV | HEEHQYLDMIREIIDQGAVKGDRTGTGTISTFGNQMRFDLRRSFPLLTTKRVFWRGVAEELLWFVAGETNANKLAEKKINIWDGNGSREYLDSIGLTEREVGDLGPVYGFQWRHFGAEYTNMHADYTGKGVDQLAEVINKIKNNPTDRRILLTAWNPAALKEMALPPCHMFCQFYVANGELSCQMYQRSCDMGLGVPFNIASYSLLTCMIAQVCDLKPGDFVHVCGDTHVYSNHVEPLKKQLTCEPRPFPILKINPEKKDIDSFTFDDFEIIGYDPHPKIEMKMAV | ||
Line 1,174: | Line 1,202: | ||
>DHFR_braInt Brachyspira intermedia (bacteria) CP002874 Spirochaetes | >DHFR_braInt Brachyspira intermedia (bacteria) CP002874 Spirochaetes | ||
MIVSLIAAVDSKNGIGLNGVMPWGHIKEDMQFFRSTTTGYAVVMGRVTFESLGSKPLPNRKNIVISSSINNDLLEKYDNLFYESSFENTISKLLLEKNNQIFIIGGESIYKKALDYADIIYLTHIDKDYNCDRFFPQIDTKLFQSSKLKTFFHNDININIIKYTRTLL | MIVSLIAAVDSKNGIGLNGVMPWGHIKEDMQFFRSTTTGYAVVMGRVTFESLGSKPLPNRKNIVISSSINNDLLEKYDNLFYESSFENTISKLLLEKNNQIFIIGGESIYKKALDYADIIYLTHIDKDYNCDRFFPQIDTKLFQSSKLKTFFHNDININIIKYTRTLL | ||
=== Archaeal reference sequences === | |||
A small sampler of archaeal DHFR sequences is provided below, half of them best-blast to human and half to E coli. These lie entirely within euryarchaeotes; crenarchaeotes do not appear to have a relevant homolog and may use dihydropteridine reductase to conduct the reaction [http://www.ncbi.nlm.nih.gov/pubmed?term=14702303 as does] the bacterium Thermus thermophilus (which lacks DHFR in its completely sequenced genome). The extreme halophile, euryarchaeon Haloferax volcanii, has two DHFR, one of which is [http://www.ncbi.nlm.nih.gov/pubmed?term=10760149 adjacent to but not fused with] thymidylate synthase. The best percent identity 29.3% (human DHFR to Haladaptatus paucihalophilus) is significantly higher than any bacterial match to human, consistent with eukaryotes having a closer phylogenetic association with archaea than to bacteria. | |||
10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 | |||
| | | | | | | | | | | | | | | | | | | |||
DHFR_homSap MVGSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIP-EKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND | |||
DHFR_halPau -MTRLVCIAAVAENGVIGRDGDMPWH-YSADLRHFKETTMGHPV-------VMGRTTYESIAGQLDGPLPGRTNVVLSRSNPDLPEEV-VLVHSVEEAIETVEELD-----DTAFVIGGATVYEQFLPHAD--ELILTEIHDEYEGDTHFPEWDPKEW----------TEVERDEREELDFVRYER-- | |||
Consensus mgrLnCIaAVa#NggIGr#GD$PWh lra#lRhFqrmTmghpV !MGrkTweSIa #l#rPLkGRiNlVLSRenk#lP#ea fLsrSl##AieltE#l# Dmaf!!GGasVYe#a$nHad eLilTrIh##%EgDThFPEiDleew s#V#r#ereeldFerYEr | |||
>DHFR_halPau Haladaptatus paucihalophilus (archaea) ZP_08042706 euryarchaeote | |||
MTRLVCIAAVAENGVIGRDGDMPWHYSADLRHFKETTMGHPVVMGRTTYESIAGQLDGPLPGRTNVVLSRSNPDLPEEVVLVHSVEEAIETVEELDDTAFVIGGATVYEQFLPHADELILTEIHDEYEGDTHFPEWDPKEWTEVERDEREELDFVRYER | |||
>DHFR_halVol Haloferax volcanii (archaea) YP_003535331 euryarchaeote | |||
MELVSVAALAENRVIGRDGELPWPSIPADKKQYRSRIADDPVVLGRTTFESMRDDLPGSAQIVMSRSERSFSVDTAHRAASVEEAVDIAASLDAETAYVIGGAAIYALFQPHLDRMVLSRVPGEYEGDTYYPEWDAAEWELDAETDHEGFTLQEWVRSASSR | |||
>DHFR_natPel Natrinema pellirubrum (archaea) ZP_08963336 euryarchaeote | |||
MSEDRDVTEAAAALEPARELVGIVAVAENGVIGRDGDMPWHVPADLQHFKETTMGHPVIMGRVTYEGILETLGEPLPGRTTVVLTSRDLETPEGAVVAHDLESALERAETAARERHDDADRIFVAGGATVYEQFLPALDRLIVTEIHDDPEGDTSFPEWDRSEWDVVSRDDRDGFAFLEYVRRD | |||
>DHFR_halMed Haloferax mediterranei (archaea) YP_006350571 euryarchaeote | |||
MSEHTDDSVRFVLVAAVAENRVIGRDGDMPWHLPEDLKHFKATTMGHPVVMGRTTYESIARQIDGPLPGRRNIVLTSRDLDLPAGAETVDSVDEAVTAAEAAADDMGVETVYVVGGATVYEQFLDRASGLILTELDEAYEGDTYFPEWRDTGDETSWFEVDRDDRDGFSFVEYERRGDVAESEGAR | |||
>DHFR_natMag Natrialba magadii (archaea) YP_003481102 euryarchaeote | |||
MTVNTGADTDPVVEPDHELVGIVAVADNGVIGKDGDMPWHIPEDLQHFKETTMAHPVIMGRVTYESIVDALGEPLPGRTTVVLTSRDLETPENAVVAHDLQSAVEEAAAAADERHDGADRVFVAGGATVYEQYLPALDRLIVTEVHEEPDGDTQFPDWDRTDFDEVERDEHDGFAFVEYVRTP | |||
>DHFR_natPha Natronomonas pharaonis YP_327110 | |||
MKLVLIAAVAENGVIGTDGEMPWHYPEDLKRFKETTMGHPVIMGRTTYESITGQLGGPLPGRTNIVLSSRESLPIPEEVVHARDIEAALSAAEDALDADKQTVYVAGGATVYEQCLDRADELRITEVPESPDGDTYFPAIGDEWTATEREAGDEVAFVRYERAGD | |||
>DHFR_halLac Halobiforma lacisalsi ZP_09949777 | |||
MAGEPNSETISALEAAEATDRELVGIVAVADNGVIGADGEMPWHLPADLAHFKETTMDHPVIMGRVTYEGILETLGDPLPGRTTVVLTSQDLETPENAVVANGLTEAVERADRAARERHDGADRIFVAGGATVYEGFLPVLDRLVVTEIHDDPEGDAYFPAIDPEDWIEADRDERDGFAFVEYVRRD | |||
>DHFR_halWal Haloquadratum walsbyi YP_005840239 | |||
MNNINSDDRSPTDGSERTHNQTTDHDVDIDIDIDIDIVLIAAVAANDIIGRDGEMPWHIPADLQQFKRRTMGHPVILGRRTYEAIIGALGEPFPGRTSIVLSSQSRDVPADVILVHSITSAIREASKNARERGVGTVYVAGGGGVYEQFLPLADRLRLTELHDEYEGDTKFPSWDDTVWQETTREKNETFDFVTYERTP | |||
>DHFR_halBor Halogeometricum borinquense YP_004036472 | |||
MNESDSIDRDTTDANAESIEYVLVAAVAENGVIGRDGRMPWHFSEDMAHFKQTTMGHPVILGRKTYENIVDAIGEPFPGRMSIVLSSRELDLPEGAVLANSIKEATERAESVAEEMGVETVYVVGGARVYAQFLPRATRMILTEIHDSYDGETEFPEWEEAMWIETDRDERESFDFVTYERVE | |||
>DHFR_halXan Halopiger xanaduensis YP_004597600 | |||
MSEDDESTPGAAESALAAETDRELVGIVAVADNGVIGKDGDMPWHIPADLQHFKETTMDCPVIMGRVTYEGILEALGEPLPGRTTVVLTSRDLETPENAVVANDLAEAVEAAETAARERHDGADRAFVAGGATVYEQFLPALDRLIVTEVHDAPDGDTVFPDWDRDAWTETDRDEHDGFAFVEYVRERA | |||
=== Duplicated DHFRL1 in primates === | === Duplicated DHFRL1 in primates === |
Latest revision as of 14:17, 10 October 2012
Alignment of vertebrate DHFR
10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 | | | | | | | | | | | | | | | | | | | DHFR_homSap MVGSLNCIVAVSQNMGIGKNGDLPWPPLR--NEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND--- DHFR_panTro MVGSLNCIVAVSQNMGIGKNGDLPWPPLR--NEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELASKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND--- DHFR_gorGor MVGSLNCIVAVSQNMGIGKNGVLPWPPLR--NEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND--- DHFR_ponAbe MVGSLNCIVAVSQNMGIGKNGDLPWPPLR--NEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND--- DHFR_nomLeu MVGSLNCIVAVSQNMGIGKNGDLPWPPLR--NEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNYPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEFPGVLSDVQEEKGIKYKFEVYEKND--- DHFR_macMul MVSSLNCIVAVSQNMGIGKNGDLPWPPLR--NEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLARSLDDALKLTEQPELANKVDMIWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFLPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND--- DHFR_papAnu MARSLNCIVAVSQNMGIGKNGDLPWPPLR--SEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLARSLDDALKLTEQPELANKVDMIWIVGGNSVYKEAMNHPGHLKLFVTRIMQDFESDTFLPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND--- DHFR_calJac MVQLLNCIVAVSQNMGIGKNGELPWPPLR--NEFRYFQRMTTTSSVEDKQNLVIMGRKTWFSIPEKNRPLKDRINVVLSRELKEPPQGA-LLARSLDDALKLTEQPELANKVDMIWIIGGSSVYKEAMDHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPQYPGVPSDDQEEKGIKYKFEVYEKND--- DHFR_saiBol MVQLLNCIVAVSQNMGIGKNGDLPWPPLR--NEFRYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINVVLSKELKEPPQGAHFLARSLDDALKLTEQPELANKVDMIWIIGGSSVYKEAMDHPGHLKLFVTRIMQDFESDTFFPEIDLKKYKLLSEYPGVLSDVQEEKGIKYKFEVYEKND--- DHFR_tarSyr MVRPLYCIVAVSQNMGIGKDGTLPWPPLR--NEFRYFQRMTTMSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKVPPPGAHFLAKSLDDALKLIEQPELANKVDKVWIVGGNSVYREAMSQPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLSEYPGVLSDVQEEKGIKYKFEVYEKND--- DHFR_otogar MVRPLNCIVAVSQNMGIGKNGDLPWPPLR--NEFNYFQRMTKTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKESPQGAHFLAKSLDDALKLIEEPELANKVDMVWIVGGSSVYKEAMNQPGHVKLFVTRIMQDFECDTFFPEIDLKKYKLLSEYPGVLSDVQEEKGIKYKFEVYEKND--- DHF1_micMur MVRTLNCIVAVSQNMGIGKNGDLPWPPLR--NEFRYFQKMTTTSSVEGKQNLVIMGRKTWFSIPEKSRPLKDRINVVLSRELKEPPQGAHFVAKSLDDALKLIEQPELANKVDMVWIVGGSAVYKEAMNQPGHLKLFVTRIMQDFESDTFFPEIDLKKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND--- DHFR_tupBel MVRPLNCIVAVSQNMGIGKNGDLPWPPLR--NEFKYFQRMTTTSSVEGKQNIVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLAKSLDDALKLIEQPELANQVDMVWIVGGSSVYREAMNQPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND--- DHFR_musMus MVRPLNCIVAVSQDMGIGKNGDLPWPPLR--NEWKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQPELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEYPGVLSEVQEEKGIKYKFEVYEKKD--- DHFR_ratNor MVRPLNCIVAVSQNMGIGKNGDLPWPLLR--NEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPQGAHFLAKSLDDALKLIEQPELASKVDMVWVVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLEKYKLLPEYPGVLSEIQEEKGIKYKFEVYEKKD--- DHFR_criGri MVRPLNCIVAVSQNMGIGKNGDFPWPMLR--NEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPQGAHFLAKSLDDALKLIEQPELADKVDMVWIVGGSSVYKEAMNQPGHLRLFVTRIMQEFETDTFFPEIDLEKYKLLPEYPRVLPEVQEEKGIKYKFEVYEKKG--- DHFR_perMan MVRPLNCIVAVSQNMGIGKNGDLPWPPLR--NEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPQGAHFLAKSLDDALKLIEQPELANKVDMVWIVGGSSVYKEAMNKPGHLRLFVTRIMQEFESDTFFPDIDLEKYKLLPEYPGVLSEVQEEEGIKYKFEVYEKKA--- DHFR_perPol MVRPLNCIVAVSQNMGIGKNGDLPWPLLR--NEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPQGAHFLAKSLDDALKLIEQPELANKVDMVWVVGGSSVYKEAMNKPGHLRLFVTRIMQEFESDTFFPDIDLEKYKLLPEYPGVLSEVQEEEGIKYKFEVYEKKA--- DHFR_dipOrd MGRSLNCIVAVSQNMGIGKNGDLPWPPLR--NEFKYFQRMTTTSTVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPQGAHFLAKSLDEALKLIEQPELNNKVDMVWIVGGSSVYKEAMNQPGHHKLFVTRIMQEFESDTFFPEIDLEKYKLLPEYPGVLSEVQEEKGIKYKFEVYEKID--- DHFR_speTri MVRPLNCIVAVSQNMGIGKNGDLPWPPLR--NEFKYFQRMMTTPSVEGKQNLVIIGRKTWFSIPEKNRPLNDRINIVLSRELKELPKGAHFLAKSLTDALKLTEQPELASKIDMIWIVGGSSVYKEAMIQPGHIKLFVTRIMQEFESDTFFPEIDLEKYKLLPEYPGILSEVQEEKGIKYKFEVYEKTD--- DHFR_cavPor MVRPLNCIVAVSQNMGIGKNGDLPWPPLR--NEFGYFQRMTTTPSVEGKQNLVIMGRKTWFSLPEKSRPLKDRINVVLSRELKEPPQGAHFLAPSLDDALKLIEQPGLANEVDMVWIVGGSSVYKEAMERPGHIKLFVTRIMQEFESDTFFPEIDLKKYRLLPEYPGVPSEIQEEKGIKYKFEVYEKND--- DHFR_oryCun MVRPLNCIVAVSQNMGIGKNGDLPWPPLR--NEFRYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINVVLSRELKEPPQGAHFLAKSLADALKLIEEPELAEKVDMAWIVGGSSVHKEAMNQPGHLRLFVTRIIQEFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND--- DHFR_ochPri MVRPLNCIVAVSQNMGIGRNGDLPWPPLR--NEFKYFQRMTTTSTVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPPGAHFLAKSLDDALKLIEEPELAERVDMVWIVGGSSVYKEAMDKPGHLRLFVTRIMQEFESDTFFPEIDLEKYKLLPEYPGILPEVQEERGIKYRFEVYEKND--- DHFR_felCat MVRPLNCIVAVSQNMGIGKNGDLPWPPLR--NEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRDLKEPPQGAHFLAKSLDDALELTEQPELAHKVDMVWVVGGSSVYKEAMNRPGHVRLFVTRIMQEFESDTFFPEIDLEKYKLLPEYPGVLSDIQEEKSIKYKFEVYEKNN--- DHFR_canFam MVRTLNCIVAVSQNMGIGRNGDLPWPPLR--NEFKYFQRMTTNSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRDLKEPPQGAHFLAKSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNKPGHLRLFVTRIMHEFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND--- DHFR_vulVul MVRTLNCIVAVSQNMGIGRNGTVPWPPLR--NEFKYFQRMTTNSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRDLKEPPQGAHFLAKSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNKPGHLRLFVTRIMHEFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND--- DHFR_musPut MVRTLNCIAAVSQNMGIGKNGDLPWPPLR--NEFKFFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRDLKEPPQGAHFLAKSLDDALKLIEQPELANKVDMVWIVGGSSVYKEAMNKPGHLRLFVTRIMQEFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND--- DHFR_ailMel MVRTLNCIAAVSQNMGIGKNGDLPWPPLR--NEFKFFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRDLKEPPQGAHFLAKSLDDALKLIEQPELANKVDIVWIVGGSSVYKEAMNKPGHLRLFVTRIMQEFESDTFFPEIDLDKYKLLPEYPGVPSDVQEEKGIKYKFEVYEKND--- DHFR_equCab MVRPLNCIVAVSQNMGIGKNGDLPWPPLR--NEFKYFQRMTTASSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLAKSLDDALSLLEQPELANKVDMVWIVGGSSVYKEAMNKPGHLRLFVTRIMQEFESDTFFPEIDFEKYKLLPEYPGVLSDIQEEKGIKYKFEVYEKND--- DHFR_susScr MVRPLNCIVAVSQNMGIGKNGDLPWPPLR--NEYKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPQGAHFLAKSLDDALKLTEQPELKNKVDMVWIVGGSSVYKEAMNKPGHIRLFVTRIMKEFESDTFFPEIDLEKYKLLSECSGVPSDVQEEKGIKYKFEVYEKNN--- DHFR_turTru MVRPLNCIVAVSQNMGIGKNGDLPWPPLR--NEYRYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPQGAHFLAKSLDGALKLIEQPELTNTVDMVWIVGGGSVYKEAMNKPGHLRLFVTRIMQEFESDTFFPEIDLEKYKLIPEYPGVPSDVQEEKGIKYKFEVYEKNN--- DHFR_oviAri MVRPLNCIVAVSQNMGIGKNGNLPWPPLR--NEFKYFQRMTTVSSEEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPKGAHFLAKSLDDALELIEDPELTNKVDMVWIVGGSSVYKEAMNKPGHLRLFVTRIMQEFESDVFFPEIDFEKYKLLPEYPGVPLDVQEEKGIKYKFEVYEKNN--- DHFR_capHir MVRPLNCIVAVSQNMGIGKNGNLPWPPLR--NEFKYFQRMTTVSSEEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPKGAHFLAKSLDDALELIEDPELTNKVDMVWIVGGSSVYKEAMNKPGHLRLFVTRIMQEFESDVFFPEIDFEKYKLLPEYPGVPSDVQEEKGIKYKFEVYEKNN--- DHFR_bosTau MVRPLNCIVAVSQNMGIGKNGNLPWPPLR--NEFQYFQRMTTVSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPKGAHFLAKSLDDALELIEDPELTNKVDVVWIVGGSSVYKEAMNKPGHVRLFVTRIMQEFESDAFFPEIDFEKYKLLPEYPGVPLDVQEEKGIKYKFEVYEKNN--- DHFR_myoLuc MVRTLNCIAAVSQNMGIGKNGDLPWPPLR--NEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPKKNRPLKDRINLVLSRELKEPPKGAHFLAKSLDDALKLIEEPELTNQVDMVWIVGGSSVYKEAMSKPGHQRLFVTRIMQEFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKNE--- DHFR_pteVam MVLSLNCIVAVSQNMGIGKNGDLPWPPLR--NEFKYFQRMTTTSSVEGKRNLVIMGRKTWFSIPKKNRPLKDRINLVLSRELKEPPKGAHFLAKSLDDALKLIEQPELRSKVDMVWIVGGSSVYKEAMNKPGHIKLFVTKIMQEFESDIFFPEIDLGKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND--- DHFR_sorAra MVRPLNCIVAVSQNMGIGKNGELPWPPLR--NEFTYFRKMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPTGAHFLAKSLDDALKLIEQPELTNKVDMVWIVGGSSVYQEVMNKPGHLRLFVTRIMQEFECDTFFPEIDLEKYKLLTDYPGVVSDVQEEKGIKYKFEVYEKKD--- DHFR_loxAfr MVRPLNCIVAVSQNMGIGKNGDLP-PPLR--NEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRGLKEPPQGAHFLAKSLDDALKLIEQPELRNKVDMVWIVGGSSVYKEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLEKYKRLSEYPGVLSDVQEEKGIKYKFEVYEKKD--- DHFR_proCap MVRPLSCIVAVSQNMGIGKNGDLPWPPLR--NEFKHFRTMTSTPSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLAKSLDDALKLIEQPELTNKVDLVWIIGGSSVYKEAMSQPGHLTLFVTRIMQEFESDTFFPEIDLEKFKLLPEYPGVPSDVQEEKGIKYKFEVYEKKD--- DHFR_monDom MVRQLNCIAAVSKNMGIGKDGDLPWPLLR--NEFKYFQKMTTTPSVEGKQNLVIMGKKTWFSIPEKCRPLKDRINVVLSRELKEPPQGAHYLASSLDNALNLLELPELADKVDMVWIVGGSSVYKEAMNKPGHQRLFITRILQEFESDTFFPEIDLKKYKQLQDYPGVSPDVQEEKGIQYKFEVYEKHE--- DHFR_macEug MAPTINCIVAVAQNMGMGKNGDLPWPPLR--KEFQYFQKMTTTPSVEGKQNLLIMGKKTWFSIPEKNRPLKDRINLVLSRELKDPPLGAHYLAKSLDDALDLLRLPELANTVDMVWVVGGSSVYKEAMNKPGHQRLFVTRILQEFESDTFFPEIDLKRYKLLPEYPGVLTDVQEEKGIQHKCEIHEKHD--- DHFR_sarHar MVRPINCIAAVSQNMGIGKNGDLPWPPLR--NEFNYFQKMTTTSSVEGKQNLLIMGKKTWFSIPEKHRPLKDRINLVLSRELKEPPPGAHYLAKSLDDALDLLELPELADKVDMIWVIGGSSVYKEAMEKPGHQRLFVTRILQEFECDTFFPEIDLKKYKLLPEYPGVLTDIQEEKGIQYKFEVYEKHD--- DHFR_triVul MVRTINCIVAVSQNMGIGKNGDLPWPPLR--NDFKHFQKMTTIPSVEGKQNLLITGKKTWFSIPEKSRPLKDRINVVLSRELKEPPPGAHYLAKSLDDALDLLELPELANKVDMIWVVGGSSVYKEAMNKRGHNRLFVTRILQEFESDTFFPEIDLKRYKLLXEYPGVLTDVQEEKGIQYKFEVYEKHD--- DHFR_ornAna MGPLLNCIVAVAKNGGIGNKGDLPWPALR--NEFRYFQKMTTTPTVKGKQNLVIMGKKTWFSIPEKSRPLKDRINIVLSRELKEPPQGAHYVAKSLDDALNLLHLPELASKVDMVWIIGGSSVYKEAMEKGGHQRLFVTRILQDFESDTFFPEIDLEKYKLIPEYPGVPADTQEEKGIQYRFEVYEKHI--- DHFR_tacAcu MGRPLNCIAAVAKNGGIGNKGDLPWPPLR--NEYRYFQKMTTTPTVEGKENLVIMGKKTWFSIPEKSRPLKDRINIVLSRELKEPPQGAHYVAKSLDDALNLLNLPELASKVDMVWIIGGSSVYKEAMEKPGHQRLFVTRILQEFESDTFFPEIDLEKYKLIPEYPGVPADTQEEKGIQYRFEVYEKHI--- DHFR_galGal MVRSLNSIVAVCQNMGIGKDGNLPWPPLR--NEYKYFQRMTSTSHVEGKQNAVIMGKKTWFSIPEKNRPLKDRINIVLSRELKEAPKGAHYLSKSLDDALALLDSPELKSKVDMVWIVGGTAVYKAAMEKPINHRLFVTRILHEFESDTFFPEIDYKDFKLLTEYPGVPADIQEENGIQYKFEVYQKSVLAQ DHFR_lagLag MVRSLNSIVAVCQNMGIGKDGNLPWPPLR--NEYKYFQRMTSTSHVEGKQNALIMGKKTWFSIPEKNRPLKDRINIVLSRELKEAPEGAHYLSKSLDDALALLDSPELKSKVDMVWIVGGTSVYKAAMEKPINHRLFVTRILHEFESDTFFPEIDYKDFKLLTEYPGVPADVQEENGIQYKFEVYQKSVLAQ DHFR_anaPla MVRSLNSIVAVCQNMGIGKDGNLPWPPLR--NEYKYFQRMTSTSHVEGKQNALIMGKKTWFSIPEKNRPLKDRINIVLSRELKEAPEGAHYLSKSLDDALALLDSPELKSKVDMVWIVGGTSVYKAAMEKPINHRLFVTRILHEFESDTFFPEIDYKDFKLLTEYPGVPADVQEENGIQYKFEVYEKTVLAQ DHFR_taegut MPRSLNSIVAISQNMGIGKDGRLPWPPLR--NDYKYFQRMTSASPVEGKKNAVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEAPKGAHYLSKSLDDALALLDSPELQSKVCMVWIVGGSSVYKEAMEKPIHHRLFVTRILKEFESDTFFPEIDHKDYKLLTEYPGVPADIQEENGIQYKFEVYEKTVVP- DHFR_ficHyp MVRSLNSIVAVSQNMGIGKDGRLPWPPLR--NEYKYFQRMTSTPRVEGKQNALIMGKKTWFSIPEKNRPLKDRINIVLSRELKEAPEGAHYLSKSLDDALALLDSPELKSKVDMVWIVGGTSVYKAAMEKPIHHRLFVTRILKEFESDTFFPEIDHKDYKLLTEYPGVPADIQEENGIQYKFEVYEKAVV-- DHFR_melUnd MVRSLNSIVAVCQNMGIGKDGSLPWPPFR--NEYKYFQRMTSTSQVEGKQNALIMGKKTWFSIPEKNRPLKDRINIVLSRELQETPKGAHYLSKNLDDALALLDSPELKSKVDMVWIVGGTSVYKAAMEKPINHRLFVTRILQEFESDTFFPEIDHKDYKLLTEYPGVPADIQEENGIQYKFEVYEKAV--- DHFR_allMis MVSSLNAIAAVSQNMGIGKNGTLPWPPLR--NEFKYFQRMTTTPSVPGKQNVLIMGKKTWFSIPEKNRPLKDRINIVLSRELAEVPKGAHHLARSLDDALVLLDSPELKNKIDLVWIVGGSSVYKAAMEKPINQRLFVTRILHEFESDTFFPEIDLEKYRLLPEFPGVPADVQEENGIHYKFEVYEKTV--- DHFR_croPor MVSSLNAIAAVSQNMGIGKNGTLPWPPLR--NEFKYFQRMTTTPSVPGKQNVLIMGKKTWFSIPEKNRPLKDRINIVLSRELAEVPKGAHHLARSLDDALVLLDSPELKNKIDLVWIVGGSSVYKAAMEKPINQRLFVTRILHEFESDTFFPEIDLEKYRLLPEFPGVPADVQEENGIHYKFEVYEKTV--- DHFR_chrPic MVRPLNCIAAVCQNMGIGKNGDLPWPPLR--NEFKYFQRMTTTTTVEGKQNVLIMGKRTWFSIPEKNRPLKDRINIVLSRELKETPKGAHYLAKSLDDALVLLDSPELKSKIDMVWIIGGSSIYKAAMEKPVHQRLFVTRILHEFESDTFFPEIDLEKYKHLPEYPGVPADVQEENGIRYKYEVYEKTV--- DHFR_anoCar MVLSLNSIAAVCQNMGIGKNGQLPWPPLR--NEFKYFQKMTMTPTQEGKQNVVIMGKRTWFSIPEKNRPLKDRINIILSREMKEVPKGAHYLARSLDEALDLLQSPELENKIDLVWIVGGSSVYKAAMDKPIHHRLFVTRILHDFESDTFFPEIDLEKYKLIPSYAGVPTDIQEENGIRYRFEVYEKNI--- DHFR_pytMol MVASLHSIVAVCNNMGIGKDGKLPWPPLR--NEFKHFQKMTMTTKEEGKQNVVIMGKKTWFSIPEKHRPLKNRINVVLSKELKDVPEGAHYLAKSLEEALDLLETSEMERKVDKVWIVGGSSVYKAAMEKPIHHQLFVTRIMHDFESDTFFPEIDLKKYKLLPKYTGIPTDIQEENGIQYKFEVYENTI--- DHFR_ambMex MGRILNAIVAVCPSMGIGKDGNLPWPILR--NEFKYFQRMTMTATQEGKQNVVIMGRKTWLSIPEKNRPLKGRINIVLSRELTAPPPGAHYLANSLDGALELLETRELAGKVDLLWIIGGSSLYRELIGRPHHQRLFVTRILKEFECDTFLPEFDLEKYRLLPEYPGVSLGIQEENGIQYKFEVYEKVEEA- DHFR_xenTro MNPFLHAVVAVCPNQGIGKEGSLPWPLLR--NEFKHFQRLTMTPTVEDKKNVVIMGRKTWFSIPEKNRPLKERINIVLSKELKEPPTGAHYLSKSLAEAIDLLEDPELKDKVDLVWVIGGSSLYQELMEKPVNQRWFVTRILQEFECDTYLPEIDLNSFRLLPEYPGISPELQEENGVQYKFEVYEKIV--- DHFR_xenLae MNQFLHAVVAVCPNQGIGKGGSLPWPLLR--NEFKHFQRLTMTPTVEGKKNVVIMGRKTWFSIPEKNRPLKERINIVLSKELKEPPTGAHYLSKSLTEAIDLLEDPELKDKVDLVWIIGGSSLYQELMEKPINQRWFVTRILQEFECDTYLPEINLNSFQLLPQYPGISPELQEENGVQYKFEVYEKIV--- DHFR_ranCat MNQFLHAVVAVCPNQGIGKGGTLPWPLLR--NEFKHFQRLTMTPTTEGKQNVVIMGRRTWFSIPEKNRPLKGRINIVLSRELTEPPNGAHYLAKSLDEAVHLLKTPEMKEKIDLIWIIGGSSLYKEVMDKPVSQRLFITRIQQEFECDTFLPDVDLQSYKLLPEYPGISSEMQEENGIQYKFEVYEKML--- DHFR_latCha MARLLNSIVAVCPNLGIGKDGNLPWHPKRLSNEFRYFQKMTTTPTVEGKQNVVIMGRKTWFSIPEKNRPLKGRVNVVLSRELQEAPEGAHYLARDLDSALKLLESPELADRVDLVWIIGGSALYKEALEHPSQHRLFVTRVLQQFECDTYLPEINFNKFTLLPEFPGVSSAIQEESGIQYKFEVYEKNS--- DHFR_gadMor MSRVLNCIVAVCPDAGIGYKGDLPWHPTRLNNEFKHFRRLTVTPGAEDKQNVVIMGRKTWNSIPEKNRPLNDRINIVLSRQLEAPPSGAHHLARDLPSALRLLDVPE-GLLADQVWLIGGSSMYSEVMETPCPRRLFVTRILKQFPSDTFFPDISPAQFRLLPEYPGVSSELQEENGIQYRFQVYQSVDP-- DHFR_tetNig MARVLNAIVAVCPDLGIGRNGDLPWHPIRLDNEFKHFRKMTSTPSVNGKQNVVIMGRKTWFSIPEKHRPLANRINIVLSRRSREPPAGAHHLAHDLSSALQLVET-QLAEQADQVWVIGGSSLYQEMMERPGTSRLFVTHVLKQFDCDTFLPEIRPQRYRLLPQFPGVPQELQEEKGIQYRYQVYESLEP-- DHFR_hipHip MSRILNGIVAVCPDLGIGNRGNLPWHPVRLSNEFKHFRSMTATPSEKGKQNVVIMGRKTWFSIPEKNRPLNNRINIVLSRQCKAPPAGAHHLSADFSSALRLVDT-ELADQADQVWVIGGSSLYKEMMESPGTRRLFVTRILKQFDCDTFLPEISPDKYRQLPEFPGVPQELREENGIQYRFEVYESIEP-- DHFR_solSen MSRVLNGIVAVCPDMGIGMTGNLPWHPVRLSNEFKHFRTMTATSSVKGKKNVVIMGRKTWFSIPEKNRPLNNRINIVLSRQCKLPPAGAHHLAADFSSALRLVET-ELADEADQIWIIGGSSLYKEMMESPGIKRLFVTRILKQFECDTFLPEISLDKYRLLPEFAGVSQELQEENAIQYRFEVYESVEQ-- DHFR_oreNil MPRVLNAIVAVCPDRGIGNKGNLPWHPIRLSKEFAHFRKMTATPSVKGKQNVVIMGKKTWYSIPEKNRPLSNRINIVLSRECKVPPAGAHYLASDFSSALRLIDT-ELADQADQVWVIGGSSLYKEMMGSTGMRRLFVTQILKQFECDTFLPEISLDKYRLLPEFPDVPQELQEENGIQYRFKVYESIQE-- DHFR_dicLab MSRILNGIVAVCPDLGIGMNGNLPWHPVRLGNEFKHFRTMTATPSVKGKQNVVIMGRKTWFSIPEKNRPLNNRINIVLSKECKVPPAGAHHLAADFNSALRLVET-ELADQADQVWVIGGSSLYKELMESPGTRRLFVTRILKQFECDTFLPEICPDKYQLLPEFPGVSQELQEENGIQYRFEVYESTEQ-- DHFR_perFla MSRILNGIVAVCPDLGIGNNGNLPWHPVRLNNEFKHFRTMTATPSVNGKQNVVIMGRKTWFSIPEKNRPLNNRINIVLSRECKAPPAGAHHLARDFSSALRLVDT-ELAQQADTVWVIGGSSLYKEMMESPGTRRLFVTRILKQFECDTFLPEITPDKYRLLPEFPGVPQELQEENGIQYRFEVYESIQQ-- DHFR_spaAur MSRIVNGIVAVCPDLGIGNNGNLPWHPVRLNREFKHFRTMTATPSVKGKQNVVIMGRKTWFSIPDKFRPLNNRINIVLSRECKVPPAGAHHLAADFSSALSLVDT-ELADQADQVWVIGGSSLYKELMESTRTSRLFVTRILKQFECDTFLPEISPDKYSLLPEFPGVPQGLQEENGIQYKFEVYERIKQ-- DHFR_gasAcu MSRVLNGIVAVCPDLGIGCHGNLPWHPLRLSNEFKHFRRMTATASVKDKQNVVIMGRKTWFSIPEKNRPLNNRINIVLSRELKAPPAGAHHLAPDFSSALRLVDT-ELAEQADQVWVIGGSSLYKELLESPGTRRLFVTRILKQFECDTFLPEICPDRYRLLPEFPGVPEELQEENGVQYRFEVYESIDV-- DHFR_oryLat MTRTLNGIVAVCPDLGIGKGGNLPWHPLRLSKDFALFRKMTSTPLVAGRQNVVIMGRKTWFSIPEKNRPLQNRINIVLSRQLKAPPAGAHFVAADFAAALRLVDS-ELAERADQVWIIGGSSIYXEMMESPAPRRLIVTRVQKQFECDTFFPEIRPEQFRLLPEFPGVSQELQEENGVSYKFEVYESIQQ-- DHFR_anoFim MSRVLNAIVAVCPDLGIGRNGDLPWHPVRLNNEFKHFRRMTSTPSVEGKQNVVIMGRKTWFSIPEKNRPLNNRINIVLSRELKTPPAGAHHLAQDFNSALRLVDM-KLAEQVDQVWVIGGSSLYKELMESQGIRRLFVTQILKQFECDTFLPKISPDKYRLLPEFPGVPQELQEENGIQYRFEVYESIEQ-- DHFR_esoLuc MSRVLNCIVAVCPNMGIGNKGNLPWHPKRLNNEFKYFQKMTMTPFVEGKQNVDIMGRKTWFSIPERNRPLKNRINIVLSRELKEPPPGAHHLASDFSSALRLLDTAEMAERVDQVWVIGGSSLYKEMMESEGIRRLFVTRILQQFESDTFLPEINQDKYRLLPEFPGVPQDVQEESGIQYRFEVYESVDH-- DHFR_salSal MSRVPNCIVAVCPDVGIGNNGNLPWHPKRLNNEFKYFQKMTMTSSVEGKQNVVIMGRKTWFSIPERNRPLKNRINIVLSRELKKPPAGAHHLASDFRSALCLLDMAEMAERADQVWVIGGSSLYKEMMESQGTRRLFVTRILQQFKSDTFLPEINQDQYRLLPEFPGVPQDLQEEAGLQYRFEVYESIDQ-- DHFR_oncMyk MSRVLNCIVAVCPDMGIGNNGNLPWHPKRLNNEFKYFQKMTMTSSVEGKQNAVIMGRKTWFSIPERNRPLKNRINIVLSRELKEPPAGAHQLASDFRSALRLLDTAEMAERVDQVWVIGGSSLYEEMMESQGTRRLFVTRILQQFKSDTFLPEINQDKYRLLPEFPGVPQDLQEEAGLQYRFEVYESIDQ-- DHFR_danRer MSRILNCIVAVCPDMGIGKNGNLPWHPIRLSNELKHFQKMTMTPSDEGKKNVVIMGRKTWFSIPAAHRPLKNRINIVLSRELKTAPEGAHYLASDFSSALHLLDSGELEKLVDQVWIIGGSSLYKEVMERSGHRRLFVTRILKQFDCDTFIPNFDMDKYKLLPEFPGVPVGLQEDNGVQYLFEVYESIKH-- DHFR_cteIde MSRILNCIVAVCPDMGIGRKGNLPWHPIRLSNEFKHFQKMTMTPSVEGKKNVVIMGRKTWFSIPAQNRPLKNRINIVLSRELKTAPEGAHYLASDLSSALHLLDSSELEKQVDQVWIIGGSALYKEVMERSGPRRLFVTRVLKQFDCDTFIPEINMDKYKLLPEFPGVPTGLQDENGVQYVFECMIVS---- DHFR_cypCar MSRILNSIVAVCPDMGIGKNGNLPWHPIRLSNEFKHFQKMTMTPLVEGKKNVVLMGRKTWFSIPAANRPLKNRINIVLSRELKTAPEGAHYLASDFSSALHLLDSSELEKQVDQVWIIGGSALYKEVMESSGPRRLFVTRVLKQFDCDTFIPDINMDKYKLLPEFPGVPTGLQEENGVQYVFEVYESTDH-- DHFR_ictPun MGRVLNCIVAVCPDMGIGRNGNLPWHPIRLSKEFKHFQKMTMTPTVEGKKNVVIMGRKTWFSIPAQNRPLKNRINVVLSRELKTAPEGAHYLAADFSSALRILENAELEAQADQVWIIGGSSLYKEVMESSAHQRLFVTRILKQFDCDTFIPNIDLDKFRLLPEFPGVPAGLQEENGLQYVFEVYESVEQ-- DHFR_leuEri MTRLINSIVAVCPNMGIGNNGNFPWHPIRLSKEFKHFQRMTSTPSVEGKCNAVIMGRKTWHSIPEKNRPLKNRINIVLSRELKEVPEGAHYLAPDLDSALALLDSSDLQDKVDLVWIIGGSALYKEALESPASHRLFVTRILQDFDSDTFIPEINLNKFKLLPEFPDVPADIQEENGIRFKYEVYERISPV- DHFR_squAca MPRLVNCIVAVCPNMGIGKDGNFPWHPIRLSKELKHFQKMTATPSVEGKRNAVIMGRKTWFSIPEKHRPLKDRLNIVLSRELKELPHGANYLAHDLDSALAHLDSSELQDKVDLVWIIGGSTLYKEAMESPVSHRIFVTRILKDFESDTFLPEMDLNKFKLLPKFPDVPTEVQEDNGIQYKFEVYERIAHA- DHFR_eptBur MQNPVNVIAAVLPNMGIGWKGGLPWHSKSLVKEMKHFTRLTSAAA-EGKQNAVVMGRKTWESIPEKFRPLRNRINVVLSRTLSHLPLGAHYLATDFSNALAQLSESELCCRIDHIWIIGGSSIYKEALLHPACHRIFITRIMHDFEADTFFPHIDSKRFSLCQSYPGIPEEIQEENGVQYKFEVYEVVAK-- DHFR_petMar -----------------------------------------------GKQNVVIMGRKTWFSIPEKKRPLKNRINIVLSRELKCLPEGAHYLVPDLNSALSLIDSPAIQEKLDGVWILGGGGVYKEAMEHSACRRLFITRVLQTMEADAFFPDIDADKFKLLP----------------------------- 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 | | | | | | | | | | | | | | | | | | | DHFR_homSap MVGSLNCIVAVSQNMGIGKNGDLPWPPLR--NEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND--- DHFR_panTro .............................................................................................................S.................................................................................. DHFR_gorGor .....................V.......................................................................................................................................................................... DHFR_ponAbe .......................................................................D........................................................................................................................ DHFR_nomLeu .................................................................................................................................Y..................................F........................... DHFR_macMul ..S.........................................................................................A.....................I...................................L......................................... DHFR_papAnu .AR............................S............................................................A.....................I.....N.............................L......................................... DHFR_calJac ..QL.................E.........................D........R..............D...V.............-L.A.....................I..I..........D..................................Q....P..D.................... DHFR_saiBol ..QL....................................................R..............D...V...K............A.....................I..I..........D...........................K.....S............................. DHFR_tarSyr ..RP.Y.............D.T....................M.............R..............D...........V..P.....AK........I..........K......N...R...SQ................................S............................. DHFR_otogar ..RP..............................N......K..............R..............D...I........S.......AK........I.E........................Q...V............C.........K.....S............................. DHF1_micMur ..RT..................................K.................R.........S....D...V...............VAK........I..................A.......Q..........................K................................... DHFR_tupBel ..RP..............................K................I....R..............D....................AK........I.......Q.............R....Q.............................................................. DHFR_musMus ..RP.........D...................WK.....................R..............D...I..........R.....AK......R.I......S..............Q....Q....R........E............G.............E................K.... DHFR_ratNor ..RP......................L.......K.....................R..............D...I................AK........I......S......V.......Q....Q....R........E..........................EI...............K.... DHFR_criGri ..RP..................F...M.......K.....................R..............D...I................AK........I......D...................Q....R........E..T...................R..PE................KG... DHFR_perMan ..RP..............................K.....................R..............D...I................AK........I..........................K....R........E........D.................E....E...........KA... DHFR_perPol ..RP......................L.......K.....................R..............D...I................AK........I.............V............K....R........E........D.................E....E...........KA... DHFR_dipOrd .GR...............................K.........T...........R..............D...I................AK...E....I.....N....................Q...H.........E..........................E................I.... DHFR_speTri ..RP..............................K.....M..P..........I.R.............ND...I........L.K.....AK..T............S.I..I.............IQ...I.........E.......................I..E................T.... DHFR_cavPor ..RP..............................G........P............R.....L...S....D...V................AP........I...G...E.................ER...I.........E............K..R........P.EI.................... DHFR_oryCun ..RP....................................................R..............D...V................AK..A.....I.E....E....A........H.....Q....R......I.E................................................ DHFR_ochPri ..RP..............R...............K.........T...........R..............D...I..........P.....AK........I.E....ER.................DK....R........E.......................I.PE....R....R........... DHFR_felCat ..RP..............................K.....................R..............D...I....D...........AK......E........H......V............R...VR........E...........................I....S...........N... DHFR_canFam ..RT..............R...............K.......N.............R..............D...I....D...........AK...................................K....R.......HE................................................ DHFR_vulVul ..RT..............R..TV...........K.......N.............R..............D...I....D...........AK...................................K....R.......HE................................................ DHFR_musPut ..RT....A.........................KF....................R..............D........D...........AK........I..........................K....R........E................................................ DHFR_ailMel ..RT....A.........................KF....................R..............D........D...........AK........I..........I...............K....R........E............D...........P....................... DHFR_equCab ..RP..............................K.......A.............R..............D....................AK......S.L..........................K....R........E...........F...............I.................... DHFR_susScr ..RP.............................YK.....................R..............D...I................AK..............K....................K...IR.......KE..................S.CS..P...................N... DHFR_turTru ..RP.............................Y......................R..............D...I................AK...G....I.....T.T.........G........K....R........E.................I......P...................N... DHFR_oviAri ..RP.................N............K.......V..E..........R..............D...I..........K.....AK......E.I.D...T....................K....R........E....V......F............PL..................N... DHFR_capHir ..RP.................N............K.......V..E..........R..............D...I..........K.....AK......E.I.D...T....................K....R........E....V......F............P...................N... DHFR_bosTau ..RP.................N............Q.......V.............R..............D...I..........K.....AK......E.I.D...T....V...............K...VR........E....A......F............PL..................N... DHFR_myoLuc ..RT....A.........................K.....................R.......K......D..............K.....AK........I.E...T.Q.................SK...QR........E............................................E... DHFR_pteVam ..L...............................K..............R......R.......K......D..............K.....AK........I.....RS...................K...I.....K...E....I.......G................................... DHFR_sorAra ..RP.................E............T..RK.................R..............D..............T.....AK........I.....T...............Q.V..K....R........E..C...............TD....V..................K.... DHFR_loxAfr ..RP....................-.........K.....................R..............D........G...........AK........I.....R....................Q....R........E................R.S........................K.... DHFR_proCap ..RP.S............................KH.RT..S.P............R..............D....................AK........I.....T....L...I..........SQ....T........E..............F.........P..................K.... DHFR_monDom ..RQ....A...K......D......L.......K...K....P......................C....D...V..............Y.AS...N..N.L.L....D...................K...QR..I...L.E............K...Q.QD....SP........Q........HE... DHFR_macEug .APTI......A....M..............K..Q...K....P........L..................D...........D..L...Y.AK......D.LRL.....T.....V............K...QR......L.E............KR...........T........QH.C.IH..H.... DHFR_sarHar ..RPI...A.........................N...K.............L.............H....D..............P...Y.AK......D.L.L....D....I.VI..........EK...QR......L.E..C.........K............T.I......Q........H.... DHFR_triVul ..RTI...........................D.KH..K...IP........L.T...........S....D...V..........P...Y.AK......D.L.L.........I.V............KR..NR......L.E............KR....X......T........Q........H.... DHFR_ornAna .GPL.......AK.G...NK......A...........K....PT.K...................S....D...I..............YVAK......N.LHL....S.......I..........EKG..QR......L...................I......PA.T......Q.R......HI... DHFR_tacAcu .GRP....A..AK.G...NK.............Y....K....PT....E................S....D...I..............YVAK......N.LNL....S.......I..........EK...QR......L.E.................I......PA.T......Q.R......HI... DHFR_galGal ..R...S....C.......D.N...........YK......S..H......A...................D...I........A.K...Y..K......A.LDS...KS..........TA...A..EK.INHR......LHE...........YKDF...T.....PA.I...N..Q......Q.SVLAQ DHFR_lagLag ..R...S....C.......D.N...........YK......S..H......AL..................D...I........A.E...Y..K......A.LDS...KS..........T....A..EK.INHR......LHE...........YKDF...T.....PA.....N..Q......Q.SVLAQ DHFR_anaPla ..R...S....C.......D.N...........YK......S..H......AL..................D...I........A.E...Y..K......A.LDS...KS..........T....A..EK.INHR......LHE...........YKDF...T.....PA.....N..Q........TVLAQ DHFR_taegut .PR...S...I........D.R..........DYK......SA.P....K.A....R..............D...I........A.K...Y..K......A.LDS...QS..C...............EK.I.HR......LKE...........HKD....T.....PA.I...N..Q........TVVP. DHFR_ficHyp ..R...S............D.R...........YK......S.PR......AL..................D...I........A.E...Y..K......A.LDS...KS..........T....A..EK.I.HR......LKE...........HKD....T.....PA.I...N..Q........AVV.. DHFR_melUnd ..R...S....C.......D.S.....F.....YK......S..Q......AL..................D...I......Q.T.K...Y..KN.....A.LDS...KS..........T....A..EK.INHR......L.E...........HKD....T.....PA.I...N..Q........AV... DHFR_allMis ..S...A.A............T............K........P..P....VL..................D...I......A.V.K...H.A.......V.LDS...K..I.L...........A..EK.INQR......LHE...............R....F...PA.....N..H........TV... DHFR_croPor ..S...A.A............T............K........P..P....VL..................D...I......A.V.K...H.A.......V.LDS...K..I.L...........A..EK.INQR......LHE...............R....F...PA.....N..H........TV... DHFR_chrPic ..RP....A..C......................K........TT......VL....R.............D...I........T.K...Y.AK......V.LDS...KS.I.....I....I..A..EK.V.QR......LHE................H.......PA.....N..R..Y.....TV... DHFR_anoCar ..L...S.A..C.........Q............K...K..M.PTQ.....V.....R.............D...II....M..V.K...Y.A....E..D.LQS...E..I.L...........A..DK.I.HR......LH..................I.S.A..PT.I...N..R.R.......I... DHFR_pytMol ..A..HS....CN......D.K............KH..K..M.TKE.....V..............H....N...V...K...DV.E...Y.AK..EE..D.L.TS.MER...K...........A..EK.I.HQ.......H.............K......K.T.IPT.I...N..Q.......NTI... DHFR_ambMex .GRI..A....CPS.....D.N....I.......K......M.ATQ.....V....R...L..............I......TA..P...Y.AN...G..E.L.TR...G...LL..I....L.R.LIGR.H.QR......LKE..C...L..F.....R........SLGI...N..Q........VEEA. DHFR_xenTro .NPF.HAV...CP.Q....E.S....L.......KH...L.M.PT..D.K.V....R..............E...I...K......T...Y..K..AE.ID.L.D...KD...L..VI....L.Q.L.EK.VNQRW.....L.E..C..YL.....NSFR.......ISPEL...N.VQ........IV... DHFR_xenLae .NQF.HAV...CP.Q....G.S....L.......KH...L.M.PT....K.V....R..............E...I...K......T...Y..K..TE.ID.L.D...KD...L...I....L.Q.L.EK.INQRW.....L.E..C..YL...N.NSFQ...Q...ISPEL...N.VQ........IV... DHFR_ranCat .NQF.HAV...CP.Q....G.T....L.......KH...L.M.PTT.....V....RR.................I......T...N...Y.AK...E.VH.LKT..MKE.I.LI..I....L...V.DK.VSQR..I...Q.E..C...L.DV..QS.........IS.EM...N..Q........ML... DHFR_latCha .ARL..S....CP.L....D.N...H.K.LS.......K....PT......V....R................V.V......Q.A.E...Y.A.D..S....L.S....DR..L...I...AL....LE..SQHR.....VL.Q..C..YL...NFN.FT....F...S.AI...S..Q.........S... DHFR_gadMor .SRV.......CPDA...YK.....H.T.LN...KH.R.L.V.PGA.D...V....R...N.........ND...I....Q.EA..S...H.A.D.PS..R.LDV..-GLLA.Q..LI....M.S.V.ET.CPRR......LKQ.P......D.SPAQFR........S.EL...N..Q.R.Q..QSV.P.. DHFR_tetNig .ARV..A....CPDL...R......H.I.LD...KH.RK..S.P..N....V....R.........H...AN...I....RSR...A...H.AHD.SS..Q.V.T-Q..EQA.Q..VI....L.Q.M.ER..TSR....HVLKQ.DC...L...RPQR.R...QF...PQEL......Q.RYQ...SLEP.. DHFR_hipHip .SRI..G....CPDL...NR.N...H.V.LS...KH.RS..A.P.EK....V....R.............NN...I....QC.A..A...H..ADFSS..R.VDT-...DQA.Q..VI....L...M.ES..TRR......LKQ.DC...L...SPD..RQ...F...PQELR..N..Q.R.....SIEP.. DHFR_solSen .SRV..G....CPD....MT.N...H.V.LS...KH.RT..A....K..K.V....R.............NN...I....QC.L..A...H.AADFSS..R.V.T-...DEA.QI..I....L...M.ES..IKR......LKQ..C...L...S.D..R....FA..SQEL...NA.Q.R.....SVEQ.. DHFR_oreNil .PRV..A....CPDR...NK.N...H.I.LSK..AH.RK..A.P..K....V........Y.........SN...I.....C.V..A...Y.ASDFSS..R.IDT-...DQA.Q..VI....L...M.GST.MRR....Q.LKQ..C...L...S.D..R....F.D.PQEL...N..Q.R.K...SIQE.. DHFR_dicLab .SRI..G....CPDL...M..N...H.V.LG...KH.RT..A.P..K....V....R.............NN...I...K.C.V..A...H.AADFNS..R.V.T-...DQA.Q..VI....L...L.ES..TRR......LKQ..C...L...CPD..Q....F...SQEL...N..Q.R.....STEQ.. DHFR_perFla .SRI..G....CPDL...N..N...H.V.LN...KH.RT..A.P..N....V....R.............NN...I.....C.A..A...H.A.DFSS..R.VDT-...QQA.T..VI....L...M.ES..TRR......LKQ..C...L...TPD..R....F...PQEL...N..Q.R.....SIQQ.. DHFR_spaAur .SRIV.G....CPDL...N..N...H.V.LNR..KH.RT..A.P..K....V....R.......D.F...NN...I.....C.V..A...H.AADFSS..S.VDT-...DQA.Q..VI....L...L.ESTRTSR......LKQ..C...L...SPD..S....F...PQGL...N..Q.......RIKQ.. DHFR_gasAcu .SRV..G....CPDL...CH.N...H...LS...KH.R...A.A..KD...V....R.............NN...I.......A..A...H.APDFSS..R.VDT-...EQA.Q..VI....L...LLES..TRR......LKQ..C...L...CPDR.R....F...PEEL...N.VQ.R.....SI.V.. DHFR_oryLat .TRT..G....CPDL....G.N...H...LSKD.AL.RK..S.PL.A.R..V....R.............QN...I....Q..A..A....VAADFAA..R.VDS-...ERA.Q...I....I.X.M.ES.APRR.I...VQKQ..C.......RP.QFR....F...SQEL...N.VS.......SIQQ.. DHFR_anoFim .SRV..A....CPDL...R......H.V.LN...KH.R...S.P.......V....R.............NN...I.......T..A...H.AQDFNS..R.VDM-K..EQ..Q..VI....L...L.ESQ.IRR....Q.LKQ..C...L.K.SPD..R....F...PQEL...N..Q.R.....SIEQ.. DHFR_esoLuc .SRV.......CP.....NK.N...H.K.LN...K...K..M.PF......VD...R........R.....N...I..........P...H.ASDFSS..R.LDTA.M.ER..Q..VI....L...M.ESE.IRR......L.Q......L...NQD..R....F...PQ.....S..Q.R.....SV.H.. DHFR_salSal .SRVP......CPDV...N..N...H.K.LN...K...K..M.........V....R........R.....N...I.......K..A...H.ASDFRS..C.LDMA.M.ERA.Q..VI....L...M.ESQ.TRR......L.Q.K....L...NQDQ.R....F...PQ.L...A.LQ.R.....SI.Q.. DHFR_oncMyk .SRV.......CPD....N..N...H.K.LN...K...K..M.........A....R........R.....N...I..........A...Q.ASDFRS..R.LDTA.M.ER..Q..VI....L.E.M.ESQ.TRR......L.Q.K....L...NQD..R....F...PQ.L...A.LQ.R.....SI.Q.. DHFR_danRer .SRI.......CPD.......N...H.I.LS..LKH..K..M.P.D...K.V....R.......AAH....N...I.......TA.E...Y.ASDFSS..H.LDSG..EKL..Q...I....L...V.ERS..RR......LKQ.DC...I.NF.MD.......F...PVGL..DN.VQ.L.....SIKH.. DHFR_cteIde .SRI.......CPD....RK.N...H.I.LS...KH..K..M.P.....K.V....R.......AQ.....N...I.......TA.E...Y.ASD.SS..H.LDSS..EKQ..Q...I...AL...V.ERS.PRR.....VLKQ.DC...I...NMD.......F...PTGL.D.N.VQ.V..CMIVS-... DHFR_cypCar .SRI..S....CPD.......N...H.I.LS...KH..K..M.PL....K.V.L..R.......AA.....N...I.......TA.E...Y.ASDFSS..H.LDSS..EKQ..Q...I...AL...V.ESS.PRR.....VLKQ.DC...I.D.NMD.......F...PTGL...N.VQ.V.....ST.H.. DHFR_ictPun .GRV.......CPD....R..N...H.I.LSK..KH..K..M.PT....K.V....R.......AQ.....N...V.......TA.E...Y.AADFSS..RIL.NA..EAQA.Q...I....L...V.ESSA.QR......LKQ.DC...I.N...D.FR....F...PAGL...N.LQ.V.....SVEQ.. DHFR_leuEri .TRLI.S....CP.....N..NF..H.I.LSK..KH.....S.P.....C.A....R...H..........N...I........V.E...Y.APD..S..A.LDSSD.QD...L...I...AL....LES.ASHR......L...D....I...N.N.F.....F.D.PA.I...N..RF.Y....RISPV. DHFR_squAca .PRLV......CP......D.NF..H.I.LSK.LKH..K..A.P.....R.A....R.........H....D.L.I........L.H..NY.AHD..S..AHLDSS..QD...L...I...TL.....ES.VSHRI.....LK.......L..M..N.F....KF.D.PTE...DN..Q.......RIAHA. DHFR_eptBur .QNPV.V.A..LP.....WK.G...HSKSLVK.MKH.T.L.SAAA-.....A.V..R...E.....F...RN...V....T.SHL.L...Y.ATDFSN..AQLSES..CCRI.HI..I....I....LL..ACHRI.I....H...A.....H..SKRFS.CQS...IPEEI...N.VQ.......VVAK.. DHFR_petMar -----------------------------..----------------....V....R.........K....N...I.......CL.E...Y.VPD.NS..S.IDS.AIQE.L.G...L..GG......E.SACRR..I..VL.TM.A.A...D..AD.F....--------------------------... homSap PWPPLR--NE TTSSVEGKQNL SIPEKNRP LTEQPELANKV AMNHPG KLLPEYPGVLSD xenTro PWPLLR--NE MTPTVEDKKNV SIPEKNRP LLEDPELKDKV LMEKPV RLLPEYPGISPE Identity: 36/55 (65%) Similar: 42/55 (76%) latCha PWHPKRLSNE TTPTVEGKQNV SIPEKNRP LLESPELADRV ALEHPS TLLPEFPGVSSA Identity: 40/57 (70%) Similar: 46/57 (80%) danRer PWHPIRLSNE MTPSDEGKKNV SIPAAHRP LLDSGELEKLV VMERSG KLLPEFPGVPVG Identity: 31/55 (56%) Similar: 37/55 (67%) esoLuc PWHPKRLNNE MTPFVEGKQNV SIPERNRP LLDTAEMAERV MMESEG RLLPEFPGVPQD salSal PWHPKRLNNE MTSSVEGKQNV SIPERNRP LLDMAEMAERA MMESQG RLLPEFPGVPQD oncMyk PWHPKRLNNE MTSSVEGKQNA SIPERNRP LLDTAEMAERV MMESQG RLLPEFPGVPQD cteIde PWHPIRLSNE MTPSVEGKKNV SIPAQNRP LLDSSELEKQV VMERSG KLLPEFPGVPTG cypCar PWHPIRLSNE MTPLVEGKKNV SIPAANRP LLDSSELEKQV VMESSG KLLPEFPGVPTG ictPun PWHPIRLSKE MTPTVEGKKNV SIPAQNRP ILENAELEAQA VMESSA RLLPEFPGVPAG gadMor PWHPTRLNNE VTPGAEDKQNV SIPEKNRP LLDVPE-GLLA VMETPC RLLPEYPGVSSE tetNig PWHPIRLDNE STPSVNGKQNV SIPEKHRP LVET-QLAEQA MMERPG RLLPQFPGVPQE hipHip PWHPVRLSNE ATPSEKGKQNV SIPEKNRP LVDT-ELADQA MMESPG RQLPEFPGVPQE solSen PWHPVRLSNE ATSSVKGKKNV SIPEKNRP LVET-ELADEA MMESPG RLLPEFAGVSQE oreNil PWHPIRLSKE ATPSVKGKQNV SIPEKNRP LIDT-ELADQA MMGSTG RLLPEFPDVPQE dicLab PWHPVRLGNE ATPSVKGKQNV SIPEKNRP LVET-ELADQA LMESPG QLLPEFPGVSQE perFla PWHPVRLNNE ATPSVNGKQNV SIPEKNRP LVDT-ELAQQA MMESPG RLLPEFPGVPQE spaAur PWHPVRLNRE ATPSVKGKQNV SIPDKFRP LVDT-ELADQA LMESTR SLLPEFPGVPQG gasAcu PWHPLRLSNE ATASVKDKQNV SIPEKNRP LVDT-ELAEQA LLESPG RLLPEFPGVPEE oryLat PWHPLRLSKD STPLVAGRQNV SIPEKNRP LVDS-ELAERA MMESPA RLLPEFPGVSQE anoFim PWHPVRLNNE STPSVEGKQNV SIPEKNRP LVDM-KLAEQV LMESQG RLLPEFPGVPQE leuEri PWHPIRLSKE STPSVEGKCNA SIPEKNRP LLDSSDLQDKV ALESPA KLLPEFPDVPAD squAca PWHPIRLSKE ATPSVEGKRNA SIPEKHRP HLDSSELQDKV AMESPV KLLPKFPDVPTE homSap PWPPLR--NE TTSSVEGKQNL SIPEKNRP LTEQPELANKV AMNHPG KLLPEYPGVLSD xenTro ...L..--.. M.PT..D.K.V ........ .L.D...KD.. L.EK.V R.......ISPE latCha ..H.K.LS.. ..PT......V ........ .L.S....DR. .LE..S T....F...S.A danRer ..H.I.LS.. M.P.D...K.V ...AAH.. .LDSG..EKL. V.ERS. .....F...PVG esoLuc ..H.K.LN.. M.PF......V ....R... .LDTA.M.ER. M.ESE. R....F...PQ. salSal ..H.K.LN.. M.........V ....R... .LDMA.M.ERA M.ESQ. R....F...PQ. oncMyk ..H.K.LN.. M.........A ....R... .LDTA.M.ER. M.ESQ. R....F...PQ. cteIde ..H.I.LS.. M.P.....K.V ...AQ... .LDSS..EKQ. V.ERS. .....F...PTG cypCar ..H.I.LS.. M.PL....K.V ...AA... .LDSS..EKQ. V.ESS. .....F...PTG ictPun ..H.I.LSK. M.PT....K.V ...AQ... IL.NA..EAQA V.ESSA R....F...PAG gadMor ..H.T.LN.. V.PGA.D...V ........ .LDV..-GLLA V.ET.C R........S.E tetNig ..H.I.LD.. S.P..N....V .....H.. .V.T-Q..EQA M.ER.. R...QF...PQE hipHip ..H.V.LS.. A.P.EK....V ........ .VDT-...DQA M.ES.. RQ...F...PQE solSen ..H.V.LS.. A....K..K.V ........ .V.T-...DEA M.ES.. R....FA..SQE oreNil ..H.I.LSK. A.P..K....V ........ .IDT-...DQA M.GST. R....F.D.PQE dicLab ..H.V.LG.. A.P..K....V ........ .V.T-...DQA L.ES.. Q....F...SQE perFla ..H.V.LN.. A.P..N....V ........ .VDT-...QQA M.ES.. R....F...PQE spaAur ..H.V.LNR. A.P..K....V ...D.F.. .VDT-...DQA L.ESTR S....F...PQG gasAcu ..H...LS.. A.A..KD...V ........ .VDT-...EQA LLES.. R....F...PEE oryLat ..H...LSKD S.PL.A.R..V ........ .VDS-...ERA M.ES.A R....F...SQE anoFim ..H.V.LN.. S.P.......V ........ .VDM-K..EQ. L.ESQ. R....F...PQE leuEri ..H.I.LSK. S.P.....C.A ........ .LDSSD.QD.. .LES.A .....F.D.PA. squAca ..H.I.LSK. A.P.....R.A .....H.. HLDSS..QD.. ..ES.V ....KF.D.PTE
Vertebrate reference sequences
>DHFR_homSap Homo sapiens (human) AC130896 tightly syntentic -+ MSH3 chr5 0 MVGSLNCIVAVSQNMGIGKNGDLPWPPLR 2 1 NEFRYFQRMTTTSSVE 1 2 GKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELK 2 1 EPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYK 0 0 EAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPE 2 1 YPGVLSDVQEEKGIKYKFEVYEKND* 0 >DHFR_panTro Pan troglodytes (chimp) XM_001138522 MVGSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELASKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND >DHFR_gorGor Gorilla gorilla (gorilla) CU104654 MVGSLNCIVAVSQNMGIGKNGVLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND >DHFR_ponAbe Pongo abelii (orangutan) XM_002815704 syntenic MVGSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND >DHFR_nomLeu Nomascus leucogenys (gibbon) XM_003261693 MVGSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNYPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEFPGVLSDVQEEKGIKYKFEVYEKND >DHFR_macMul Macaca mulatta (rhesus) XM_001110551 syntenic MVSSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLARSLDDALKLTEQPELANKVDMIWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFLPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND >DHFR_papAnu Papio anubis (baboon) FC175564 MARSLNCIVAVSQNMGIGKNGDLPWPPLRSEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLARSLDDALKLTEQPELANKVDMIWIVGGNSVYKEAMNHPGHLKLFVTRIMQDFESDTFLPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND >DHFR_calJac Callithrix jacchus (marmoset) XM_002745986 tightly syntentic -+ MSH3 4 recent pseudogenes MVQLLNCIVAVSQNMGIGKNGELPWPPLRNEFRYFQRMTTTSSVEDKQNLVIMGRKTWFSIPEKNRPLKDRINVVLSRELKEPPQGALLARSLDDALKLTEQPELANKVDMIWIIGGSSVYKEAMDHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPQYPGVPSDDQEEKGIKYKFEVYEKND >DHFR_saiBol Saimiri boliviensis (squirrel_monkey) AGCE01006205/AGCE01053749 numerous pseudogenes syntentic MSH3 MVQLLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINVVLSKELKEPPQGAHFLARSLDDALKLTEQPELANKVDMIWIIGGSSVYKEAMDHPGHLKLFVTRIMQDFESDTFFPEIDlkkykllseYPGVLSDVQEEKGIKYKFEVYEKND >DHFR_tarSyr Tarsius syrichta (tarsier) ABRT010238682/ABRT010238686/ABRT010468683 MVRPLYCIVAVSQNMGIGKDGTLPWPPLRNEFRYFQRMTTMSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKVPPPGAHFLAKSLDDALKLIEQPELANKVDKVWIVGGNSVYREAMSQPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLSEYPGVLSDVQEEKGIKYKFEVYEKND >DHFR_otogar Otolemur garnettii (bushbaby) AAQR03040271/AAQR03040268 MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFNYFQRMTKTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKESPQGAHFLAKSLDDALKLIEEPELANKVDMVWIVGGSSVYKEAMNQPGHVKLFVTRIMQDFECDTFFPEIDLKKYKLLSEYPGVLSDVQEEKGIKYKFEVYEKND >DHFR_micMur Microcebus murinus (lemur) wgs Blat MVRTLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQKMTTTSSVEGKQNLVIMGRKTWFSIPEKSRPLKDRINVVLSRELKEPPQGAHFVAKSLDDALKLIEQPELANKVDMVWIVGGSAVYKEAMNQPGHLKLFVTRIMQDFESDTFFPEIDLKKYKLLPE YPGVLSDVQEEKGIKYKFEVYEKND >DHFR_tupBel Tupaia belangeri (treeshrew) AAPY01231248/AAPY01231253/AAPY01729648/SRA|SRR005484.246484.2 MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVEGKQNIVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLAKSLDDALKLIEQPELANQVDMVWIVGGSSVYREAMNQPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPE >DHFR_musMus Mus musculus (mouse) NP_034179 F32W is methotrexate resistant MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQPELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEYPGVLSEVQEEKGIKYKFEVYEKKD >DHFR_ratNor Rattus norvegicus (rat) NM_130400 MVRPLNCIVAVSQNMGIGKNGDLPWPLLRNEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPQGAHFLAKSLDDALKLIEQPELASKVDMVWVVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLEKYKLLPEYPGVLSEIQEEKGIKYKFEVYEKKD >DHFR_criGri Cricetulus griseus (hamster) NM_001244016 MVRPLNCIVAVSQNMGIGKNGDFPWPMLRNEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPQGAHFLAKSLDDALKLIEQPELADKVDMVWIVGGSSVYKEAMNQPGHLRLFVTRIMQEFETDTFFPEIDLEKYKLLPEYPRVLPEVQEEKGIKYKFEVYEKKG >DHFR_perMan Peromyscus maniculatus (deermouse) GH506523 MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPQGAHFLAKSLDDALKLIEQPELANKVDMVWIVGGSSVYKEAMNKPGHLRLFVTRIMQEFESDTFFPDIDLEKYKLLPEYPGVLSEVQEEEGIKYKFEVYEKKA >DHFR_perPol Peromyscus polionotus (deermouse) GH462222 MVRPLNCIVAVSQNMGIGKNGDLPWPLLRNEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPQGAHFLAKSLDDALKLIEQPELANKVDMVWVVGGSSVYKEAMNKPGHLRLFVTRIMQEFESDTFFPDIDLEKYKLLPEYPGVLSEVQEEEGIKYKFEVYEKKA >DHFR_dipOrd Dipodomys ordii (kangaroo_rat) wgsBlast MGRSLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSTVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPQGAHFLAKSLDEALKLIEQPELNNKVDMVWIVGGSSVYKEAMNQPGHHKLFVTRIMQEFESDTFFPEIDLEKYKLLPEYPGVLSEVQEEKGIKYKFEVYEKID >DHFR_speTri Spermophilus tridecemlineatus (ground_squirrel) ti|1005446275 MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMMTTPSVEGKQNLVIIGRKTWFSIPEKNRPLNDRINIVLSRELKELPKGAHFLAKSLTDALKLTEQPELASKIDMIWIVGGSSVYKEAMIQPGHIKLFVTRIMQEFESDTFFPEIDLEKYKLLPEYPGILSEVQEEKGIKYKFEVYEKTD >DHFR_cavPor Cavia porcellus (guinea_pig) XM_003479518 Hystricognathi MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFGYFQRMTTTPSVEGKQNLVIMGRKTWFSLPEKSRPLKDRINVVLSRELKEPPQGAHFLAPSLDDALKLIEQPGLANEVDMVWIVGGSSVYKEAMERPGHIKLFVTRIMQEFESDTFFPEIDLKKYRLLPEYPGVPSEIQEEKGIKYKFEVYEKND >DHFR_oryCun Oryctolagus cuniculus (rabbit) FJ687582 MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINVVLSRELKEPPQGAHFLAKSLADALKLIEEPELAEKVDMAWIVGGSSVHKEAMNQPGHLRLFVTRIIQEFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND >DHFR_ochPri Ochotona princeps (pika) wgsBlast/ti|1497151195 MVRPLNCIVAVSQNMGIGRNGDLPWPPLRNEFKYFQRMTTTSTVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPPGAHFLAKSLDDALKLIEEPELAERVDMVWIVGGSSVYKEAMDKPGHLRLFVTRIMQEFESDTFFPEIDLEKYKLLPEYPGILPEVQEERGIKYRFEVYEKND >DHFR_felCat Felis catus (cat) wgsBlast MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRDLKEPPQGAHFLAKSLDDALELTEQPELAHKVDMVWVVGGSSVYKEAMNRPGHVRLFVTRIMQEFESDTFFPEIDLEKYKLLPEYPGVLSDIQEEKSIKYKFEVYEKNN >DHFR_canFam Canis familiarus (dog) XM_003432480 MVRTLNCIVAVSQNMGIGRNGDLPWPPLRNEFKYFQRMTTNSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRDLKEPPQGAHFLAKSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNKPGHLRLFVTRIMHEFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND >DHFR_vulVul Vulpes vulpes (fox) SRA|SRR094997.876315.2 MVRTLNCIVAVSQNMGIGRNGTVPWPPLRNEFKYFQRMTTNSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRDLKEPPQGAHFLAKSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNKPGHLRLFVTRIMHEFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND >DHFR_musPut Mustela putorius (ferret) AEYP01048652/AEYP01048654/AEYP01048651/SRA|SRR071690.159842.2 MVRTLNCIAAVSQNMGIGKNGDLPWPPLRNEFKFFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRDLKEPPQGAHFLAKSLDDALKLIEQPELANKVDMVWIVGGSSVYKEAMNKPGHLRLFVTRIMQEFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEknd >DHFR_ailMel Ailuropoda melanoleuca (panda) XM_002916218 MVRTLNCIAAVSQNMGIGKNGDLPWPPLRNEFKFFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRDLKEPPQGAHFLAKSLDDALKLIEQPELANKVDIVWIVGGSSVYKEAMNKPGHLRLFVTRIMQEFESDTFFPEIDLDKYKLLPEYPGVPSDVQEEKGIKYKFEVYEKND >DHFR_ursAme Ursus americanus (bear) GW292063 fragment MVRTLNCIAAVSQNMGIGKNGDLPWPPLRNEFKFFQRMTTTSSVEGKQNLVIMGRKT >DHFR_equCab Equus caballus (horse) XM_001504643 MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTASSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLAKSLDDALSLLEQPELANKVDMVWIVGGSSVYKEAMNKPGHLRLFVTRIMQEFESDTFFPEIDFEKYKLLPEYPGVLSDIQEEKGIKYKFEVYEKND >DHFR_cerSim Ceratotherium simum (rhino) AKZM01005342 MMRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLAKSLEDALSLIEQPELTNKVDMVWIVGGSSVYKEAMNKPGHLRLFVTRIMQEFESDTFFPEIDFKKYKLLPEYPGVLSDIQEEKGIKYKFEVYEKND >DHFR_vicPac Vicugna paca (vicuna) wgsBlast MVRPLDCIIAVSQNMDMGKNGDLPWPplrNEFKYFQRMTTASSAEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLANSLDDALKVIEQPELTNKVDMVWIVGGSSVYKEAMDKPGHLRLFVTRIMQEFESDTFFPEIDLEKYKLLSEYPGVPSDVQEEKGIKYKFEVYEKNN >DHFR_susScr Sus scrofa (pig) NM_001244064 MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEYKYfQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPQGAHFLAKSLDDALKLTEQPELKNKVDMVWIVGGSSVYKEAMNKPGHIRLFVTRIMKEFESDTFFPEIDLEKYKLLSECSGVPSDVQEEKGIKYKFEVYEKNN >DHFR_turTru Tursiops truncatus (dolphin) DT661408 MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEYRYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPQGAHFLAKSLDGALKLIEQPELTNTVDMVWIVGGGSVYKEAMNKPGHLRLFVTRIMQEFESDTFFPEIDLEKYKLIPEYPGVPSDVQEEKGIKYKFEVYEKNN >DHFR_oviAri Ovis aries (sheep) GO728717 MVRPLNCIVAVSQNMGIGKNGNLPWPPLRNEFKYFQRMTTVSSEEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPKGAHFLAKSLDDALELIEDPELTNKVDMVWIVGGSSVYKEAMNKPGHLRLFVTRIMQEFESDVFFPEIDFEKYKLLPEYPGVPLDVQEEKGIKYKFEVYEKNN >DHFR_capHir Capra hircus (goat) JO612108 MVRPLNCIVAVSQNMGIGKNGNLPWPPLRNEFKYFQRMTTVSSEEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPKGAHFLAKSLDDALELIEDPELTNKVDMVWIVGGSSVYKEAMNKPGHLRLFVTRIMQEFESDVFFPEIDFEKYKLLPEYPGVPSDVQEEKGIKYKFEVYEKNN >DHFR_bosTau Bos taurus (cow) NM_001077883 MVRPLNCIVAVSQNMGIGKNGNLPWPPLRNEFQYFQRMTTVSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPKGAHFLAKSLDDALELIEDPELTNKVDVVWIVGGSSVYKEAMNKPGHVRLFVTRIMQEFESDAFFPEIDFEKYKLLPEYPGVPLDVQEEKGIKYKFEVYEKNN >DHFR_myoLuc Myotis lucifugus (microbat) wgsBlast MVRTLNCIAAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPKKNRPLKDRINLVLSRELKEPPKGAHFLAKSLDDALKLIEEPELTNQVDMVWIVGGSSVYKEAMSKPGHQRLFVTRIMQEFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKNE >DHFR_pteVam Pteropus vampyrus (macrobat) wgsBlast MVLSLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSveGKRNLVIMGRKTWFSIPKKNRPLKDRINLVLSRELKEPPKGAHFLAKSLDDALKLIEQPELRSKVDMVWIVGGSSVYKEAMNKPGHIKLFVTKIMQEFESDIFFPEIDLGKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND >DHFR_eptFus Eptesicus fuscus (brown bat) ALEH01016635 ALEH01087690 ALEH01016633 MVRTLNCIAAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTTSAEGKQNLVIMGRKTWFSIPEKSRPLKDRINLVLSRELKEPPKGAHFLAKSLDDALKLIEEPELTNKVDMVWIVGGSSVYKEAMSKPGHQRLFVTRIMQEFESDTFFPEIDTEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKNE >DHFR_eriEur Erinaceus europaeus (hedgehog) wgsBlast/ti|909141827 MVRPLNCMVAVSQNMGIGKNGELPWPPLRNEFKHFRTMTSTPSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLAKSLDAALKLIDQPELTNKVDRV----------EVMNRPGHIRLFVTRILQEFECDTFFPEIDLKNYRLLP-------------------------- >DHFR_sorAra Sorex araneus (shrew) wgsBlast MVRPLNCIVAVSQNMGIGKNGELPWPPLRNEFTYFRKMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPTGAHFLAKSLDDALKLIEQPELTNKVDMVWIVGGSSVYQEVMNKPGHLRLFVTRIMQEFECDTFFPEIDLEKYKLLTDYPGVVSDVQEEKGIKYKFEVYEKKD >DHFR_eriEur Erinaceus europaeus (hedgehog) AMDU01007281 AMDU01007279 AANN01114614 AANN01744600 AMDU01007278 MVRPLNCMVAVSQNMGIGKNGELPWPPLRNEFKHFRTMTSTPSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLAKSLDDALKLIDQPELTNKVDRVWIIGGSSVYKEVMNRPGHIRLFVTRILQEFECDTFFPEIDLKNYRLLPEYPGVLSDIQEEKGIKYKFEVYERND >DHFR_conCri Condylura cristata (starnosed mole) AJFV01021063 MVRPLNCIVAVSQNMGIGKNGDLPWPSLRNEYSYFRRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINVVLSRELKEPPQGAHFLAKSLDDALKLIEQPELTNKVDMVWIVGGSSVYKEAINKSGHIRLFVTRIMQEFESDTFFPEIDLKKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND >DHFR_loxAfr Loxodonta africana (elephant) XM_003408117 error single trace TTTTTSSV MVRPLNCIVAVSQNMGIGKNGDLPwPplRNEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRGLKEPPQGAHFLAKSLDDALKLIEQPELRNKVDMVWIVGGSSVYKEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLEKYKRLSEYPGVLSDVQEEKGIKYKFEVYEKKD >DHFR_triMan Trichechus manatus (manatee) AHIN01038459 AHIN01038460 MVRRLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTTTSSEEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLAKSLDDALKLIEQPELENKVDMVWIVGGSSVYKEAMNHPGHLRLFVTRIMQEFESDTFFPEIDFKKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND >DHFR_proCap Procavia capensis (hyrax) wgsBlast MVRPLSCIVAVSQNMGIGKNGDLPWPPLRNEFKHFRTMTSTPSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLAKSLDDALKLIEQPELTNKVDLVWIIGGSSVYKEAMSQPGHLTLFVTRIMQEFESDTFFPEIDLEKFKLLPEYPGVPSDVQEEKGIKYKFEVYEKKD >DHFR_eleEdw Elephantulus edwardii (elephant shrew) AMGZ01128973 AMGZ01128972 AMGZ01128971 MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPQGAHFLAKSLDEALKLIEQPELRNKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLQKYKLLPKYPGVASDIQEEKGIKYKFEVYEKKD >DHFR_chrAsi Chrysochloris asiatica (golden mole) AMDV01216092 AMDV01216090 AMDV01216089 MVRPINCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHLLARSLDDALKLIEQPELGNKVDIVWIVGGSSVYKDAMNQPGHVRLFVTRIMQEFESDTFFPEIDLEKYRLLPEYPGVLSAVQEEKGIKYKFEVYEKND >DHFR_oryAfe Orycteropus afer (aardvark) ALYB01132696 ALYB01132694 ALYB01132692 MVRQLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTTSSSVDGKQNVVIMGRKTWFSIPKKHRPLKDRINLVLSRELKEPPQGAHFLAKSLDDALKLIEQPEIGNKVEMVWIIGGSSVYKEAMDQPGHLRLFVTRIMQEFESDTFFPEIDLQKYKLLPEYPGVLSDVQEENGIKYKFEVYEKNE >DHFR_dasNov Dasypus novemcinctus (armadillo) AAGV03076267 AAGV03076265 MVRPLNCIVAVSQNMGIGKNGDMPWPPLRNEYKYFQKMTTVSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELREPPQGAHFLAKSLDDALKLIEQPELADKVDMIWIVGGSSVYKEAMNQPGHIRLFVTRIMQEFECDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND >DHFR_choHof Choloepus hoffmanni (sloth) ABVD01033446 ABVD01033450 MVRPLNCIVAVSQNMGIGKNGDLPWPLLRNEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLAKSLDDALKLIEQPELANKVDMVWIVGGSSVYKEAMNRPGHLRLFVTRIMQEFESDTFFPEIDTEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND >DHFR_monDom Monodelphis domestica (opossum) XM_001363581 MVRQLNCIAAVSKNMGIGKDGDLPWPLLRNEFKYFQKMTTTPSVEGKQNLVIMGKKTWFSIPEKCRPLKDRINVVLSRELKEPPQGAHYLASSLDNALNLLELPELADKVDMVWIVGGSSVYKEAMNKPGHQRLFITRILQEFESDTFFPEIDLKKYKQLQDYPGVSPDVQEEKGIQYKFEVYEKHE >DHFR_macEug Macropus eugenii (wallaby) wgsBlast MAPTINCIVAVAQNMGMGKNGDLPWPPLRKEFQYFQKMTTTPSVEGKQNLLIMGKKTWFSIPEKNRPLKDRINLVLSRELKDPPLGAHYLAKSLDDALDLLRLPELANTVDMVWVVGGSSVYKEAMNKPGHQRLFVTRILQEFESDTFFPEIDLKRYKLLPEYPGVLTDVQEEKGIQHKCEIHEKHD >DHFR_sarHar Sarcophilus harrisii (tasmanian_devil) AFEY01286966/AFEY01033989/AFEY01359230 MVRPINCIAAVSQNMGIGKNGDLPWPPLRNEFNYFQKMTTTSSVEGKQNLLIMGKKTWFSIPEKHRPLKDRINLVLSRELKEPPPGAHYLAKSLDDALDLLELPELADKVDMIWVIGGSSVYKEAMEKPGHQRLFVTRILQEFECDTFFPEIDLKKYKLLPEYPGVLTDIQEEKGIQYKFEVYEKHD >DHFR_triVul Trichosurus vulpecula (brushtail) EG594753 MVRTINCIVAVSQNMGIGKNGDLPWPPLRNDFKHFQKMTTIPSVEGKQNLLITGKKTWFSIPEKSRPLKDRINVVLSRELKEPPPGAHYLAKSLDDALDLLELPELANKVDMIWVVGGSSVYKEAMNKRGHNRLFVTRILQEFESDTFFPEIDLKRYKLLXEYPGVLTDV >DHFR_ornAna Ornithorhynchus anatinus (platypus) XM_001512186 MGPLLNCIVAVAKNGGIGNKGDLPWPALRNEFRYFQKMTTTPTVKGKQNLVIMGKKTWFSIPEKSRPLKDRINIVLSRELKEPPQGAHYVAKSLDDALNLLHLPELASKVDMVWIIGGSSVYKEAMEKGGHQRLFVTRILQDFESDTFFPEIDLEKYKLIPEYPGVPADTQEEKGIQYRFEVYEKHI >DHFR_tacAcu Tachyglossus aculeatus (echidna) SRA:EUGXWLM02GHH9L/EUPZL4S02GL94B MGRPLNCIAAVAKNGGIGNKGDLPWPPLRNEYRYFQKMTTTPTVEGKENLVIMGKKTWFSIPEKSRPLKDRINIVLSRELKEPPQGAHYVAKSLDDALNLLNLPELASKVDMVWIIGGSSVYKEAMEKPGHQRLFVTRILQEFESDTFFPEIDLEKYKLIPEYPGVPADTQEEKGIQYRFEVYEKHI >DHFR_galGal Gallus gallus (chicken) NM_001006584 MVRSLNSIVAVCQNMGIGKDGNLPWPPLRNEYKYFQRMTSTSHVEGKQNAVIMGKKTWFSIPEKNRPLKDRINIVLSRELKEAPKGAHYLSKSLDDALALLDSPELKSKVDMVWIVGGTAVYKAAMEKPINHRLFVTRILHEFESDTFFPEIDYKDFKLLTEYPGVPADIQEENGIQYKFEVYQKSVLAQ >DHFR_melGal Meleagris gallopavo (turkey) genome/blat ------------QNMGIGKDGNLPWPPLRNEYKYFQRMTSTSHVE------------------------------------EAPKGAHYLSKSLDDALALLDSPELKSKVDMVWIVGGTSVYKAAMEKPINHRLFVTRILHEFESDTFFPEIDYKDFKLLKEYPGVPADVQEENGIQYKFEVYQKSVLAQ >DHFR_lagLag Lagopus lagopus (ptarmigan) GW704257 MVRSLNSIVAVCQNMGIGKDGNLPWPPLRNEYKYFQRMTSTSHVEGKQNALIMGKKTWFSIPEKNRPLKDRINIVLSRELKEAPEGAHYLSKSLDDALALLDSPELKSKVDMVWIVGGTSVYKAAMEKPINHRLFVTRILHEFESDTFFPEIDYKDFKLLTEYPGVPADVQEENGIQYKFEVYQKSVLAQ >DHFR_anaPla Anas platyrhynchos (duck) scaffold675 MVRSLNSIVAVCQNMGIGKDGNLPWPPLRNEYKYFQRMTSTSHVEGKQNALIMGKKTWFSIPEKNRPLKDRINIVLSRELKEAPEGAHYLSKSLDDALALLDSPELKSKVDMVWIVGGTSVYKAAMEKPINHRLFVTRILHEFESDTFFPEIDYKDFKLLTEYPGVPADVQEENGIQYKFEVYEKTVLAQ >DHFR_taegut Taeniopygia guttata (finch) XM_002190440 MPRSLNSIVAISQNMGIGKDGRLPWPPLRNDYKYFQRMTSASPVEGKKNAVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEAPKGAHYLSKSLDDALALLDSPELQSKVCMVWIVGGSSVYKEAMEKPIHHRLFVTRILKEFESDTFFPEIDHKDYKLLTEYPGVPADIQEENGIQYKFEVYEKTVVP >DHFR_ficHyp Ficedula hypoleuca (flycatcher) SRA|SRR029160.210264.2 MVRSLNSIVAVSQNMGIGKDGRLPWPPLRNEYKYFQRMTSTPRVEGKQNALIMGKKTWFSIPEKNRPLKDRINIVLSRELKEAPEGAHYLSKSLDDALALLDSPELKSKVDMVWIVGGTSVYKAAMEKPIHHRLFVTRILKEFESDTFFPEIDHKDYKLLTEYPGVPADIQEENGIQYKFEVYEKAVV* >DHFR_melUnd Melopsittacus undulatus (budgerigar) SRA|SRR029329.119695.2 MVRSLNSIVAVCQNMGIGKDGSLPWPPFRNEYKYFQRMTSTSQVEGKQNALIMGKKTWFSIPEKNRPLKDRINIVLSRELQETPKGAHYLSKNLDDALALLDSPELKSKVDMVWIVGGTSVYKAAMEKPINHRLFVTRILQEFESDTF >DHFR_allMis Alligator mississippiensis (alligator) genome/blat MVSSLNAIAAVSQNMGIGKNGTLPWPPLRNEFKYFQRMTTTPSVPGKQNVLIMGKKTWFSIPEKNRPLKDRINIVLSRELAEVPKGAHHLARSLDDALVLLDSPELKNKIDLVWIVGGSSVYKAAMEKPINQRLFVTRILHEFESDTFFPEIDLEKYRLLPEFPGVPADVQEENGIHYKFEVYEKT >DHFR_croPor Crocodylus porosus (crocodile) genome/blat composite seq with alligator MVSSLNAIAAVSQNMGIGKNGTLPWPPLRNEFKYFQRMTTTPSVPGKQNVLIMGKKTWFSIPEKNRPLKDRINIVLSRELAEVPKGAHHLARSLDDALVLLDSPELKNKIDLVWIVGGSSVYKAAMEKPINQRLFVTRILHEFESDTFFPEIDLEKYRLLPEFPGVPADVQEENGIHYKFEVYEKTV >DHFR_chrPic Chrysemys picta bellii (turtle) AHGY01047456 AHGY01047458 MVRPLNCIAAVCQNMGIGKNGDLPWPPLRNEFKYFQRMTTTTTVEGKQNVLIMGKRTWFSIPEKNRPLKDRINIVLSRELKETPKGAHYLAKSLDDALVLLDSPELKSKIDMVWIIGGSSIYKAAMEKPVHQRLFVTRILHEFESDTFFPEIDLEKYKHLPEYPGVPADVQEENGIRYKYEVYEKTV* >DHFR_anoCar Anolis carolinensis (lizard) genome/blat XM_003216272 MVLSLNSIAAVCQNMGIGKNGQLPWPPLR 2NEFKYFQKMTMTPTQEGKQNVVIMGKRTWFSIPEKNRPLKDRINIILSREMKEVPKGAHYLARSLDEALDLLQSPELENKIDLVWIVGGSSVYK AAMDKPIHHRLFVTRILHDFESDTFFPEIDLEKYKLIPSYAGVPTDIQEENGIRYRFEVYEKNI >DHFR_pytMol Python molurus (python) AEQU011279324/AEQU010134262/AEQU010204446/AEQU010142865/AEQU010435622/AEQU010656196 MVASLHSIVAVCNNMGIGKDGKLPWPPLRNEFKHFQKMTMTTKEEGKQNVVIMGKKTWFSIPEKHRPLKNRINVVLSKELKDVPEGAHYLAKSLEEALDLLETSEMERKVDKVWIVGGSSVYK AAMEKPIHHQLFVTRIMHDFESDTFFPEIDLKKYKLLPKYTGIPTDIQEENGIQYKFEVYENTI >DHFR_ambMex Ambystoma mexicanum (axolotl) CN050864 MGRILNAIVAVCPSMGIGKDGNLPWPILRNEFKYFQRMTMTATQEGKQNVVIMGRKTWLSIPEKNRPLKGRINIVLSRELTAPPPGAHYLANSLDGALELLETRELAGKVDLLWIIGGSSLYRELIGRPHHQRLFVTRILKEFECDTFLPEFDLEKYRLLPEYPGVSLGIQEENGIQYKFEVYEKVEEA >DHFR_xenTro Xenopus tropicalis (frog) CT025223 MRNPFLHAVVAVCPPNQGIGKEGSLPWPLLRNEFKHFQRLTMTPTVEDKKNVVIMGRKTWFSIPEKNRPLKERINIVLSKELKEPPTGAHYLSKSLAEAIDLLEDPELKDKVDLVWVIGGSSLYQELMEKPVNQRWFVTRILQEFECDTYLPEIDLNSFRLLPEYPGISPELQEENGVQYKFEVYEKIV >DHFR_xenLae Xenopus laevis (frog) NM_001095037 MRNQFLHAVVAVCPPNQGIGKGGSLPWPLLRNEFKHFQRLTMTPTVEGKKNVVIMGRKTWFSIPEKNRPLKERINIVLSKELKEPPTGAHYLSKSLTEAIDLLEDPELKDKVDLVWIIGGSSLYQELMEKPINQRWFVTRILQEFECDTYLPEINLNSFQLLPQYPGISPELQEENGVQYKFEVYEKIV >DHFR_ranCat Rana catesbeiana (bullfrog) GO463031 ---------------------GGTLPWPLLRNEFKHFQRLTMTPTTEGKQNVVIMGRRTWFSIPEKNRPLKGRINIVLSRELTEPPNGAHYLAKSLDEAVHLLKTPEMKEKIDLIWIIGGSSLYKEVMDKPVSQRLFITRIQQEFECDTFLPDVDLQSYKLLPEYPGISSEMQEENGIQYKFEVYEKML >DHFR_latCha Latimeria chalumnae (coelocanth) AFYH01150805/AFYH01150806/AFYH01150807/AFYH01150815/AFYH01150814 MGAARLLNSIVAVCPNLGIGKDGNLPWHPKRLSNEFRYFQKMTTTPTVEGKQNVVIMGRKTWFSIPEKNRPLKGRVNVVLSRELQEAPEGAHYLARDLDSALKLLESPELADRVDLVWIIGGSALYKEALEHPSQHRLFVTRVLQQFECDTYLPEINFNKFTLLPEFPGVSSAIQEESGIQYKFEVYEKNS >DHFR_lepOcu Lepisosteus oculatus (spotted_gar) AHAT01012362 0 MPRPINCIVAVCPNMGIGHNGNLPWHPKRLS 2 1 NEFKYFQKMTMTPTLE 1 2 GQQNAVIMGRKTWFSIPERNRPLKNRINIVLSRELK 2 1 NPPEGAHYLASDFSSALRLLDSAPQTDQVDQVWVIGGSSVYK 0 0 EAMEAPGWCRLFVTQILQDFECDTFLPEINLNKYRLLPE 2 1 FPGVPLEVQEENGIRYKFEVYESIQN* 0 >DHFR_gadMor Gadus morhua (cod) FF410604 novel insertion 3 transcripts MSRVLNCIVAVCPDAGIGYKGDLPWHPTRLNNEFKHFRRLTVTrtPGAEDKQNVVIMGRKTWNSIPEKNRPLNDRINIVLSRQLEAPPSGAHHLARDLPSALRLLDVPEGLLADQVWLIGGSSMYSEVMETPCPRRLFVTRILKQFPSDTFFPDISPAQFRLLPEYPGVSSELQEENGIQYRFQVYQSVDP >DHFR_tetNig Tetraodon nigroviridis (pufferfish) CR710339 MARVLNAIVAVCPDLGIGRNGDLPWHPIRLDNEFKHFRKMTSTPSVNGKQNVVIMGRKTWFSIPEKHRPLANRINIVLSRRSREPPAGAHHLAHDLSSALQLVETQLAEQADQVWVIGGSSLYQEMMERPGTSRLFVTHVLKQFDCDTFLPEIRPQRYRLLPQFPGVPQELQEEKGIQYRYQVYESLEP >DHFR_hipHip Hippoglossus hippoglossus (halibut) EB031730 MSRILNGIVAVCPDLGIGNRGNLPWHPVRLSNEFKHFRSMTATPSEKGKQNVVIMGRKTWFSIPEKNRPLNNRINIVLSRQCKAPPAGAHHLSADFSSALRLVDTELADQADQVWVIGGSSLYKEMMESPGTRRLFVTRILKQFDCDTFLPEISPDKYRQLPEFPGVPQELREENGIQYRFEVYESIEP >DHFR_solSen Solea senegalensis (sole) FF286406 MSRVLNGIVAVCPDMGIGMTGNLPWHPVRLSNEFKHFRTMTATSSVKGKKNVVIMGRKTWFSIPEKNRPLNNRINIVLSRQCKLPPAGAHHLAADFSSALRLVETELADEADQIWIIGGSSLYKEMMESPGIKRLFVTRILKQFECDTFLPEISLDKYRLLPEFAGVSQELQEENAIQYRFEVYESVEQ >DHFR_oreNil Oreochromis niloticus (tilapia) GR620119 MPRVLNAIVAVCPDRGIGNKGNLPWHPIRLSKEFAHFRKMTATPSVKGKQNVVIMGKKTWYSIPEKNRPLSNRINIVLSRECKVPPAGAHYLASDFSSALRLIDTELADQADQVWVIGGSSLYKEMMGSTGMRRLFVTQILKQFECDTFLPEISLDKYRLLPEFPDVPQELQEENGIQYRFKVYESIQE >DHFR_dicLab Dicentrarchus labrax (seabass) FM022259 mSRILNGIVAVCPDLGIGMNGNLPWHPVRLGNEFKHFRTMTATPSVKGKQNVVIMGRKTWFSIPEKNRPLNNRINIVLSKECKVPPAGAHHLAADFNSALRLVETELADQADQVWVIGGSSLYKELMESPGTRRLFVTRILKQFECDTFLPEICPDKYQLLPEFPGVSQELQEENGIQYRFEVYESTEQ >DHFR_perFla Perca flavescens (perch) GO655828 MSRILNGIVAVCPDLGIGNNGNLPWHPVRLNNEFKHFRTMTATPSVNGKQNVVIMGRKTWFSIPEKNRPLNNRINIVLSRECKAPPAGAHHLARDFSSALRLVDTELAQQADTVWVIGGSSLYKEMMESPGTRRLFVTRILKQFECDTFLPEITPDKYRLLPEFPGVPQELQEENGIQYRFEVYESIQQ >DHFR_spaAur Sparus aurata (seabream) FM155123 MSRIVNGIVAVCPDLGIGNNGNLPWHPVRLNREFKHFRTMTATPSVKGKQNVVIMGRKTWFSIPDKFRPLNNRINIVLSRECKVPPAGAHHLAADFSSALSLVDTELADQADQVWVIGGSSLYKELMESTRTSRLFVTRILKQFECDTFLPEISPDKYSLLPEFPGVPQGLQEENGIQYKFEVYERIKQ >DHFR_gasAcu Gasterosteus aculeatus (stickleback) ENSGACT00000004939 MSRVLNGIVAVCPDLGIGCHGNLPWHPLRLSNEFKHFRRMTATASVKDKQNVVIMGRKTWFSIPEKNRPLNNRINIVLSRELKAPPAGAHHLAPDFSSALRLVDTELAEQADQVWVIGGSSLYKELLESPGTRRLFVTRILKQFECDTFLPEICPDRYRLLPEFPGVPEELQEENGVQYRFEVYESIDV >DHFR_oryLat Oryzias latipes (medaka) BJ496706 MTRTLNGIVAVCPDLGIGKGGNLPWHPLRLSKDFALFRKMTSTPLVAGRQNVVIMGRKTWFSIPEKNRPLQNRINIVLSRQLKAPPAGAHFVAADFAAALRLVDSELAERADQVWIIGGSSIYXEMMESPAPRRLIVTRVQKQFECDTFFPEIRPEQFRLLPEFPGVSQELQEENGVSYKFEVYESIQQ >DHFR_anoFim Anoplopoma fimbria (sablefish) BT082890 MSRVLNAIVAVCPDLGIGRNGDLPWHPVRLNNEFKHFRRMTSTPSVEGKQNVVIMGRKTWFSIPEKNRPLNNRINIVLSRELKTPPAGAHHLAQDFNSALRLVDMKLAEQVDQVWVIGGSSLYKELMESQGIRRLFVTQILKQFECDTFLPKISPDKYRLLPEFPGVPQELQEENGIQYRFEVYESIEQ >DHFR_esoLuc Esox lucius (pike) BT079933 MSRVLNCIVAVCPNMGIGNKGNLPWHPKRLNNEFKYFQKMTMTPFVEGKQNVDIMGRKTWFSIPERNRPLKNRINIVLSRELKEPPPGAHHLASDFSSALRLLDTAEMAERVDQVWVIGGSSLYKEMMESEGIRRLFVTRILQQFESDTFLPEINQDKYRLLPEFPGVPQDVQEESGIQYRFEVYESVDH >DHFR_salSal Salmo salar (salmon) BT047529 MSRVPNCIVAVCPDVGIGNNGNLPWHPKRLNNEFKYFQKMTMTSSVEGKQNVVIMGRKTWFSIPERNRPLKNRINIVLSRELKKPPAGAHHLASDFRSALCLLDMAEMAERADQVWVIGGSSLYKEMMESQGTRRLFVTRILQQFKSDTFLPEINQDQYRLLPEFPGVPQDLQEEAGLQYRFEVYESIDQ >DHFR_oncMyk Oncorhynchus mykiss (trout) EZ786723 MSRVLNCIVAVCPDMGIGNNGNLPWHPKRLNNEFKYFQKMTMTSSVEGKQNAVIMGRKTWFSIPERNRPLKNRINIVLSRELKEPPAGAHQLASDFRSALRLLDTAEMAERVDQVWVIGGSSLYEEMMESQGTRRLFVTRILQQFKSDTFLPEINQDKYRLLPEFPGVPQDLQEEAGLQYRFEVYESIDQ >DHFR_danRer Danio rerio (zebrafish) NM_131775 genome duplicate BC165261 MSRILNCIVAVCPDMGIGKNGNLPWHPIRLSNELKHFQKMTMTPSDEGKKNVVIMGRKTWFSIPAAHRPLKNRINIVLSRELKTAPEGAHYLASDFSSALHLLDSGELEKLVDQVWIIGGSSLYKEVMERSGHRRLFVTRILKQFDCDTFIPNFDMDKYKLLPEFPGVPVGLQEDNGVQYLFEVYESIKH >DHFR_cteIde Ctenopharyngodon idella (carp) CK233011 MSRILNCIVAVCPDMGIGRKGNLPWHPIRLSNEFKHFQKMTMTPSVEGKKNVVIMGRKTWFSIPAQNRPLKNRINIVLSRELKTAPEGAHYLASDLSSALHLLDSSELEKQVDQVWIIGGSALYKEVMERSGPRRLFVTRVLKQFDCDTFIPEINMDKYKLLPEFPGVPTGLQDENGVQYVFECMIVS >DHFR_cypCar Cyprinus carpio (carp) CA968873 MSRILNSIVAVCPDMGIGKNGNLPWHPIRLSNEFKHFQKMTMTPLVEGKKNVVLMGRKTWFSIPAANRPLKNRINIVLSRELKTAPEGAHYLASDFSSALHLLDSSELEKQVDQVWIIGGSALYKEVMESSGPRRLFVTRVLKQFDCDTFIPDINMDKYKLLPEFPGVPTGLQEENGVQYVFEVYESTDH >DHFR_ictPun Ictalurus punctatus (catfish) FD330522 MGRVLNCIVAVCPDMGIGRNGNLPWHPIRLSKEFKHFQKMTMTPTVEGKKNVVIMGRKTWFSIPAQNRPLKNRINVVLSRELKTAPEGAHYLAADFSSALRILENAELEAQADQVWIIGGSSLYKEVMESSAHQRLFVTRILKQFDCDTFIPNIDLDKFRLLPEFPGVPAGLQEENGLQYVFEVYESVEQ >DHFR_leuEri Leucoraja erinacea (skate) EE989413 MTRLINSIVAVCPNMGIGNNGNFPWHPIRLSKEFKHFQRMTSTPSVEGKCNAVIMGRKTWHSIPEKNRPLKNRINIVLSRELKEVPEGAHYLAPDLDSALALLDSSDLQDKVDLVWIIGGSALYKEALESPASHRLFVTRILQDFDSDTFIPEINLNKFKLLPEFPDVPADIQEENGIRFKYEVYERISPV >DHFR_squAca Squalus acanthias (dogfish) DV497998 mPRLVNCIVAVCPNMGIGKDGNFPWHPIRLSKELKHFQKMTATPSVEGKRNAVIMGRKTWFSIPEKHRPLKDRLNIVLSRELKELPHGANYLAHDLDSALAHLDSSELQDKVDLVWIIGGSTLYKEAMESPVSHRIFVTRILKDFESDTFLPEMDLNKFKLLPKFPDVPTEVQEDNGIQYKFEVYERIAHA >DHFR_calMil calMil Callorhinchus milii (elephant shark) AAVX01229006 fragment 0 MPRMLNSIVAVCPNMGIGKDGNLPWHPKRLN 2 1 KEFKHFQRMTSTPS 1 >DHFR_eptBur Eptatretus burgeri (hagfish) BJ646201 MAQNPVNVIAAVLPNMGIGWKGGLPWHSKSLVKEMKHFTRLTSAAAEGKQNAVVMGRKTWESIPEKFRPLRNRINVVLSRTLSHLPLGAHYLATDFSNALAQLSESELCCRIDHIWIIGGSSIYKEALLHPACHRIFITRIMHDFEADTFFPHIDSKRFSLCQSYPGIPEEIQEENGVQYKFEVYEVVAK >DHFR_petMar Petromyzon marinus (lamprey) Contig6033:1-27,714 GKQNVVIMGRKTWFSIPEKKRPLKNRINIVLSRELKCLPEGAHYLVPDLNSALSLIDSTPAIQEKLDGVWILGGGGVYKEAMEHSACRRLFITRVLQTMEADAFFPDIDADKFKLLP
Urochordate, cephalochordate, hemichordate and echinoderm reference sequences
>DHFR_cioInt Ciona intestinalis (tunicate) F971791 dozens of transcripts 0 MPAKDIQIHSVVACCNNGGIGFKGRLPWRLP 2 1 KEMKYFKRITTGEVEEFG GRRNaiIIGRKTWESIPKSFKPLKDRINIVISRTLDKDCEGP 0 0 DLVVNSLDELIdLLHSKPWCDKINQVFNIGGNEIYK 0 0 LIHNSQYCGKIYLTRVLADlQCDTFLENLDDNFTKLPTEG 2 1 FPEVPQGIQMDKNDLQWKVEVYEKKTT* 0 >DHFR_cioSav Ciona savignyi reftig_155 0 MPAKELKIHSIVACCNNRGIGNKGRLPWRLP 2 1 KEMKHFTSLTTGDVENLN GKRNAVVVGRKTWESIPKSFKPLKNRVNIVISRSLSKEYEGP 0 0 DAVVNSLEELVQLLQSDHWSNRINQVFNIGGSEVYK 0 0 LINNSKYCGTIYLTRVLADFECDTFLDKFDDTFKQLDTTK 2 1 FPEVPQGEQMDKNDISWRVEVYVKKS* 0 >DHFR_halRor Halocynthia roretzi (squirt) DB618016/EY457317 0 MKIHAVVACCNNRGIGKDGTLPWRLP 2 1 KEMAHFQKITKGSPPK GKKNAVVMGRKTWEEIK RPLKDRYNIVMSRSLSEKLKGM 0 0 DALVRSLGELEDLLSSGEWTGEINEVFNIGGAEICR 0 0 LINGSSLCGRLCVTQMVTDFECDAFLPEIDDGEYLR LDE 2 1 YPEDVATLLPDGEVVEANGVKWAVEVYEAI* 0 >DHFR_botSch Botryllus schlosseri (tunicate) HO068783 0 MEIHTVVVCDDNWGISKDGCLPFGTK 2 1 KEAAHFYRFIADTSHLPP GRKNVLIMGRKTWTE ETPADAPQSRSVTYNFLLSKTLKERPEEV 0 0 DGIVSSVEEFVTLMESPRWKDKIGRIYNIGGGQACE 0 0 AIYASKYCGRAYMSLVRGDFHCDTFAPNLD >DHFR_molTec Molgula tectiformis CJ399083 poor quality fragment MGRNTWNSIPSKFRPlKnRWNIVLSRTlKDPyDGM HAVVSSlDQlYKlLQDEAWKDKVNEIFCIGGAQVYE VIDNSPYIGLiFMTRIRKKYDCDTFYSEVNKDKFILLKSCP >DHFR_oikDio Oikopleura dioica (tunicate) CABV01002330/FP801526 completely different intronation from Ciona 0 MNKSGWNMILAADIKGGIGLRNDLPWRIPQDLKHFQMLTKGTPNQESVVVMGRNTWQSIPEKFRPLK 0 0 GRVNVVITNTLESENFIKCTSLEAAIAYINENHVNATKWIIGGAtLYNRGLKENFIDEVYLTRIQGDY 1 2 NCDTYVKDLYKILEVDYRLETSPKFDSKEVIGESGIKYAFERYVKNSGI* 0 >DHFR_braFlo Branchiostoma floridae (amphioxus) ABEP02022512/XM_002597864/BW702700 triplicated contig misassembly 0 MKTKKLSLVVAACNNMGIGVDGKIPWTLR 2 1 GDMKFFSRLTSGTEEA 1 2 GKQNAVVMGRKTWFSIPDRFRPLPKRLNVVLSRNLT 2 1 TPPEGAHHLAGSLEEAVKMLTETTVADTIDKVFIIGGNSVYK 0 0 DALSHPCCHRVYLTRVYKDFHCDTFFPNMDDTFKLVS 2 1 DPTIPSEMQEENGIEYKYEVYEKVA* 0 >DHFR_sacKow Saccoglossus kowalevskii (acornworm) ACQM01022605 exons 2-3 fused 0 MKKISLVAAACNNMGIGKNGDLPWRLR 2 1 KEMSFFTKVTSETKED GKQNAVVMGRKTWFSIPEKYRPLAGRYNVILSKNLK 2 1 ECPSGADMLSESLDDAIKQLSEPPLVDKIDKVYIIGGSSVYK 0 0 ESLESAACYRIYLTRVMADFDCDTFFPEFDTNLFNLVS 2 1 DPDVPSEIQEEKGIQFKYEVYEKK* 0 >DHFR_balCla Balanoglossus clavigerus (acornworm) FN986971 mQKISPVAAACNSMGIGKNGNLPWRLRKEMKYFTNVTSETVEEEKQNAVIMGRKTWFSIPEKYRPLNNRFNVILSNTLKECPAGADLLSPSLEEAVKLLSESPLEGKIDKVFIIGGSSVYKESIQSSLCHRIYLTRIMADFDCDTFFPNFDTNIFKLISDPSIPSEIQEEKGIQFKYEVYERQDV >DHFR_strPur Strongylocentrotus purpuratus (urchin) AAGJ04143548 exons 2-3 fused 0 MAEKKLNLIAAACTSkGKMGIGINGNLPWRLR 2 1 QEMAYFERLTKTAQME GMKNAVIMGRKTWDSIPEKFRPLKDRVNVVLSNSLT 2 1 ECPPGADHLCSSLNEAVKLFSSPPLSETVDMVWITGGSAVYK 0 0 DGIDSPHCHRIYLTRIMKEIECDTFFPEFDLDRFKLVT 2 1 DPAVDQDTQEEKGIQYKFEIYESSS* 0 >DHFR_parLiv Paracentrotus lividus (urchin) AM567148 MADKRLNLIAAACTSNGKMGIGINGNLPWRLRQEMAYFERLTKTSQMEGMKNAVVMGRKTWFSIPEKFRPLKDRVNVVLSSSLTECPPGADHLCSSLSEAVKLLSSPPLSQTVDMVWITGGSSVYKDGIDSPLCHRIYLTRIMKEIECDTFFPEFDLDRFKLVTDDAVSQDIQEEKGIQYKFEIYESS >DHFR_lytVar Lytechinus variegatus (urchin) JI431194 MAEKKLNLIAAACTSSGKMGIGINGNLPWRLRQEMAYFERLTKTPQLEGMKNAVIMGRKTWFSIPEKFRPLKDRINVVLSNTLTECPSGADHLCSSVSEAVKLLSCPPHSQTVDMLWIIGGSAVYKAGIESPCCHRIYLTRVLKEIECDTFFPEFDLEKFKLVVDPAVSQDIQEEKGIQYKFEIYENSS >DHFR_patPec Patiria pectinifera (starfish) DB389444/DB385866 also called Asterina MAGQKQcNLIVAACKCKDSLGIGINGTIPWKLRTDMKFFSTQTSTTAEN DKKNAVIMGRKTWLSIPDKFRPLPNRVNIVLSKTLSECPAGADHLCRSLDHAVKLLSEPPVADGIDVVWIIGGSSVYKEAMESSLCHRIYLTRIHSQFECDTFMPEIDTSKFKVVSDPAVSGERQKENDIEFNFEIHQNIQHTSTS
Protostome reference sequences
>DHFR_droMel Drosophila melanogaster (fruitfly) NM_001043255 Diptera; Muscomorpha 2-6 fused MLRFNLIVAVCENFGIGIRGDLPWRIKSELKYFSRTTKRTSDPTKQNAVVMGRKTYFGVPESKRPLPDRLNIVLSTTLQESDLPKGVLLCPNLETAMKILEEQNEVENIWIVGGSGVYEEAMASPRCHRLYITKIMQKFDCDTFFPAIPDSFREVAPDSDMPLGVQEENGIKFEYKILEKHS >DHFR_droSim Drosophila simulans (fruitfly) XM_002103136 Diptera; Muscomorpha 2-6 fused 0 MLRFNLIVAVCENFGIGIKGDLPWRIK 2 1 SELKYFSRTTKRTSDPTKHNAVVMGRKTYFGVPESKRPLPDRLNIVLSTTLQESDLPKGVLLCPNLETAMKILEEQNEVENIWIVGGSGVYEEAMASPRCHRLYITKIMQKFDCDTFFPAIPDSFREVAPDSDMPLGVQEENGIKFEYKVLEKQS >DHFR_droSec Drosophila sechellia (fruitfly) XM_002041171 Diptera; Muscomorpha 2-6 fused 0 MLRFNLIVAVCENFGIGIKGDLPWRIK 2 1 SELKYFSRTTKRTSDPTKHNAVVMGRKTYFGVPESKRPLPDRLNIVLSTTLQESDLPKGVLLCPNLETAMKILQEQNEVENIWIVGGSGVYEEAMASPRCHRLYITKIMQKFDCDTFFPAIPDSFREVAPDSDMPLGVQEENGIKFEYKVLEKQS >DHFR_droEre Drosophila erecta (fruitfly) XM_001980159 Diptera; Muscomorpha 2-6 fused 0 MIRFNLIVAVGENFGIGYKGDLPWRIK 2 1 SELKYFSRITKRTNDPSKHNAVVMGRKTYFGVPESKRPLSNRLNVVLSTTLQENELPEGVLLCSNLETALKVLENKNDVENVWIVGGSGVYKEAMASPRCHRLYITKILHKFECDTFFPAIPDSFREVAPDSDIPLGVQEENGIHFEYKVLEKQSK >DHFR_droYak Drosophila yakuba (fruitfly) XM_002097560 Diptera; Muscomorpha 2-6 fused 0 MLSFNLIVAVCENFGIGINGNLPWRIR 2 1 SELKYFSRTTKRTSDPSKHNAVVMGRKTYFGVPEGKRPLPDRLNIVLSTTLQENELPKGVLLCRNLEAAMKALEDKNEVENIWIVGGSGVYEEAMASTRCHRLYITKIHEKFECDTFFPAIPDSFREVAPDSDTPLGLQEENGIKFEYKVLEKQ >DHFR_droAna Drosophila ananassae (fruitfly) XM_001954865 Diptera; Muscomorpha 2-6 fused 0 MRRFSLIVAVSENLGIGIKGDLPWRIK 2 1 SELKYFSRTTKRTSDPSKRNAVIMGRKTYFGVPESKRPLPNRLNIVLSTTLQPEDLPKDVMLCPNLEAAMKHLEGDDQIENVWVVGGSGVYAEAMDSNRCHRLYITRIHQKFECDTFFPTIPESFREVAPDPDTPAGVQEENGIEYEYKVMEKETK >DHFR_droPse Drosophila pseudoobscura (fruitfly) XM_001359349 Diptera; Muscomorpha 2-6 fused 0 MLRFNLIVAVSENFGIGLKGDLPWRLK 2 1 SELKYFSRTTKRTSDPNKRNVVIMGRKTYFGVPASKRPLPERLNIVLSTTLKPAELPSDVVLCHSLEEAMKHLEEETLRDQIENVWIVGGSGVYEEAMASSRCNRLYITKIQQNFECDTFFPSIPDSFREVPPDADTPVGVQEENGIKYEYKVLEKRE >DHFR_droPer Drosophila persimilis (fruitfly) XM_002017170 Diptera; Muscomorpha 2-6 fused MLRFNLIVAVSENFGIGLKGDLPWRLK 2 1 SELKYFSRTTKRTSDPNKRNVVIMGRKTYFGVPASKRPLPERLNIVLSTTLQPAELPSDVVLCPSLEEAMKHLEEETLRDQIENVWIVGGSGVYEEAMASSRCNRLYITKIQQNFECDTFFPSIPDCFREVPPDADTPVGVQEENGIKYEYKVLEKRE >DHFR_droWil Drosophila willistoni (fruitfly) XM_002070058 Diptera; Muscomorpha 2-6 fused 0 MLRFNLIVAVCENSGIGLKGDLPWRIR 2 1 SELKYFSNTTKRRLDSSKRNVVIMGRKTYFGVPPSKRPLPDRVNVILSSTLKSNELPNEVLIYSSLEGAMQELEKQKDQIENVWIVGGSSVYAESMASPRCHRLYLTKIYAEFECDTFFPKIPESFREVSPDKDTPPGIQEENGLKYEYKVFEKQE >DHFR_droMoj Drosophila mojavensis (fruitfly) XM_001998634 Diptera; Muscomorpha 2-6 fused 0 MLKYNLIVAVCENFGIGFKGDLPWRLK 2 1 SELKYFSRRTKRINDPSKRNVVIMGRKTYFGVPPSKRPLPDRLNIVLSTTLTQKELPEGVLLCGSLDEAMQTLEKKPLCDQVENIWIVGGSGVYAEAMESPRCHRLYITKIQERFECDTFFPHIPASFHEVELTEDTPHGVQEENGIKFEYKILEKREHII >DHFR_droVir Drosophila virilis (fruitfly) XM_002056347 Diptera; Muscomorpha 2-6 fused 0 MLRYNLIVAVCENFGIGLKGDLPWRLK 2 1 SELKYFSRTTKRTRDPAKRNVVIMGRKTYFGVPPSKRPLPDRLNIVLSTTMSRAELPNDVLLCRSLEEAMKMLESESEPWCDQIENIWIVGGSGVYEEAMASPRCYRLYITKIQEQFECDTFFPNIPADFHEVPLDEDTPHGVQEENGIKYEYKILEKQ >DHFR_droGri Drosophila grimshawi (fruitfly) XM_001990307 Diptera; Muscomorpha 2-6 fused 0 MLKFNLIVAVCENFGIGFKGNLPWNLK 2 1 SELKYFSRTTKRVCDPSKRNVVIMGRNTYMGVPPSKRPLPDRLNIVLSTTMSAADLPKEVLLCRSLNEAMETIESPEWCKQVENVWIVGGSRVYAEAMDSERFHRLYLTKIHEKFECDTFFPEIPDCFKEIPLDEHTPDGIQEEDGIKYEYKILEKQ >DHFR_gloMor Glossina morsitans (tsetse_fly) EZ423753 Diptera; Muscomorpha MLKFNLIVAVSKNFGIGLKGGLPWELKSELRYFSELTKRVFDSTKRNVVIMGRKTYFGIPLNNRPLRNRLNIVLSTTLNKVGELPEEVLLQPNLEAAMKFLEDNNTLKSNIENIWIIGGASVFKEAMASKRCHRLYITEIQSSFESDVFLPTIPNDFEQIIPGPEVPQTVQVENCICFRYKVLEKRE >DHFR_haeIrr Haematobia irritans (buffalo fly) AY099474 Diptera; Muscomorpha MLKFSLIVAVCENFGIGIKGDLPWLLKSELKYFSTTTKRVHDPSKRNVVIMGRKTYFGVPESKRPLQQRLNIVLSTTLKQSDLPKDVLLYPNLESAMSFLENEKDINQQIETVWIVGGSGVYKEAMASPRCHRLYLTKIYAKFDCDTFFPDIPADFKEVDLDPETPKGIQEENNITYEYKILEKQDHK >DHFR_sarCra Sarcophaga crassipalpis (flesh_fly) EZ609788 Diptera; Muscomorpha MLKFSLIVAVCENFGIGIKGDLPWKIKSELKYFSSTTKRVRDPSKRNVAIMGRKTYFGIPESKRPLPERLNIVLSTTLKPSDLPSDVLLYPNLESAMKMLEENEKLKQEIETVWIVGGSGVYAEAMASQRCHRLYLTKIQASFECDTFFPKIPDDFVEVELDEETPKGIQEENNIKYEYKILEKKT >DHFR_culQui Culex quinquefasciatus (mosquito) XM_001864570 Diptera; Nematocera exons 2-6 fused 0 MKKFSLIVAVCSNGGIGIKGDLPWRLR 2 1 SELRHFARMTKRVADPGKRNAIIMGRKTYFGIPEGRRPLPDRLNVVLTRDPASNTYPEGVLVCTSLEDALAKIQDLVENIWIIGGSAVYKEAMESRHCHRIYLTEILAPFECDAFFPEIGKEFRLVGNDADLAEEVQEENGVRFQYKIYEKKDID >DHFR_anoGam Anopheles gambiae (mosquito) XM_319471 Diptera; Nematocera exons 2-6 fused 0 MSKKFSCIVAVCENRGIGINGDLPWKLK 2 1 QELKYFSHTTKKVNDADKRNAVIMGRKTYFGVPESKRPLPERLNIVLTRNAAAYTFPPDVLVCGSLQEALHKLDSTDHGEHIENIWVVGGNSVYKEAMESDRCHRIYLTEIKKQFDCDAFFPEIPNSFMVIDNDPDTPSGVQEENGIQYVYKIYENKQQ >DHFR_aedAlb Aedes albopictus (mosquito) X60192 Diptera; Nematocera exons 2-6 fused 0 MKKFSLIVAVCANGGIGIKGDLPWRLR 2 1 QELKYFSRMTKKIQDSGKRNAIIMGRKTYFGVPESKRPLPERLNIILTRDPSANAYPSEVMVCTSMQEALKKLDEAPLVNEIENVWIVGGNAVYKEAMQSDRCHRIYLTEIKETFECDAFFPEITSDFQLVKNDDDVPEDIQEENGIQYQYRIYEKVPK >DHFR_aedAeg Aedes aegypti (mosquito) AY432005 Diptera; Nematocera exons 2-6 fused MKKFSLIVAVCANGGIGIKGDLPWRLR 2 1 QELKYFSRMTKKITDTSKRNAIIMGRKTYFGVPESKGPLPERLNIILTRDPSANTYPSDVMVCKSMQEALTKLDESPLANEIEKIWIVGGNAVYKEAMQSERCHRIYLTEIKETYECDAFFPEITSEFQLVKNDDDVPEEIQEENGIQYQYRIYEKLPK >DHFR_armSub Armigeres subalbatus (mosquito) EU205282 Diptera; Nematocera MKKFSLIVAVCANGGIGIKGDLPWRLKQELKYFSRMTKKKQDTSKRNAIIMGRKTYFGVPESKRPLPERLNIILTRDPSAHVYPPDVMVCTSMQDALNRLDETPLVDEIENIWIVGGNAVYKEAMQSARCHRIYLTEIKKTFECDAFFPEINSDFQLVKND >DHFR_danPle Danaus plexippus (monarch) AGBW01009470/AGBW01006850 Lepidoptera 0 MSKVKLNLIAAACENMGIGSNGSLPWRLK 2 1 KEMEYFTTMTTKVKDA SKINAVIMGRRTWDCIPNKNRPLENRLNIVLTRHVDVIKDK 0 0 VPKDVVVVSGLDEAVSYIIDRNDVESTWVIGGSSVYQ 0 0 ASMDHPNCGKIYLTEIQKTFDCDTFFPNINRQRFHLVDEDDIPRDIQTEGDISYYFRVYKRL* 0 >DHFR_bomMor Bombyx mori (silkworm) BABH01041437+BABH01041438 Endopterygota; Lepidoptera 0 MSRTQLNLIAAACENMGIGINGTLPWKLK 2 1 KEMAYFTTMTTSVSDK KKVNAVIMGRRTWDCIPIKYRPLSNRINIVLTHNVDYVKKQ 0 0 VPEGVIVVPGLDEAIDHIDSRSDIESTWVIGGSSIYK 0 0 AAMEHPNCGKIYLTEIQKSFDCDTFFPDINKQRFHLVNEEEVPGEKQSEGDINYYYRIYKRL* 0 >DHFR_helVir Heliothis virescens (budworm) AF104106 Lepidoptera MSQVKLNLIAAACDNMGIGVNGALPWRLKKEMAYFTTMTSKVSEPTKVNAVIMGRRTWDCIPDKYRPLQDRVNIVLTHNVDSVKENVPEGVMVFPGLDEAIKYIEGREDIESTWVIGGSSIYRAAMTHPNCGKIYLTEIQKSFDCDTFFPNIDKQQFHLVDEEQIPGEKQVEGNISYYFRVYKKL >DHFR_triCas Tribolium castaneum (beetle) AAJJ01000197 Coleoptera exons 2-4 fused 0 MVIKFDLIAAACENMGIGKNNDLPWRLK 2 1 SELAFFSQMTTQTSDESKKNVVLMGRKTWDSIPPKFKPLHQRFNFIMSRAGVNLEGYKDCFSFKSLDEVISKLQDEKFQQLYENVWVIGGSYIYE 0 0 ATMASKYFHRLYLTKVLKTFDCDTFFPKIRDDLIEVRYL 2 1 DPRVPEGVQEENGIQFVYHVYQNPDFNKL* 0 >DHFR_denPon Dendroctonus ponderosae (beetle) GO487443 Coleoptera MALKLNLIVAASENWGIGKNGTLPWKLKKEMAFFRTMTSATEDKSKKNVVIMGRRTWESIPAQFKPLPNRINFVLSRSGLNLDQYSNTQSFTNFREALDKLEESAFRQLYENVWIIGGSSLYNETFRSPHFHKLYLTKIHKEFDCDTFLP ALPGNLKEINDPAVTED IQEENDIEYTFQVFEVIN >DHFR_apiMel Apis mellifera (bee) HP575683 Hymenoptera 0 MNLHLIAATCEGMGIGIKGTLPWKLK 2 1 SELAFFTYMTTNTKNPNKRNVVLMGRRTWESIPKENRPLKNRINIVLTSQSL 2 1 DYGNDVIVCKNIPHAFEVIEKIKDRIENIWVTGGSSVYK 0 0 EAMESPNFYRLYLTRIKKYFECDTFFPTIPNDFVLTE 2 1 DPNIPQGIQEENDIQFVYEVYKKNRFLIWFQNNIFRIL* 0 >DHFR_bomImp Bombus impatiens (bumblebee) JI114531 Hymenoptera 0 MNLNLIAAACEGMGIGVKGTLPWRLK 2 1 SEMAFFTSMTTNTKDPNKKNVVLMGRRTWECIPKKYKPLKNRINIVLTSQSL 2 1 DYGDDAIVCKSIPHALEVIKKPTLKDQIEGIWVIGGSSVYK 0 0 AAMESPNFHRLYLTRIKKHFVCDTFFPTIPNNFVLID 2 1 DPNIPQGVQEENDIQFVYEVYEKNRFLYLKII >DHFR_eugCor Euglossa cordata (bee) HP973249 Hymenoptera distal frameshift fixed MSLNLNIIAAVCEGMGIGVKGSLPWKLKSEMAFFTSMTTQTKDPK KNVVIMGRRTWECIPTKYRPLKDRINVVLTSQNLDYGVNAIICKSMSHALELLKKPPFSDEIESVWVIGGNLVYKAAMESPNFHRLYLTRIKSILTVIHFFPPIPNDFALIQDPNIP >DHFR_nasVit Nasonia vitripennis (wasp) XM_001605731 Hymenoptera 0 MQVKLKLIAAACENMGIGVNGDLPWRLRK 2 1 EMDFFTKMTSTTKDENKKNVVLMGRRTWESIPKKFKPLSNRINMVLTSQPL 2 1 DLGEDAIVCKNLPEAIEKISQSPLKDKVEQVWVIGGSLVYK 0 0 TSMESPNFYRLYLTRVKKDFKCDAFFPEIPNDYILVK 2 1 DPEVPEGIQEEKDIKFEYEVYEKKQN >DHFR_copFlo Copidosoma floridanum (wasp) JI834972 Hymenoptera MQVKLKLIAAACDNMGIGINGDLPWRLRKEMDFFTKMTSTTKDPNKQNVVLMGRKTWESIPKKYKPLANRINVVLSSQPLDLGKDAIACNSLPEAIQQISQDHLRDKVEQVWVIGGSSIYQAAMDSPNFYRLYLTRVKKHFDCDTFFPEIPKDYALVKDPEVPEGIQEEKGINFEYEVYQKA >DHFR_attCep Atta cephalotes (leafcutter-ant) ADTU01012310 Hymenoptera 0 MPPKLELIAAACENMGIGVNGNLPWRLK 2 1 AEMAYFTRMTTNTRNKNKRNVVLMGRRTWECIPEKYRPLKDRINMVLTSQSL 2 1 NYGDDAIACKSILHALDVISEMQNEVERVWVIGGSSVYK 0 0 SAMESPHFGRLYLTRIKKKFECDTFFPSIPNDLVLIE 2 1 DSTVPQGIQEEKGIEFVYEVYERR* 0 >DHFR_camFlo Camponotus floridanus (carpenter_ant) XP_393902 Hymenoptera MPPKLELIAAACENMGIGINGGLPWRLK TEMAFFTRMTTDTKDKDKKNIVLMGRRTWDCIPEKYKPLENRINMVLTSQSL NYGDKAIICKSIPHAIEVIAEMQNQVERVWVIGGSRVYK DVMGSPYFGRLYLTRIKKEFKCDTFFPPIPSGFVLIK DPTVPQHIQEEKGIKFIYEVYEKK >DHFR_harSal Harpegnathos saltator (jumping_ant) XP_393902 Hymenoptera MLPKLELIAAACENMGIGIKGDLPWRLK TEMEYFTRMTTETKDKNKKNVVLMGRRTWDCLPETYRPLRNRINMVLTSQL MNYGDKAIVCTSIPNALEIITKIQNEVERVWVIGGSKVYK EAMESPYFGRLYLTRIRKRFECDTFFPPIPNNFVLVQ DPIVPEDIQEEKGIQFVYEVYEKR >DHFR_linHum Linepithema humile (argentine_ant) LH21356-RA Hymenoptera MQLKLELIAAACENMGIGNNGALPWRLK TEMEYFTRMTIDTKDKNKKNVVLMGRRTWDSIPIKYRPLNDRINMVLTSQSL DYGDKAIVCKSIPHALEVIAGMQNQIERVWIIGGSSVYK DAMESPHFGRLYLTRIKKEFECDTFFPPIPNDFVLVT DPAVPQDVQEEKGIKFVYEVYEKR >DHFR_pogBar Pogonomyrmex barbatus (harvester_ant) B10193-RA Hymenoptera MSHKLELIAAACENMGIGINGDLPWRLK TEMAFFTRMTTETNHTNKKNVVLMGRRTWECIPDKYRPLKDRINMVLTSKSL DYGNETITCKSISHAFDIITQMQNQIERVWVIGGSSVYK SAMESPYFGRLYLTRIKKKFECDTFFPPIPNDFVLIE DPTIPQGIQKENGIEFVYEVYERR >DHFR_solInv Solenopsis invicta (fire_ant) AEAQ01011575+AEAQ01011576 Hymenoptera MPPKLELIAAACENMGIGVNGDLPWRLK TEMAFFTRMTTDTKQNNKKNVVLMGRRTWECIPKKYRPLKDRINMVLSSQSL DYKDEAIVCKSIPHALDVISGMQNQVERVWVIGGSSVYK SAIESPYFGRLYLTRIKKKYECDTFFPLIPNDFVLVE DPTVPQGIQEENGIEFVYEVYERR >DHFR_bemTab Bemisia tabaci (whitefly) EZ942456 Hemiptera MSSLRLSIIVAMTADNGIGFKNSLPWSLPNELRNFAKLTKNCKDKSKQNAVVMGRNTWESIPAQHRPLKDRLNVVLSSTLKDAGEGVLVCPRLKDALTLLEENYQNKIENVWIIGGARVYEDALNLSNCHKLHVTHIQEKPECDVFFPTIPRDFQIITTSEMVEENGFHYNYQVYERRTSPDGEANGIEDSTKS >DHFR_acyPis Acyrthosiphon pisum (aphid) NM_001162022 Hemiptera one exon MVYSVIAAVSKNGGIGYKGNLPWKIKKEMEYFNLMTTRVNLKGVQNAVIMGRCTWQSIPDKYRPLKGRINVVISKTLNSVPEGVLLYPKLEEALKSLYLNDRIENLWVIGGSGLYKEAVNDKNCKKLFITKIDQEYLCDTFFPDFDTKKFEETSEANVPKGIQEDNGIKYEFKVFKRL >DHFR_blaGer Blattella germanica (cockroach) FG125641 Orthopteroidea MNLKLXLIVAVSENMGIGLNGDLPWRLRKELAHFSRLTKRTIDSNKQNAVIMGRRTWESIPIKNRPLPERLNVVLTKQELDFGKEVLVCNSLNSALEKLCEPRLDKTIESIWVIGGSSVYKEAMSSPHCHRIYLTKIFKTFECDTFLPEIPGNSFKIVRDPDVADEIQEENGLTYEYQVYERILV >DHFR_pedHum Pediculus humanus (louse) XM_002432121 Phthiraptera 0 MVNNFNLIAAVCSNNGIGYKGNLPWNLR 2 1 KELQYFNRMTKDVKNPEKKNAVIMGRKTWDSLPHNWKPLPGRYNFVLTTQSL 2 1 NLDGAVVCSDLNDLIKKINSPDYSNLIETAWVIGGSKVYESVLKYGLCHRFYLTRIKKEFECDCFFNYDFTKDFKEVS 2 1 DDRVPKGVQKENEIEYVFHVYEKVNESKDSSN* 0 >DHFR_onyArc Onychiurus arcticus (springtail) EW753645 Hexapoda; Collembola MGPKLYIIAAACENMGIGYKNDLPWKLRNEMAYFNRMTQIVENPGSGSELKNAVIMGRRTWDSIPEKFRPLRNRLNIVLTSRPDFKQPNVLSYPGWYEAVKDLATPEKSLQIDKVWVIGGNSTYKLAVESPDCHRIYLTRVLKHFECDAFFPEFDKSKFRLANDPNVPQDIQEEDGIQYHFEI >DHFR_calCle Caligus clemensi (sea_louse) BT080999 Crustacea; Maxillopoda; Copepoda MTSLKCVKINVIAAACKSSRGIGKDNDLPWKLPTDMKYFREKTASTTEIRVGVIMGRRTWESVPPKFRPFKNRFNVVLTSREDFEAKGAVIARTLEDAVKKCSNNVDSIWVIGGSGVYAEALNKYPCRVYLTEIDKEFDCDAFFPPLDEKKYKLASVSPDHQENDITFRFKVYDVIEL >DHFR_lepSal Lepeophtheirus salmonis (sea_louse) BT121746 Crustacea Copepoda 0 MSKSIPTLHVIAAACRPTNGIGKNNTLPWNLPTDLKYFKTTTSSTPDSKVAVIMGRRTWESIPSKPRPLKNRINVVLTTNSNFVAEGAILA SSLEEGIKSCMDQADKIWIIGGANLYKEAMQYYKCQIFLTEIDKEFECDTFFPSIDSTKYKEVSVSEDHVENGITFRFKRFERLLQENN >DHFR_litVan Litopenaeus vannamei (shrimp) FE141783 Crustacea Malacostraca MSVRLNIIVAACENHGIGINGELPWKLREEMKYFSRMTKATKSLEKQNAVMMGRKTWESIPAKFRPLPGRLNLVISSRAKDPGPEFNGSTVCASFKEAIDEVQSRTDEVESLWAIGGSSIYE MALESEHLHRIYLTRILKDFECDTFLPLFDPKKFQIVTD PAVPAELQQEGDITYKYEIYEKVK >DHFR_celPug Celuca pugilator (crab) JO488787 Arthropoda; Crustacea MGPRLNIIVAVAENHGIGKGGELPWRLREEMKHFSRMTKRLDSPATQNVVLMGRKTWESIPLKFRPLPGRLNVVLSSQMKQQQDSSGKVIVCQDFESALKSVSTENPEVETVWVIGGHSVYKMALESEHLHRIYLTKVMKEFECDVFFPSFKMNEFKLVEDPDVPGQV >DHFR_dapPul Daphnia pulex (water_flea) FE400591 Crustacea Branchiopoda 0 MKLNLIVATASNMGIGFQGTIPWRLK 2 1 KDMALFAKLTKNTNDTNKQNAVVMGRKTWESIPEKNRPLSNRLNIVLSSSQQ 2 1 IENTLTCKSLESAMQLLQTPPYLDQIENIWIIGGASVYK 0 0 EAMEHPSCHRIYVTHILKDFECDVFMPAIDPAKFSLVG 2 1 DSKVPEGTEEENGISFNVKVYEKV* 0 >DHFR_ixoSca Ixodes scapularis (tick) EW930272 Chelicerata Arachnida 3 re-intronated exons 0 MCPGVQESLQYFAIAAMCHNRGIGVLNTLPWRLKKEMAYFSRITSQAAEGKTNAVVMGRNTWDSIPPKYKPLPGR 2 1 VNVVVSRTLEKVPDGHHVARSFSDALSLLQPLVDSGK 0 0 VDKTFVVGGAQLYREALADPRCTRVYLTEIDADFECDVFFPELELGQFRLVQEEGVPQEEQTEGGLTYRFNVYQRISSGSV* 0 >DHFR_ambMac Amblyomma maculatum (tick) JO841362 Chelicerata Arachnida MCPSHKSVVSCFAIAAMCRNRGIGFKNALPWRLKKEMAFFKRMTSEAAEGKQNAVVMGRNTWESIPPKFRPLNNRINVVVSKTLTEVPEGHHVASSFPAALQLLQTLVDTGKVDKVFLVGGAQLYREALESGHCTRIYLTEVDKDFECDVFFPEFDNSVFSPVEEEGVPQEPQQEDGVTFRFRVYERVQN >DHFR_varDes Varroa destructor (mite) ADDG01075186 Chelicerata; Arachnida one exon MAVPPLCAIVAVCKASKGIGNKGSLPWGRSLPNEMKHFARVTTNTTDPDKCNAVVMGRRTWESIPENRRPLPRRINIVISSGLCQESVPEGVKVAKSFDEALEIAGTGCCKGQSRVERIFVIGGTQVYVDAMRHPAMDTVYLTEVLGQFECDTFLNIDTRKFTDIEDPAVTNEEQEENGIRYKYRVLKKIPQ >DHFR_perSed Peripatopsis sedgwicki (velvetworm) FN242195 Onychophora MHRYLNLVVAVCNNLGIGIGGELPWRLRGDMKFFSKLTSETKDSEKTNVVIMGRKTWASIPEKFRPLPKRTNIVISSSLNSVPEGVLIARDFEHAMSLAFGLQQDNIVEQVFVIGGSSVYEQALKSDFPCRIYLTRVMGDFKCDTFFPKLNVDKYALVKVPEIPEEVQEENGISYLFQVLQEKVES >DHFR_milTar Milnesium tardigradum (tardigrade) EZ761524 --------VAACRNGGIGIRGDLPWTLRGDMRFFTKITSQTKDPQKKNAVIMGRKTWFSIPERFRPLSKRINVVISRTMFGIQPNAIQTQSLEEALDLLSKEPLSSEIETVWIIGGESLYKMAIHSPQLYRVYFTNVMADFECDTFFPTQELQRMKVVSDADVNGDVQMENDVKFQHEIYEP >DHFR_triSpi Trichinella spiralis (nematode) ES273161 Nematoda Enoplea MAQVNIIVAICEKYGIGKKNSLPWHLSKEMQHFKKMTTSVSDPNKINAVIMGRNTWYSIPEKYRPLSGRFNIIISGTMPQLSDEDVIVTWHWREAIEIVEHPISKKAIETFWICGGAKLYNDVIESGLWNRLYVTWIMKEFDCDAFFSFPDKNTIKLVEDDRIPSDIQVEKGIHYKYEVYENCMKMQS >DHFR_xipInd Xiphinema index (nematode) CV509322 Nematoda Enoplea MNLIVAACDNMGIGHGNELPWPKLPNESKHFLKLTAGTRDPGKQNAVIMGRKTWEIIPVEHRPLKHRLNVVISRTLSRKDVPPGVQLFEDFEPAMRYLLSPQVSQNIETIWNVGGSEIYALGLDHPSFHRLYFTRVSGTYDCDTYFPQVDWTMLKRVEDPNISTGLHEENGVQYKFETYEHAF >DHFR_caeEle Caenorhabditis elegans (nematode) NM_059963 Nematoda Chromadorea 4 exons re-intronated 0 MRKMNLIVAMDAEGGIGKNGVLPWRIKKDMQYFASVTKNVSDQSK 0 0 RNAVLMGRKCWESIPVTRRPLAGRLNIVLSRQLPAQKSDDYIVV 2 1 NSLEAAMKLLSEPPFVDSIETIWNIGGAEIYDLALRENLVDEIHLTRIFKN 2 1 FEADVHLKSLDFSKMEKVQNAEVSSENSEIFEENGLKFEFCKWKVVENH* 0 >DHFR_melInc Meloidogyne incognita (nematode) CF803120 Nematoda Chromadorea 3 exons re-intronated MNIIAAVDENFGIGKNNSLPWRLPKEYKHFINLTTTTKNPNKINAVLMGRKCWESIPEKYRPLKNRLNIVMTKTWVTPEF 0 0 VGENLIFINSLDSLNLILESKPYENLIETIW 2 1 NIGGKQIYSLGIEHQNLNKIVLTKIDKNFDCDVKFPEINWNEFIEEENGEIVEEKGLCWKAITYIKK* 0 >DHFR_ascSuu Ascaris suum (roundworm) JI215038/AEUI01062849/AEUI01051380 Nematoda Chromadorea 0 MVSPKLPINIIVAMDSRGGIGKNGALPWHIPEDLKYFQTMTTKTIDPTKQNAIVMGRKVWESLPAKWRPLKNRLNVVLSNS 0 0 MDDPHDGSYIVTRTFESAIDILNGMSDKIETIWDIGGRRPYEEGLKSSQLRQ 0 0 LYVTFVEGDFDADVFFPDVDFKKFSKQNGDKQSSEHHYEGITYRFETFTALHTDTELS* 0 >DHFR_schMed Schmidtea mediterranea (planaria) DN307116 Platyhelminthes Turbellaria 6 standard exons 0 MKRLNLIVAACENQGIGKNGKLPWNLK 2 1 NEMIYFNNITTSVSDS 1 2 NKQNVVIMGRITWESIPNKFRPMPKRINIVISSTLSR 2 1 DHDNLTGTYLFNNLSEALSFTNDELSDKVEEVFVIGGSSLYK 0 0 AALSSSYPLRLYYTKIFESLDCDTFFPAFEWEQFKSVK 2 1 LDHVPSEVQQENGIRYQFMVLDRC* 0 >DHFR_schMan Schistosoma mansoni (tapeworm) XM_002580511 Platyhelminthes Digenea 0 MRLNVVVAVSENWGIGKGGGLPWKIK 2 1 KDMEFFKTVTTKAHP 1 2 GLKNAVVMGRVTWESIPESFKPLKDRINIVVSSTLSH 2 1 APSFVQVVPSLNAAIDLLYNEEFSSIVDEVFIIGGYRLYK 0 0 EALKQS YPVRIYCTHILSEVDCDTYFPKVDWDKLKK 2 1 VDLPDIPADTFTENGFTFKFCVYDVPSEQFI >DHFR_taeSol Taenia solium (tapeworm) PEL761437 Platyhelminthes Cestoda MGLRRLNVIAAVAKNGGIGKENKLPWHIREDMAFFSRITSTAQEGKKNAVVLGRRTWLSFPPKFRPLPNRVNVLVSTQLESVPEGAYLVKSFEDSLRLVESLVDGGEVDEVFIIGGHGLYKEALEQEVYPVRLYYTHIMKDFDCDTFFPSVDWERFTPIQLDTVDSNPKYCGEIEFRFAVYEKGPHRFHDN >DHFR_aplCal Aplysia californica (sea_hare) AASC02008512 Mollusca Gastropoda one exon MTSTKLNIVVAVCTNMGIGIEGRLPWRLKQDMAFFKQLTVETQDEQKKNMVIMGKKTWMSIPTKFRPLQDRVNVVLSTQLTEPPSGALLASSLKEAVNLAKSDANVENVFVIGGASVYREAVEGDCPCRIYLTRVDKEFECDTFFPKFDTDVFERIQNPNNVPTGINVEGDLSFTFEVYEKNLFPH >DHFR_lotGig Lottia gigantea (limpet) FC707856 Mollusca Gastropoda one exon MCTKRLNIVVAACKNNGIGVNGSIPWRLKKDMAMFRHITSDTVDESKQNAVIMGRKTWMSIPDKFRPLKNRVNIILSNTLSESPDGTYIVKNFQQALSLVNSDKMKQQIESVHIIGGSSVYKEAMESDYDCRVYLTKVDADFECDTFLPPIEESLFQRVKNPSNIPSDVQEENGIQFTYEIYDKTTDQSDRESPVSR >DHFR_phyAcu Physella acuta (snail) BW985974 Mollusca Gastropoda MPKLNIIVAACNNNGIGIEGRLPWRLKSDMSFFKQITLRTQDAEKKNAVIMGKNTWFSIPSKFRPLVGRINVVLSTQMTDAPEGTQLAKSLNQAVSLLESLNVENIFVIGGSTVYKQAMEGEHPCRIFLTRIAADFLRDTFLPDIPN >DHFR_pinMax Pinctada maxima (oyster) GT283724 Mollusca Bivalvia MSKCKLNLVVAACNNRGIGIDGQLPWRLRKDMDFFKKITTETNDPEKRNAVIMGRKTWFSIPEKFRPLSKRINIILSREMKEVPENTYVARSLDEAVQLVSDGGELADRVESIHIIGGSSVYKEALASSHPCRIYFTRVLADFKCDTFLPEFDENKYQKLENMENIPTEIMTEKDINF >DHFR_mytCal Mytilus californianus (mussel) ES395435 Mollusca Bivalvia MSKTKSTLNLVVAACNSRGIGINGKLPWRIRKDMDFFKKITMETKNPDKKNVVIMGRKTWFSIPEKFRPLPKRINIILSREMKEAPPGVYIARSFDDAITMVNKDLSDKAESIYVIGGSSIYKAAMDSDYDIRIFLTKILADYDCDTFLPQFDENLYKIIENYEGVPKGKQTENDIDFIFEVYEKVR >DHFR_dreRos Dreissena rostriformis (quagga_mussel) EY433849 Mollusca Bivalvia MSMPRLNLVVAICNNNGIGINGSLPWKLRKDMDFFKTITMTTTDPEKQNAVIMGRKTWQSIPENFRPLRNRINIILSRDMNAPNGTHVARSLPDALSLLSSEELADRVEQIHIIGGSSVYKEAMTGPHPCRLYLTRILADFECDAFLPEIDDSSFTKIRNPDNVPTDLQEENGLKFRFEVYDKL >DHFR_alvPom Alvinella pompejana (bristleworm) GO125274 Annelida Polychaeta MPPTLNLIVAMCNNNGIGIQGKLPWKIK GDMAFFRKMTTETKNPAKMNAVIMGRKTWFSIPEKNRPLNKRMNIILSQTMSSVPDGVHLARSFSEALNLISTKPLADIIEGIFVIGGASVYKEALESSYPCRIYLTRVFADVPCDTFMPTFSGFKQIECAEIDNTVQKENDWQYKFEVYEKV >DHFR_helRob Helobdella robusta (leech) EY386022 Annelida Clitellata six exons 0 MQPKLQIVVALCVKNRGIGLNNSIPWKLP 2 1 GDMTFFRKLTSETSIL 1 2 GSKNAILMGRKTWDSIPSNLKPLKNRLNVVISRTLE 2 1 cPDInGRLHVSKSFEDAVEFVASDKSIDKIFIIGGSSIYD 0 0 LALASSTCYNVFITEISNDFQCDTFFPKFNQDAYKLIK 2 1 YPGHSTSVQFENGIGYQFTCHHKVSK* 0
Cnidarians, ctenophores, trichoplax, sponge and choanoflagellate reference sequences
>DHFR_nemVec Nematostella vectensis (anemone) ABAV01013911 Cnidaria six exons MAAVGVRFSCIVAMDLKRGIGKNNDLPWKIK GDMKFFTEKTSEVKTE GKQNAVIMGRKVWESIPEKFRPLKGRLNIVLSRTLS EPPQGAQLCRSFEQALTILSTDPYTKKIENVFVCGGSALYK DAMAHSACTRIYITYIDQEFDCDVFFPEFDQNTYHLVE DPDVPSVQHEEKGIKYKFCVYDRCQ >DHFR_acrMil Acropora millepora (coral) EZ020360 GO002175 Cnidaria Anthozoa Hexacorallia VAVADNGGIGKENRLPW HLK GDMKFFSHLTSTVATE GKQNAVIMGRNTWESIPKKYRPLPRRLNIVLSRKMS EPPEGAVLCSSLQDALDRLRSAPYTDTVEKVFVIGGATVYE EAMQHHQCYRLYITHIYKDFECDVKFPEFDKRIYKETR DIDVSSEMLEEKGIPYEFKVYQKD >DHFR_hydMag Hydra magnipapillata (hydra) XM_002157178 Cnidaria Hydrozoa one exon MVQLRKVHCIAAIDSEWGIGLKGKLPWNLPREYKFFQNITTKVISEGKQNAVIMGKNTWFSIPQQHRPLKNRLNVILSSSFIKEDYPKNVLLESSLEAAILRLSDEFYANTVENIFVIGGSRVYKEAMEKFCDKIYLTKIEQDYSCDVFYPIFDTNKFKEIDDEEVDKNKQIENGVSYTFHVYSSNYTV >DHFR_mneLei Mnemiopsis leidyi (sea_walnut) AGCP01012173 Ctenophora exons 2-3 fused 0 MKLRFNIIVATDLEGGIGKNNNLPWRCR 2 1 KEFRHFTDTTSKLQNGKQGAAVMGRKTWESIPEKFRPLKNRHNFVISSTLS 2 1 VANPGNVPVYKTISDCMTALDQDKD IGSVWIVGGWGIYK 0 0 EAMESERLHRMYITTVQGKFDCEVFFPSFDERNFNLVI 2 1 DPDIPNETVTEDNGISWSVSVYERK >DHFR_triAdh Trichoplax adhaerens (placozoa) ABGP01000098 same six exons 0 MQKNFRMIAAMTRDRGIGYKNDLPWKLR 2 1 NEYKYFARLTTSTKDS 1 2 AKKNAVLMGRNTWVSIPEKNRPLRNRINIVISSQLR 2 1 AEEVPEGVHVVASIPEVIELVQSPLLKDIVETVWIVGGAPVYK 0 0 GFMSHPQCDRIYLTKLDANYECDVFFPEMSDDFKEIS 2 1 DPDVSQEVQEENGLKYTFHVYQRN* 0 >DHFR_subDom Suberites domuncula (sponge) GH558547 Porifera Demospongiae MYIMVAKEIKSGIGNKGKIPWPRINTDMNFLETMTKKTRNKTKQNAAIMGRLTWESIPAKYRPLPERFNIVLSSKKFEDIAPGAHMVCSSLSFAMEKLRAPPLSDQIENVWIFGGAGVYEESMKHPDCDKLYVTEVLQEFPCDAFCPPIDSDIYKLIDHSDDPDVPKGEFVENGVKFKFEVYQRQ >DHFR_monBre Monosiga brevicollis (choanoflagellate) ABFJ01001321 0 MRVALVVAATKAGGIGHQGQLPWHLK 2 1 ADMQHFRDITTKTEQP 1 2 AAINAVIMGRKTFESIPDKFRPLHGRLNVVLSTQKP 2 1 VSLPLHTPHDTNPPQPLPHPACLAHPQPEVIETAYIIGGASVYN 0 0 EALAKPEYSHYIFMTKVHKDVLCDTVMDMKHLDAYEHDPNFPVq 2 1 * 0
Fungal, microsporidial and amoebozoan reference sequences
Fungi are of special interest because of an existing crystallographic structures in Candida albicans, C. glabrata and Pneumocystis carinii (1AI9, 3CSE, 1CD2). A small sampler of the available sequences is provided below. Microsporidia, obligate intracellular parasites, are today considered a subgroup of 'zygomycete' fungi (for which only DHFR of Rhizopus is available). DHFR is evolving very rapidly within this group -- for example, between two species within the genus Encephalitozoon the percent identity can be as little as 61%.
Encephalitozoon are also odd in having immediately tandem copies of DHFR and TS (thymidylate synthase). These cannot be fused genes because reading frames are out of register (ruling out a linker domain), nor does the intervening DNA contain an intron (which are exceedingly rare in this genus and exhaustively listed). However, a fused DHFR-TS could readily arise from a simple deletion; no such construct has been tested for functionality to date. The basal fungus Rhizopus does not have tandem or fused DHFR-TS.
>DHFR_canAlb Candida albicans (fungi) Dikarya; Ascomycota MLKPNVAIIVAALKPALGIGYKGKMPWRLRKEIRYFKDVTTRTTKPNTRNAVIMGRKTWESIPQKFRPLPDRLNIILSRSYENEIIDDNIIHASSIESSLNLVSDVERVFIIGGAEIYNELINNSLVSHLLITEIEHPSPESIEMDTFLKFPLESWTKQPKSELQKFVGDTVLEDDIKEGDFTYNYTLWTRK >DHFR_canGla Candida glabrata (fungi) Dikarya; Ascomycota MSKVPVVGIVAALLPEMGIGFQGNLPWRLAKEMKYFREVTTLTNDNSKQNVVIMGRKTWESIPQKFRPLPKRINVVVSRSFDGELRKVEDGIYHSNSLRNCLTALQSSLANENKIERIYIIGGGEIYRQSMDLADHWLITKIMPLPETTIPQMDTFLQKQELEQRFYDNSDKLVDFLPSSIQLEGRLTSQEWNGELVKGLPVQEKGYQFYFTLYTKK >DHFR_pneCar Pneumocystis carinii (fungi) P16184 Dikarya; Ascomycota MNQQKSLTLIVALTTSYGIGRSNSLPWKLKKEISYFKRVTSFVPTFDSFESMNVVLMGRKTWESIPLQFRPLKGRINVVITRNESLDLGNGIHSAKSLDHALELLYRTYGSESSVQINRIFVIGGAQLYKAAMDHPKLDRIMATIIYKDIHCDVFFPLKFRDKEWSSVWKKEKHSDLESWVGTKVPHGKINEDGFDYEFEMWTRDL >DHFR_schSti Scheffersomyces stipitis (fungi) Dikarya; Ascomycota MSEIKPTIAIIVAALKPDLAIGFQGKMPWRLRKEIRYFKDVTTKTSDPSKINAVVMGRKTWDSIPARFRPLPDRINIVLSRSFQNETVDTNVIHANSIANSLSQLQPNVERVFVIGGAEIYNELINDSRVTHLLLTEIENTNEDNQIAVDTFLKFPLYSTDSQWRKQPKSELQKFIGSSITLEDDISEGDLKYNYTLWTRRAG >DHFR_spaPas Spathaspora passalidarum (fungi) Dikarya; Ascomycota MTMTEHKPTISIVVAALKPKYGIGYQGKMPWRLRKEIQYFKNVTSKTTKSNGINAVIMGRKTWESIPKKFRPLPDRINIVLSRSFNNETIDDSVIHADSIENGLKLIKGKNVDKVFIIGGGEIYNSVIGSELVDNLLITEIEHTDPETVPMDTFLKFPEDKWVKQPKSELEKFIGEPVDENITEGDYAYNYTLWTRK >DHFR_lodElo Lodderomyces elongisporus (fungi) Dikarya; Ascomycota MTSEKPTISIIVAALKPSYGIGNKGKLPWRLRKEMAYFKRVTLRTTHTQNAVIMGRKTWESIPVKFRPLPDRLNIVLSRSFANEEQENGVIHANSIGRCVELIKEKNLLKVFIIGGSEIYHAFLREKGLVDYLLITEIEQDVKNAEGKDKEGKTKEEEVKEVEMDTFLKFDTNEWVMGSQEELKEYTGETEIETNVKEGDFTYNYTIWKRK >DHFR_debHan Debaryomyces hansenii (fungi) Dikarya; Ascomycota MTAANGKVTPPIAMIVAALLPELGIGIKGKMPWRLRQEIKYFKNVTTTTKDPNGINAVIMGRKTWESIPTKFRPLPGRLNIVLSRSYVSHGSNDEDIILTNSIENALAKVEEYSKPVEKIFVIGGSELYNKLISHEKVQHLLITEIKSTRPVEVDTWLKFPIYTESSDWTKQTNDDLSKFTGIDNEDVEITEGDFTYKYTYWKKKV >DHFR_meyGui Meyerozyma guilliermondii (fungi) Dikarya; Ascomycota MRSVWHRARIYLNHLLPRAFMRQPPIALIVAALLPDLGIGFGGALPWRLKQEIKYFRDVTSNAPDGSINAVIMGRRTWESIPPRFRPLPNRINVVLSRSNPNLEENDVFWGNSFDTALEFLQKRHDINKIFVIGGAEIYNQVINDPRISHLLLTEVSANYDATIPMDRFLHFPREAWTRSPHSQLIQFTGIDATDSTIKEGDFSYNYSLWCKK >DHFR_milFar Millerozyma farinosa (fungi) Dikarya; Ascomycota MTSSGHSLGRGGIPIVMIVAALVPEMGIGLKGKMPWRLKNEMAYFRKVTTTTTDNEAINAVIMGRKTWQSIPEKFRPLPKRLNIVLSRSQHENDGDDAVLFCTSLEEALSKAKKYSKPVEKIFIMGGGELYNQAYNSGQVGHLLLTEIRANKEVETDTRIEFPVYGDQSTWIRQPHSALQELVGGEVEEQQLQEGDFTYGFAYFKKKESDE >DHFR_claLus Clavispora lusitaniae (fungi) Dikarya; Ascomycota MFSCEPGRSLFISHGCLIISARVSSTWLHHIHMIKSLKPVTSVIVAALSPKYGIGAQGKLPWRLKQEMKYFKDVTSAARAGSINAVVMGRKTWESIPKKFRPLPNRLNIVLSRSFSNEEKDGVLYFNSIDSIMSNLAQSNYWYHDKPIDKIFIIGGAEIYNSVMKGDLVDNLLVTNIRYVGNPEAEPVLDTFLDWDMSLWEQSNVSRIREFSDVEFEEGIIKEGDYEYEYTMWERRK >DHFR_komPas Komagataella pastoris (fungi) Dikarya; Ascomycota MSRPFKVNIIVAALIPGHGIGLKGKLPWSLKNEMKYFRLLTTNTISPTSKNVVIMGRNTWESIPSRFRPLQNRLNIVLTRNANISVENGAAVDDSVLVADSLKNGLDIIEKTGNIEEVFLIGGADLYNQCLASGLVDRIFLTEVKSLNGQIETDVSIKIDPSQWIKQESQVLEKYLQDHGVNGFQLDGNKEGELVYDFTLLERR >DHFR_ogaPar Ogataea parapolymorpha (fungi) Dikarya; Ascomycota MTKVSLIVAALVPKYGIGYKGQLPWALKEEMRYFRRVTTQTADKNKKNAVVMGRKTWESIPERFRPLKGRVNVVLTRDLSAFSSKYSEEVAKHGNNVKVADSLKSALQTLDMDNIEEVFVIGGAELYNEVLRTTPELVDRLLLTEVSTEKELEMDAFINVGTLWKKDDPQVWKSYLASKGLENEFSQDNREADFQFSYHIYSRC >DHFR_rhiDel Rhizopus delemar (basal fungus) EIE80550 'zygomycetes' MTQRKLALIAAATEELGIGRKGDLPWHLPRDLRYFRDVTTKIPKGNSAQNVVIMGRVTWESIPPKYRPLDNRFNIVVSRNPNYDLQTTDPNAILVTSFEEALEKIDATKTNPRVFVVGGAQLYSHAIKRADCTDILLTKIKAKIECDAFFPKIDEHVYRPSTHEELEAYVEESVPEGIQTYKDLEYEFTYYKRMV
>DHFR_encHel Encephalitozoon hellem (microsporidia) CP002713 tandem DHFR-TS MFALIVAFAKNKGIGKNNRLPWPPLRTDMSWFRTLSQSVPLITSDRIDLSPSSSNAVVMGRKTWDSIPPRFQPLPNRTNIVLSRRPGISTENTLFISAFGDIEHLRIPSSSMVFVIGGHDIYSLAIQSGRAHIIFATEISESLECDVFFPRIDWDAYERRDITREVSGLVGKDLADAFYNTEANAFTENGITFKMFIYIKHGAL >DHFR_encRom Encephalitozoon romaleae (microsporidia) CP003518 tandem DHFR-TS MFALIVALARNKGIGRANRLPWSRPLRTDMSWFRTLSQSIPLITSHHIDLLPSPSNAVVMGRKTWESIPPKFRPLPNRMNIVLSRRPGAKTENAFFIPTFEDLSHLDIPPSSMIFVIGGHDIYNLAIQNGKAQIMFVTEVFESPDCDVFFPHVDWESYEKRDITYEVSRLIDQNLADAFYNTEANAFTENGTTFKMFIYIKPGSL >DHFR_encCun Encephalitozoon cuniculi (microsporidia) CP003518 tandem DHFR-TS MLALVVALASHRGIGNANALPWPRPLAADMAWFRTLSQSIPLISPDRIALAPSASNAVVMGRRTWDSIPSRFRPLANRINVVLSRGPARSTENTFFIQTFEALDSLPLPPSSMTFVIGGRDVYSLALQSGRPHLIFATEVFESPECDVFFPHIDWASYEKRDITRDVSRLIDRTLASAFYSPETATFTENGTSFKMFLYTKPETR >DHFR_encInt Encephalitozoon intestinalis (microsporidia) NC_014415 tandem DHFR-TS MIALIVALAANQGIGRHGKLPWKHPLKTDMAWFRILSQSVPILSPDHISLSPSKSNVVVMGRKTWHSIPSRFRPLQNRINVVLSRSSLPHSQNTFFIPSFAALDHLPLPPSPATFVIGGHDIYALAIQTGRVQAMFVTEVHESPECDVFFPQVDWSSYQKRDITRDVARLVDTTLVDAFYIPEENIFNEGGISFKMFIYTKLHP >DHFR_nemSpp Nematocida spp (microsporidia) EHY66023 MLPVVLAAYSEGNGVIGREGRLPWPSIKVDFKFMKYLTTKYPSGLIMGRLTYESIGRPLPKRTSIVITSHDKENIQDINYSVLFCKSLESAISICRSLSLQPIIFGGAAVYKEALERYKCKLYLTEIYKIFQGDAFFPLHCIDHRSLVNITPDVLKELSIPENLHRNNVERPLEQSEIYLTQDGRHIPLINTLYENGINYAFLTGYSTPLSEK
>DHFR_harCan Hartmannella cantabrigiensis (slime mold) AAQ02617 Amoebozoa; Tubulinea; Euamoebida pubmed 21112814 MNGFALVVAVTKTMGIGNKGGLPWSSIRGDMNFFKKITSETKDAAKQNAVVMGRKTYVGIPAKFRPLSNRVNIVISTNANLRKEESIPDSVHIVDSFDLAVTKAYNLPNIENVFVIGGSSVYAEAMKHPQCKTIYYTNILTPDFTCDTFFPKIEESVFKVTSRSDLQQEGETSYEFLTYYRI >DHFR_polPal Polysphondylium pallidum (slime mold) EFA83570 Amoebozoa; Mycetozoa; Dictyosteliida MVKISFVVAVSLNNVIGKDGGIPWRLKGDLAFFSKTTTGHPIVMGRKTFDSIGRPLPNRHSIVITSSGKLYNRDGIEYQEKPDVLTIVRSTDQAIELVNNLNSDVVYIIGGETIYKETFKYANELLITTVDTVIEGGDTFFNLFPNKATPLIDCNNQSWVIMDKKSFKKDNDNEYDYTISTLKRE >DHFR_dicDis Dictyostelium discoideum (slime mold) XP_637661 Amoebozoa; Mycetozoa MKVSIIVAVSKNNVIGTKAGDIPWELPKDLKHFRDTTHGFPCIIGRISLEAFGDLLPNRFNIIVSSKKEFINIREKIAINLPEYETGITEKGAQYVIVKSVKEAVDYAKDILKTDLVYICGGKGIYEDSQQYSHELIITDVDVHIEENDVVIYNPFPSNDERNKWKVVNQIHHPKDHQNNLDFSITYYVKNEEILIDYKVENQN
Plant, water mold, stramenopile, alveolata, euglenozoa, and heterolobosea reference sequences
These species have a fused DHFR-TS proteins that additionally form a dimer (rather than the usual DHFR monomer). This fusion is sometimes taken as a decisive rare genetic event that unites plants with all early diverging eukaryotes. However, a fusions of two genes that act sequentially in metabolism is a very poor choice of synapomorphy because the event could have occurred on multiple occasions as selectively advantageous convergent evolution. For example, much-studied fusions in the fungal tryptophan biosynthetic pathway do not seem phylogenetically informative.
Gene fusions may also be readily reversible -- the long linker region need simply develop a stop codon and initial methionine because the upstream promotor can serve for a polycistronic mRNA. This could be tested experimentally but has not. As noted above, microsporidia have adjacent DHFR-TS separated by a few dozen basepairs.
Finally, a single rare genetic event is unacceptable (the minimal accepted standard is three); rare genetic events have to be comprehensively discovered and checked for phylogenetic consistency.
A careful crystallographic study in Cryptosporidium hominis in comparison to Plasmodium falciparum and Leishmania major, suggested the linker domain in fused DHFR-TS could be used in the phylogenetic classification of protozoa, as the linker seems to control the relative orientation of the two proteins. In particular, a longer leader peptide with very short and conserved linker RN seemed informative.
However as more genomes were sequenced in early diverging protozoa, support for this concept completely evaporates. The set of sequences below show no primary sequence relationships in the linker region that correlate with generally accepted classification. In particular, linker regions are evolving way to fast relative to very old divergences -- linkers are not alignable even within the same genus.
The sequences below are broken into two regions: the DHFR and linker, followed by the earliest conserved residues associated with thymidilate synthase. Fused DHFR have limited relevance to the evolution of non-fused DHFR because interactions of subunits within the fused dimer provide selective constraints that have no counterpart in non-fused DHFR.
DHFR has a great many more insertions than TS in these organisms making alignment more problematic. The reason for this is not clear -- some of these may be gene model artefacts (introns not fully removed) and others may be attributable to loops in certain places that can accommodate insertions without affecting functionality. Many of the species studied are obligate parasites; some have highly reduced genomes and anomalous AT composition. At face value however, DHFR (and many other proteins) are evolving quite rapidly in these species, even allowing for the immense time since divergence from last common ancestor with metazoans.
>DHFR_araTha Arabidopsis thaliana (thale cress) Q05763 Viridiplantae; Streptophyta bifunctional DHFR-TS MTSKPQSTYQVVVAATKEMGIGKDGKLPWNLPTDLKFFKDLTLSTSDSAKKNAVVMGRKTWESIPKKYRPLSGRLNVVLSRSSGFDIANTENVVTCSSIDSALDLLAAPPFSLSIEKVFVIGGGDILREALNKPSCEAIHITEIDTSIDCDTFIPTVDTSAYQPWCSSFPICENGLRFSFTTHVRVKSSSAGEASDESDGSKVLQVDWKKFSSVLPKMIFDR HEEYLYLNLVKEIISNGNLKDDRTGTGTLSKFGCQMKFNLRRNFPLLTTKRVFWRGVVEELLWFISGSTNAKVLQEKGIRIWDGNASRAYLDGIGLTEREEGDLGPVYGFQWRHFGAKYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIIMSAWNPSDLKLMALPPCHMFAQFYVANGELSCQMYQRSADMGLGVPFNIASYSLLTCILAHVCDLVPGDFIHVIGDAHVYKNHVRPLQEQLENPPKPFPVLKINPEKKDIDSFVADDFELIGYDPHKKIDMKMAV >DHFR_popTri Populus trichocarpa (cottonwood) XP_002306118 Viridiplantae; Streptophyta bifunctional DHFR-TS MSGDSVANLSNGNANTQQSDPRKTYQVVVAATKDMGIGKDGKLPWKLPSDLKFFKDLTLTTMDSGKKNAVIMGRKTWESIPLEHRPLPGRLNVVLTRSGSFDIATAENVVICGSMGSALELLAASPYCLSIEKVFVIGGGQILRESLNAPGCDAIHITEIETDFDCDTFIPAIDTSVFQPWYSSFPKVENDIRHCFTTYVRVRSSTIESHSQNNGENSDGNSENSKFEVKKFSFLPKMVFER HEEYLYLRMVQDIISDGNLKDDRTGTGTLSKFGCQMRFNLRKNFPLLTTKKVFWRGVVEELLWFISGSTSAKVLQEKGIHIWDGNASREYLDSIGLKDREEGDLGPVYGFQWRHFGARYTDMHADYTGQGFDQLLDVIDKIKNNPNDRRIIISAWNPSDLKLMALPPCHMFAQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTCIIAHVCDLIPGDFVHVIGDAHVYHTHIRPLQEQLRKLPKPFPILKINSEKKDIDTFVAADFKLIGYDPHQKIEMKMAV >DHFR_selMoe Selaginella moellendorffii (spikemoss) XP_002963028 Viridiplantae; Streptophyta bifunctional DHFR-TS MVLDGTPPPPAAAATLRAFQVVVAATRDLGIGKEGKLPWSVPSDMAFFKRITSEAADARKKNAVVMGRRTWESIPAKFRPLPGRVNVVLSRAGLELGDKAIVCSSLDSALGILAAPPFDSSVEAVFVIGGGEVFREAMVSPLCTAIHLTEIQTFFECDTFMPALDLTCFRLWYASSPATVNNVRHSFHTYVRNKALDVRNVAGTCSLPIPEFVRER HEEYQYLNLIKDIIETGNTRSDRTGTGTISKFGCQMRFNLRNSFPLLTTKRVFWRGVVEELLWFISGSTNAKVLEDKGIHIWKGNGSREYLDSIGLSEREENDLGPVYGFQWRHFGARYSTMHDDYTGQGVDQLAEVIDKIKNKPDDRRIVLSSWNPADLKQMALPPCHMFAQFYVSNGELSCQMYQRSCDMGLGVPFNIASYALLTCMIAHVCDLAPGDFVHVLGDAHVYTNHVDPLKEQLENVPKPFPILKIATSNKDIDSFTAKDFQLVDYESHQRIAMKMAL >DHFR_phyPat Physcomitrella patens (moss) XP_001758934 Viridiplantae; Streptophyta bifunctional DHFR-TS pmid 18079367 MASSGSPAPECDEGEQILSGTHSEKLRGFQIVVAATRERGIGKQGHLPWKLPTDMKFFKTVTSVTTSSSKKNAVIMGRHTWESIPEKFRPLPGRLNVILTRSGIKSTPAGVVVSESLQSALALIATPSYSSHVESVFVIGGGQLVSTVCSEAMASPLCEVIHLTEVEGDVECDTYMPAVDTDIFRIWSASIPIVENGLRISFLTYVRGSLTSSIKQTDNGETTVGLLPKYLLEA HDEYQYLNLIDDIIKTGAVKGDRTGTGTISKFGCQMRFNLRKSFPLLTTKRVFWRGVVEELLWFISGSTNAKVLHDKGVKIWDGNGSREYLDKQGLTEREEGDLGPVYGFQWRHFGAKYVDMHADYTGQGYDQLKDVINKIKTDPDDRRILLSAWNPADLKLMALPPCHMFAQFYVANGELSCQMYQRSCDMGLGVPFNIASYALLTCILAHVCDLVPGDFVHVLGDAHVYKNHVEPLQEQLQNTPMPFPRLRIKTANRDIDSFVAADFELIGYKAHQKINMIMAV >DHFR_ostTau Ostreococcus tauri (green alga) XP_003074642 Viridiplantae; Chlorophyta trifunctional dehydrogenase-DHFR-TS smallest free-living eukaryote MASYYRALAFALAGARRYGRDGYEAADLEPLREVQNLVGTAFVVTGGSRGIGFAVSEGLIRRGARVHVVCRDEATGASARERLNALGTRTGGSCEVHVSDLSSLAQTRGLVEKLASRGEALHGLVCNAGVMRRERTTSSEGYExxxxxxxDGASRGPQKGPRVTPDASRGLILKSAPAELDGDAVPTSTSTPGRATSSARRLAWGLARGIDALGAFARAGGRSSTGGGGSATRKVRCASARAIGEAKRSRGG MEMRAFQVVVAATVDGGIGKDNGLPWKLTKDMGHFKKLTSETRDANAVNAVVMGRKTWESIPAKFRPLPGRLNVVLSRSGVLAEANDENNNGAETLPEGVLVRKTIDDALSAISSSEKRVEKTFVIGGAQIYEEALKSERCEAVHLTEVEGDFECDVFIPKIDADKFKLYGQSKPITENGARYQFLTYVTKDSESGKFRPIAGELLPAGCDVK HEEHQYLDMIREIIDQGAVKGDRTGTGTISTFGNQMRFDLRRSFPLLTTKRVFWRGVAEELLWFVAGETNANKLAEKKINIWDGNGSREYLDSIGLTEREVGDLGPVYGFQWRHFGAEYTNMHADYTGKGVDQLAEVINKIKNNPTDRRILLTAWNPAALKEMALPPCHMFCQFYVANGELSCQMYQRSCDMGLGVPFNIASYSLLTCMIAQVCDLKPGDFVHVCGDTHVYSNHVEPLKKQLTCEPRPFPILKINPEKKDIDSFTFDDFEIIGYDPHPKIEMKMAV >DHFR_micPus Micromonas pusilla (green alga) XP_002502546 Viridiplantae; Chlorophyta bifunctional DHFR-TS MASEASTVRPFQVVVAATKGEMGIGYQGGLPWNLPKDMAYFKAVTSQVNEPGTKNAVVMGRKTWESIPAKFRPLPGRVNVVLSRGHTLVEAGSENSAPVNGGGKPEVLPEGVFLRPSLEAALELLSAPEHEAGVEKVFVIGGAQVYAEAMASPHCQALHLTEVTPPADEPEKYKCDAFLPQIDPAKFKLYASAKPLREKDGATIQFLTYFGVDPGTGKFRSPGSKVLPAGAVAKGVR HEEMQYLDLIKEIMEEGNVKGDRTGTGTISKFGCQMRFDLRRSFPLLTTKRVFWRGVAEELIWFVKGCTSAKELQDKDIHIWDGNGSREYLDSVGLGHREEGDLGPVYGFQWRHFGAEYKDMHADYTGQGVDQLAEVIDKIKNNPNDRRILLTAWNPAALKEMALPPCHMFCQFYVANGELSCQMYQRSCDMGLGVPFNIASYSLLTCMVAQVCGLKPGDFVHCCGDTHVYSNHVEPLYKQLENEPRPFPTLKINPEKKDIDSFEFSDFEIVDYDPHPKIAMQMAV >DHFR_chlVar Chlorella variabilis (green alga) EFN56442 Viridiplantae; Chlorophyta bifunctional DHFR-TS MTPEQARRTIQIVVAATKQWGIGKGGSLPWSLPGDMKYFRELTSRTADPAKQNVVIMGRKTWESIPAKFRPLAGRINVVLTRGAVAGDENASAPGNATAALAEASKAEGVHISSSLDSALEMLSGPEFDSRVESVFVIGGGQVYKECMESPLLSAIHLTLVEGEAAEAANCDTFMPPVDESRFRLWSASAPRSVGGTRYSFLCYTRAGQEGLPALPPTVASR HEEHQYLEMIEDVMANGVFRGDRTGTGTYSKFGATMRFNLRHSFPLLTTKRVFWRGVAEELLWFVSGCTNARVLMDKGIHIWDGNGSREYLDSIGLSHREEMDLGPVYGFQWRHFGAEYTDMHADYGGKGVDQLAELIHKIKTNPNDRRLVLSAWNPAALTDMALPPCHMFCQFYVADGELSCQMYQRSCDLGLGVPFNIASYSLLTCMVAQVCGLRPGDFVHVLGDAHVYANHVGPLRDQLKNAPRHFPRLLINPAKTDIDSFVFDDFQLEGYQPHKAIKMQMAV >DHFR_volCar Volvox carteri (green alga) XP_002955893 Viridiplantae; Chlorophyta bifunctional DHFR-TS MTSHKGFQLVVAATPSLGIGKNGTLPGWKLPGDMAYFRELTSRTREPSCQNVAIMGRKTWESIPNKFRPLKGRINIIARRNGAQFGPDVLGCSSLGAALELLEDEKLKRDVDFVFVIGGAQVYAEALRHPSCTAVHLTKVEKEFDCDAHLPPLDPSVFGVWSASEPFQENDTRYSFVCYTRRGLSPPPELPPGMASR HDEIQYLELVRELITSGTFRPDRTGTGTYSRFGRTARYNLRHTFPLLTSKRVFWKGVAEELLWFIRGSTNANLLRDKNIHIWDGNSTREYLDSRGLHHREVGDLGPVYGFQWRHFGAAYKDMHTDYTGQGVDQLKAIIEQIRKDPNDRRMIMSAWNPAALNEMALPPCHMFCQFYVADGELSCLMYQRSCDVGLGVPFNIASYALLTRLVAQVTGLRAGDFIHVMGDTHVYANHVEPLKEQLKNTPRHFPILNINPAKTDIDSFVFEDFELVDYQPHQTIKMKMAV >DHFR_chlRei Chlamydomonas reinhardtii (green alga) XP_001700389 Viridiplantae; Chlorophyta bifunctional DHFR-TS MDTSRKGFQLVIAATPSLGIGKNGKLPDWQLPGDMAYFKELTSRTRDGAHTNAVIMGRKTWESIPAKFRPLKGRLNIVLSRSFADDADLLESALSLLEHKELKGGVEGVFIIGGGQVYAEALAHPNCTAVHLTQVEKEYECDTFLPALDPAVWGVWSSSEPVTENNTRYSFVCYTRRGTEQAPELPPCMASR HDEQQYLELVRELITSGVFRPDRTGTGTYSRFGRTSRYNLRHTFPLLTSKRVFWKGVAEELLWFISGATNANLLRDKNIHIWDGNSSREFLDGRGLGHREVGDLGPVYGFQWRHFGAEYKDMHTDYTGKGVDQLRYIIDTLKKDPNDRRMVMSAWNPAALAEMALPPCHMFCQFYVADGELSCLMYQRSCDVGLGVPFNIASYALLTRLVAQVTGLRAGELVHVMGDTHVYANHVEPLREQLKNTPRHFPTLRINPDKKDIDSFVFEDFELVDYNPHKTIKMQMAV
>DHFR_phyInf Phytophthora infestans (water mold) XP_002904741 stramenopiles; Oomycetes bifunctional DHFR-TS VLCKLSWSPSLVGVVYLVKADEGA* MTEQSIRVVVAALETTGGIGLRQHIPWRLPSDMKHFRALTTASPDSSVQHAVIMGRKTWESLPAKVRPMPKRYNVVLTRDSSYRNKQEIPDTVGVAASFREALKLVQEQGDKVDQVFVIGGGAVYAEALSYPSCTKVHLTRVKGQFECDAFFPLEQLEQNFKVAEESEVKEENGVQFHFVEWERKTGEVKAVESALLVDNTTP HEEMQYLDLIRKILTQGAKREDRTGTGTLSVFGAQMRFSLRNNVFPLLTTKRVFWRGVAEELLWFISGDTSAHTLQQKDIHIWDGNGSREYLDSRGLQHREVGDLGPVYGFQWRHFGAKYTDMHADYTGKGVDQLAEVIHKLRTNPTDRRIVLSAWNPSDLNEMALPPCHMFCQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTRLVAQVTGLKPGEFIHVIGDAHVYLNHVEPLQKQLTRTPCPFPTLHINPEKTSSIDEFTFEDFEWVSDYAGSAIkmkmai >DHFR_albLai Albugo laibachii (white rust) CCA17727 stramenopiles; Oomycetes bifunctional DHFR-TS MVRTISIVVAATEDCWGIGWRQSIPWKLAKDLKHFRDLTTRTFSFNGCNSQNMRNAVIMGRKTWESLPPSYQPLPNRYNHILTRTTNYRIDHSVPHDVGISSSLADALEEIERQEDIGRVFIIGGQKVYEAALECTSCDRIYLTTVKATYQCDTFFPSNLKDRGFQLVSESDEMEENEIRFHFRELSRPIVTITKHSGLYTNEKP HEEYQYLNLIRNIVANGVEKQDRTGVGTKSIFGVQMRFNLRNDMFPLLTTKKVFWRGVAEELLWFISGNTNAKTLQDRKVRIWDGNGSSAYLKSIGLHHREEGDLGPIYGFQWRHFGAKYTDMHADYTGQGFDQLADVILKLKTDPNGRRIILSAWNPNDLNEMALPPCHLMCQFYVAKGQLSCQMYQRSADMGLGVPFNIASYALLTKMIAHVTYLTPGEFVHVIGDAHIYLNHLEPLKLQLERTPRPFPTLLIRPRNDLRTIDDFEFTDFELQNYEPYMAIKMAMAV >DHFR_blaHom Blastocystis hominis (protozoan) CBK20227 stramenopiles; Blastocystis bifunctional DHFR-TS WSVCLNQWFI* MSIIRFSIVAAMTTKRGIGLNGGLPWRIKQDMKFFVDLTTTTTDSEKQNAVIIGKNTYFSFPEKFRPLKNRINFIISHDKELREKHNIPDSVYICSSVDEALILMQEEELKRKIENIFVIGGGQIYKKAINLPECEKLYLTEVDADISCDTFFPEIPLAYKKTKESETFEENNFKFRFAEYTRLPMTFLPPYRTSLQE HEELQYLNLIRYILDHGALRTDRTNTGTRSVFGTQMRFLLANGVIPLLTTKKVFWRGIVEELLWFIRGCTDGRVLKEKNIHIWDGNGTRSFLDSVGLTQNEEDDLGPIYGFQWRHFGAAYEGRDKDYSNQGVDQLKEVIHLLKTDPYNRRIILSAWNPADLRKMALPPCHVMSQFYVQNGYLSCQMYQRSADMGLGVPFNIASYSLLTILLAHITGLKPGEFVHSIGDTHVYVNHEEALLQQIEREPRPFPTLRITKQSENIEEYTFADFELVGYNPHETLKMKMAI >DHFR_ectSil Ectocarpus siliculosus (brown alga) CBN76613 stramenopiles; Phaeophyceae bifunctional DHFR-TS MASSVAVASPSTSRSFVVIVAATAGSLGIGKNGALPWRLAADMAYFKRCTSTPTTSSGTTTATTTTDKINAVIMGRKTWQSIPERFRPLAGRRNVVLSRNPAARDTLRLPKDVLLAGSLTEALDLLSPAAASEVDKVFVIGGGSVYAEAVASELCEKVLLTSVTPPADGRFDDCDTHFPALDPDTFKLVKKGEAQEEKGVELSFDEYESVNHSEAAAAMAAAEGDKENASAASVVGSKRKSSPDGDQVAATGVETPAAAAAAGDGGATADAAAAAGSASTAPVPQEEAEGGPGN EEEMQYLNLVEDILDNGVRRGDRTGTGTLSKFGVQMRFSLREERFPLLTTKRVFWRGVAEELLWFVAGCTNANVLKERGIHIWDGNGSREFLDGLGLTEREEGDLGPVYGFQWRHFGAEYTDMHADYTGKGVDQLAECIQKIKTNPEDRRIVLSAWNPADLGKMALPPCHMFCQFYVAEGELSCQMYQRSADMGLGVPFNIASYALLTRLVAQACGLKAGDFVHTIGDAHVYVNHVDALREQLKRKPRPFPTLSINTDETDIDSFEYKHLEVKGYSPYKTIKMKMAV >DHFR_aurAno Aureococcus anophagefferens (microalga) EGB04584 stramenopiles; Pelagophyceae bifunctional DHFR-TS MRPALTKMAPTNDLPQLTCIVAAVAGSYGIGKDGTLPWKLAGDMKYFKKVTSAAPEGKTNACVMGRKTWLSIPPKFRPLGGRKNVVLSRNPNAREELGLPPDVFVCPSLDAAVDALAGEADLGEVFVIGGGQVYASALADARCTKVLLTSIDDAAGAFADCDAVFPDPAKAGFASAASGEALVENGVAYRFETLTRPAKKPPLRESNGPPAPPPANEKPAAPN HEEKQYLDLIAEILDRGVRRGDRTGTGTISIFGARMSFSLRNDRFPLLTTKRVFWRGVAEELVWFVSGSTNGKLLADKGIHIWDGNGSREFLDRRGLGHREEMDLGPVYGFQWRHFGAKYVDMHKDYAGEGVDQLAACVETIKQDPNSRRIVLSAWNPADLDEMALPPCHMFCQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTRMVAQATGLKPGDFVHVIGDAHVYSNHVDALKVQLQREPRPFPTLTINPAKTGIDDFEFADFTVEGYKPYPKIDMKMAV >DHFR_phaTri Phaeodactylum tricornutum (diatom) XP_002177415 stramenopiles; Bacillariophyta bifunctional DHFR-TS frag VVAAAASHRGIGYQGSLPWRLPGDLRHFQAVTTQPPETGGTNAVIMGRKTWDSIPDRFRPLPGRINVVLTRHPERTLWPDGVLTATSLADAAALLAQPTRAVGHVFVIGGGQVYEQALREKFVNRVVYTEVANLPADSKFDAFFPPLPDAEWQAQPWQTSTTATNKENGNAPETGAWHTDGPSGITYRFLEYTRKVASVTVQPPTDGN AEERQYLDLCRHILDHGVRRGDRTGTGTLSQFGTQMRFSLRDGTLPLLTTKRVFWRGVAEELLWFVAGNTNANDLAAKDIRIWDGNGSRDFLDSRGLGHREVGDLGPVYGFQWRHFGATYGTMHDDYTGKGVDQLAECIDKIKNNPEDRRIILSAWNPADLELMALPPCHMFCQFYVRTASLSCQMYQRSADMGLGVPFNIASYALLTHMIAHVTGRKPGDFVHTIGDAHVYLNHVDALQTQLERTPRAFPKLQINPDKQNIDDFVFADFTVVGYQPHKTIKMEMAV >DHFR_thaPse Thalassiosira pseudonana (diatom) XP_002295808 stramenopiles; Bacillariophyta bifunctional DHFR-TS VVAAAAGSRGIGHQGKLPWRLPGDMNHFKKVTTTPPSPGLTNAVIMGRKTWDSIPSKFRPLDGRVNVILSRKSAAGVEGVPMSTDDNNNNNNAEDVLVASSLEEAMQKLDGRANHGSTFIIGGGEIYSQGIKSGLVKRVIYTNVKGLAEDAKLDAFFPELTDSEWECVPFTPKDNAENEETENNHVSKKAKVAEEHEDAKSGLKYEFLEYIRRDNVDIPEGADVNPEEMQYLEICRDIIENGVRRGDRTGTGTLSKFGVQMRYSLRDNTLPLLTTKRTFWRGVAEELLWFVKGSTNANELADKNIHIWDGNGSREFLDSRGLQHREEGDLGPVYGFQWRHFGAEYKDMHADYTGQGVDQLADCIDKIINNPEDRRIVMSAWNPKDLDVMALPPCHMFCQFYVDTDRNEVSCQMYQRSADMGLGVPFNIASYALLTHMIAKVTGRKAGDFVHTIGDAHVYLNHVDALKEQLERKPRAFPKLKMKDGKDFNDIDGFEFDDFEVVGYKPHKTIKMKMAV
>DHFR_cryHom Cryptosporidium hominis (protozoan) 1QZF Alveolata; Apicomplexa bifunctional DHFR-TS 11 aa linker MSEKNVSIVVAASVLSSGIGINGQLPWSISEDLKFFSKITNNKCDSNKKNALIMGRKTWDSIGRRPLKNRIIVVISSSLPQDEADPNVVVFRNLEDSIENLMNDDSIENIFVCGGESIYRDALKDNFVDRIYLTRVALEDIEFDTYFPEIPETFLPVYMSQTFCTKNISYDFMIFEKQEKKTLQNCDPARGQLKSIDDTVDLLGEIFGIRKMGNRHKFPKEEIYNTPSIRFGRE HYEFQYLDLLSRVLENGAYRENRTGISTYSIFGQMMRFDMRESFPLLTTKKVAIRSIFEELIWFIKGDTNGNHLIEKKVYIWSGNGSKEYLERIGLGHREENDLGPIYGFQWRHYNGEYKTMHDDYTGVGVDQLAKLIETLKNNPKDRRHILTAWNPSALSQMALPPCHVLSQYYVTNDNCLSCNLYQRSCDLGLGSPFNIASYAILTMMLAQVCGYEPGELAIFIGDAHIYENHLTQLKEQLSRTPRPFPQLKFKRKVENIEDFKWEDIELIGYYPYPTIKMDMAV >DHFR_toxGon Toxoplasma gondii (protozoan) Q07422 Alveolata; Apicomplexa bifunctional DHFR-TS MQKPVCLVVAMTPKRGIGINNGLPWPHLTTDFKHFSRVTKTTPEEASRLNGWLPRKFAKTGDSGLPSPSVGKRFNAVVMGRKTWESMPRKFRPLVDRLNIVVSSSLKEEDIAAEKPQAEGQQRVRVCASLPAALSLLEEEYKDSVDQIFVVGGAGLYEAALSLGVASHLYITRVAREFPCDVFFPAFPGDDILSNKSTAAQAAAPAESVFVPFCPELGREKDNEATYRPIFISKTFSDNGVPYDFVVLEKRRKTDDAATAEPSNAMSSLTSTRETTPVHGLQAPSSAAAIAPVLAWMDEEDRKKREQKELIRAVPHVHFRG HEEFQYLDLIADIINNGRTMDDRTGVGVISKFGCTMRYSLDQAFPLLTTKRVFWKGVLEELLWFIRGDTNANHLSEKGVKIWDKNVTREFLDSRNLPHREVGDIGPGYGFQWRHFGAAYKDMHTDYTGQGVDQLKNVIQMLRTNPTDRRMLMTAWNPAALDEMALPPCHLLCQFYVNDQKELSCIMYQRSCDVGLGVPFNIASYSLLTLMVAHVCNLKPKEFIHFMGNTHVYTNHVEALKEQLRREPRPFPIVNILNKERIKEIDDFTAEDFEVVGYVPHGRIQMEMAV >DHFR_babBov Babesia bovis (protozoan) AAP57962 Alveolata; Apicomplexa bifunctional DHFR-TS MSTVYEGCGGLTIYVAIALNRVIGHQNQIPWPHIIHDFRFLRNGTTYIPPEVLSKNPNIQNVVIFGRKTYESIPKASLPLKNRINVIISRTVKEVPGCLVYEDLSTAIRDLRANVPHNKIFILGGSYLYKEVLDNGLCDKIYLTRINKEYPGDTFFPDIPDTFEITAISPTFSTDFVSYDFVIYERKDCKTVFPDPPFDQLLLKGVDIIVPKPKYVACPGVRIRN HEEFQYLDILADVLSHGVLKPNRTGTDAYSKFGYQMRFDLSRSFPLLTTKKVALRSIIEELLWFIKGSTNGNDLLAKNVRIWELNGRREFLDKNGFTEREEHDLGPIYGFQWRHFGAEYLDMHADYTGKGIDQLAEIINRIKTNPNDRRMIVCSWNVSDLKKMALPPCHCFFQFYVSDNKLSCMMHQRSCDLGLGVPFNIASYSILTAMVAQVCGLGLGEFVHNLADAHIYVDHVDAVTTQIARIPYPFPRLRLNPDIKNIEDFTIDDIVVEDYVSHPAIPMAMSA >DHFR_plaFal Plasmodium falciparum (malaria) AAA29585 Alveolata; Apicomplexa bifunctional DHFR-TS MMEQVCDVFDIYAICACCKVESKNEGKKNEVFNNYTFRGLGNKGVLPWKCNSLDMKYFCAVTTYVNESKYEKLKYKRCKYLNKETVDNVNDMPNSKKLQNVVVMGRTNWESIPKKFKPLSNRINVILSRTLKKEDFDEDVYIINKVEDLIVLLGKLNYYKCFIIGGSVVYQEFLEKKLIKKIYFTRINSTYECDVFFPEINENEYQIISVSDVYTSNNTTLDFIIYKKTNNKMLNEQNCIKGEEKNNDMPLKNDDKDTCHMKKLTEFYKNVDKYKINYENDDDDEEEDDFVYFNFNKEKEEKNKNSIHPNDFQIYNSLKYKY HPEYQYLNIIYDIMMNGNKQSDRTGVGVLSKFGYIMKFDLSQYFPLLTTKKLFLRGIIEELLWFIRGETNGNTLLNKNVRIWEANGTREFLDNRKLFHREVNDLGPIYGFQWRHFGAEYTNMYDNYENKGVDQLKNIINLIKNDPTSRRILLCAWNVKDLDQMALPPCHILCQFYVFDGKLSCIMYQRSCDLGLGVPFNIASYSIFTHMIAQVCNLQPAQFIHVLGNAHVYNNHIDSLKIQLNRIPYPFPTLKLNPDIKNIEDFTISDFTIQNYVHHEKISMDMAA >DHFR_thePar Theileria parva (parasitic protozoan) EAN31856 Alveolata; Apicomplexa bifunctional DHFR-TS MAEDYSGLPFVKLLVAITPENGIGISNGLPWPHIKRDFLFMFRATTYVDPEFKEKHPGAQNVIIIGRKTYDSLPEGTFPLKNRINVVISRNLTEVPGGLVFPSMPAAIRHVTEHLNYYKIFIMGIFHLNIKHLGGSYVYRDALDARIVDKMYVTRVNKNYKCEVFFPKIPDDFVITGISKTFSSEDTTYDFVTYERKDLIVDDRVKKTFEEMLLTGEKFATKDQLVKACPSLKVRY SQELQYLEILADILNTGYHKKNRTGVDCIAKFGYQMRFDLSESFPLLTTKKVFLRGIIEELIWFIKGSTNGNELLKKNVRIWELNGARSFLDNLGFFDREEHDLGPVYGFQWRHFGAKYVDMHTDYSNQGIDQLSLVIDRIKNNPDNRRLIVCSWNVADLSKMALPPCHLLFQFFVADGTLSCMMHQRSCDMGLGVPFNIASYSLLTYMIAQICGLKPGEFVHNLGDAHIYNNHIDAIKEQISRVPYPFPTLRLNKDITRIEDFTIDDIKVENYRSHPVIKMEMAA >DHFR_perMar Perkinsus marinus (protozoan) XP_002768310 Alveolata bifunctional DHFR-TS MALPQLSVIVAHTCKWGIGKDGQLPWKSLPEDMKRFKKITTGGHCNDNVKNVCIMGRKTWESIPERFRPLRDRINVVISSTTSPTDYPPGVRICPSLLDSLASLQQGEVEIGEIFVIGGASLITEAMQLPQCRTLYTTRVGTDPWECDVFMEKIDEKIWEPIAVSRTFRHSDIPYDFVDYKRREGSDKTELSLAERCPRGKFQ HGEYEYLRLIEEIIENGEDADDRTGVGTKTIFSCHMRFSLRDGVFPLLTTKRVFWRGVVEELLWFIRGDTNAKHLSDQGVKIWDLNGTREFLDGRGLTHREEGDLGPVYGFQWRHFGADYVDMHADYSKSGGVDQLAEVIRQLKENPMDRRIIMTAWNPTALPLMALPPCHMMCQFYVNSKQELSCAMYQRSADMGLGVPFNIASYALLTCMLAQVCGLKPGEFCHHLGNTHVYNNHIEPLRQQLKRTPRPFPLLKINPNVKTIDDIKSSDFELIGYNPWGKIAMEMAV >DHFR_parTet Paramecium tetraurelia (ciliate) XP_001453677 Alveolata; Ciliophora bifunctional DHFR-TS MTTNKTFSMVLAMTLNGGIGYQNRLPWKLKEDLQRFKKITTGGIVIMGRKTFESMNSKPLPNRVNVVISKNMKSSNEVQVFPRIEDALQFYNTSHQKLYLIGGKRIFEEGLATDKCSDVHLTRIGVETKCDVYLNKNIFSTFQVNKTSQTKSENGINYDYQHLINKNSHEQSYIDEE HQENQYLDMITKIMKEGVSKDDRTGVGTMSIFGQTMRFNLAQSFPLLTTKKVFFRGVVEELLWFLRGNTNGKLLLDKGVKIWEGNGTREYLDTIGLQHRQEHDLGPVYGFQWRHFGAKYKDCQTDYSNQGVDQVKEIIQLLKNNPDSRRIILSAWNPIDLKQMALPPCHVMSQFFVANGKLSCMMYQRSCDFGLGIPFNIASYALLTYMLAKECNLNLGEFVHVLGDTHIYSNHVEALKKQIERVPYPFPLLKIKGNKSLFDYTYEDFELVGYNAHDKIEMKMAV >DHFR_tetThe Tetrahymena thermophila (ciliated protozoan) EAR85731 Alveolata; Ciliophora bifunctional DHFR-TS MKTRHFDIVLAQTLKKQGIGYKNSLPWRLPNELKNFKKITTETKNKGLQNAVIMGKNTWEALPKKQQPLKDRLNIVISTTMQEGQIADHSYACKSLDSALNFLEQQNQIQDALVIGGAKLCQQALSDQRLRQIHLTRVGVEVECDVFMQKDYLKNFDMIEVSETQSENNLNYDFTRYFNKNYKGQVDPSLFKKMYKP HQEYQYLELIDEIIKNGHVKTDRTGTGTISQFGKLMRFDLSKSFPLLTTKNVFWRGVVEELIWFIKGSTNSKILSEKGVKIWDGNGSREFLDQLGFKNREEGDLGPVYGFQWRHFGAEYKDMHTNYKGKGVDQLQDLINTIKKNPDSRRMIMNAWNVKDLPLMALPPCHVMSQFYVNDNKLSCMMYQRSCDMGLGIPFNIASYALLTHMIAQVTNMQVGEFIHVLGDAHVYSNHVDQLKIQLERAPYPFPLLKINNNKQYNSIEDFTLEDFELIGYNYHPKIQMKMAV
>DHFR_tryCru Trypanosoma cruzi (trypanosome) AAX40325 Euglenozoa bifunctional DHFR-TS short RN linker ETVAEGTRLALRAFSLVVAVDEHGGIGDGRSIPWNVPEDMKFFRDLTTKLRGKNVKPSPAKRNAVVMGRKTWDSIPPKFRPLPGRLNVVLSSTLTTQHLLDGLPDEEKRNLHADSIVAVNGGLEQALRLLASPNYTPSIETVYCIGGGSVYAEALRPPCVHLLQAIYRTTIRASESSCSVFFRVPESGTEAAAGIEWQRETISEERTSANGNETKYYFEKLIPRN REEEQYLSLVDRIIREGNVKHDRTGVGTLSIFGAQMRFSLRNNRLPLLTTKRVFWRGVCEELLWFLRGETYAKKLSDKGVHIWDDNGSRAFLDSRGLTEYEEMDLGPVYGFQWRHFGAAYTHHDANYDGQGVDQIKAIVETLKTNPDDRRMLFTAWNPSALPRMALPPCHLLAQFYVSNGELSCMLYQRSCDMGLGVPFNIASYALLTILIAKATGLRPGELVHTLGDAHVYSNHVEPCNEQLKRVPRAFPYLVFRREREFLEDYEEG >DHFR_leiTro Leishmania tropica (trypanosome) AAM88659 Euglenozoa bifunctional DHFR-TS short RN linker MSRAAARFKILMPETKADFAFPSLRAFSIVVALDKQHGIGDGESIPWRVPEDMAFFKDQTTLLRNKKPPTEKKRNAVVMGRKTWESVPVKFRPLKGRLNIVLSSKATVEELLAPLPEEKRAAAEQDVVVVNGGLAEALRLLARPPYCSSIETAYCVGGAQVYADAMLSPCVEKLQEVYLTRIYTTAPACTRFFPFPPENAATAWDLASSQGRRKSEAEGLEFEICKYVPRN HEERQYLELIDRIMKTGIVKEDRTGVGTISLFGAQMRFSLRGNRLPLLTTKRVFWRGVCEELLWFLRGETNAXLLADKDIHIWDGNGSREFLDSRGLTENKEMDLGPVYGFQWRHFGADYKGFEANYDGEGVDQIRFIVETIKANPNDRRLLVTAWNPCALQKMALPPCHLLAQFYVNTDTSELSCMLYQRSCDMGLGVPFNIASYALLTILIAKATGLRPGELVHTLGDAHVYRNHVDALKAQLERVPHAFPTLIFKAERQFLEDYELTDMEVIDYVPHPPIKMEMAL >DHFR_criFas Crithidia fasciculata (trypanosomid parasite) AAA30318 Euglenozoa bifunctional DHFR-TS 1989 MSRAAAKFKIPMPVTKADFAFPSLRAFSIVVAADQQHGIGDGETIPWTVPETLAFFKDQTTLLRNKKPPTEKKRNAVVMGRKTWEVPLKFRPLKGRLNVVLSCRRPVDDLLAQLPEEKRAAAAADVVINGGLKEALHLLARPPYCSSIETAYCIGGARVYTEAMQSPCVEKLKEVYLTRVHTAPTCNRFYEFVRRRRARAAVDLESTSGVKVSDAAAHLSYEIMKYVPHN AEERQYLELIDRIMKTGLVKEDRTGVGTISLFGAQMFSLRDNQLPLLTTKRVFWRGVCEELIWFLRGETNAHVLADKDIHIWDGNGSREFLDSRGLTENKEMDLGPVYGFQWRHFGADYKGFDANYDEGVDQIKTIVETLKTNDRRLLVTAWNPCALHKMAVRPCHLLGQFYVNTQTKELSCMLYQRCCDMGLGVPFNIASYALLTILIAKATGLRPGELVHTLGTAHVYSNHVEALKEQLQRVPVAFPVLVFKKEREFLEDYESTDMEVVDYVPYPPIKMEMAV
>DHFR_naeGru Naegleria gruberi (amoeboflagellate) XP_002668828 Heterolobosea; Schizopyrenida bifunctional DHFR-TS DIIKEl MMQSTSNLPKASSIPPMEAVVAVLLNSNGIGLNGNLPWLQDGLTEDMKHFVSVTCSSEPLKFISPSMYKQSSSSSPITSDNTNDNTTTINDQLMNAVIMGRKTWESIPSKFKPLSKNRHAIILSSKVNDDDQLMMERTTFVKNVDELFELLQNQKFNRIFIAGGTSIYKLLLPYTAVIHYTNIIEQKGVNIQVDTRFPIRLLPSSDYRNVPINSNQFIAVDKSDKILPKNNERISHYEFITFERR HEEFQYLDIIKELILSDINVREERTGVGTVSKFGYQMKYSLRNNVFPLLTTKRVFWKGVAKELFWFISGSTNAKILSEQDIHIWDGNGSREYLDSLGFENREEMDLGPVYGFQWRHWGSSYENMHTDYTNKGVDQLKKCIETLKNNPTNRRIILSAWNVSDLPNMALPPCHLMCQFWVDTDTNELSCQMYQRSCDMGLGVPFNIASYALLTRLMAQVCNLKPGDFIHTLGDTHVYKNHIEPLKKQIERDPKHFPLLLLDPSITNIDDFKFEHLTLQNYEPHATIKMEMAV
Bacterial reference sequences
Bacterial DHFR, especially that of E. coli, have been intensively studied for decades; only a small sample of the available sequences is provided below. Compared to human, the sequences are 26% identical and 38% similar (as defined below); the 3D structures align to a root-mean-square differance of 2.0 angstroms.
The 26% identity is a mix of truly invariant residues important to the fold or active site, probabalistic agreement at reduced alphabet positions, and accidental agreement at unconstrained positions. In view of the trillion generations that E. coli has undergone since divergence with human, the 28% identity probably provides a floor to possible divergence with retention of function. In other words, that level of divergence was reached long ago (testably in the reconstructed Cambrian human ancestal DHFR) and is no longer significantly increasing. However matching E coli with extreme parasitic eukaryotes (ie sister to human within eukaryotes) can give lower matches.
10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 | | | | | | | | | | | | | | | | | | DHFR_homSap MVGSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND DHFR_escCol ---MISLIAALAVDRVIGMENAMPWN-LPADLAWFKRNTLNKPV-------IMGRHTWESI---GRPLPGRKNIILSSQPGTDDRVTWV--KSVDEAIAA------CGDVPEIMVIGGGRVYEQFL--PKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSE---FHDADAQNSHSYCFEILERR- Consensus I A # IG # $PW L # F R T V IMG TW SI RPL GR N !LS # S D#A V ! !!GG VY # $ P KL% T I # E DT FP# # # D # # Y FE! E homSap (187 aa) to escCol (159 aa) Residues conserved (upper-case letters) : 49 or 26.2% Residues not conserved (white space): 118 or 63.1% Residues identical or similar: 38.0% IV conserved positions (!) : 5 or 2.6%; LM conserved positions ($): 2 or 1.1%; FY conserved positions (@): 1 or 0.53%; NDQEBZ conserved positions (#): 5 or 2.62% (B = D/N, Z = E/Q)
>DHFR_escCol Escherichia coli (bacteria) 1DDS gammaproteobacter MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRHTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR >DHFR_salEnt Salmonella enterica MNPESVRIYLVAAMGANRVIGNGPDIPWKIPGEQKIFRRLTESKVVVMGRKTFESIGKPLPNRHTVVLSRQARYSAPGCAVVSTLSQAIAIAAEHGKELYVAGGAEVYALALPHANGVFLSEVHQTFEGDAFFPVLNAAEFEVVSSETIQGTITYTHSVYARRNG >DHFR_klePne Klebsiella pneumoniae MNPELVRIYLVAAMGANRVIGNGPDIPWKIPGEQKIFRRLTEGKVVVMGRKTFESIGKPLPNRRTVVLSRQASYSAAGCAVVSTLSQAIAIAAEHGKELYVAGGAEVYALALPRADGVFLSEVHQTFEGDAFFPVLDEAEFEVVSAETVQATITYTHSVYARRNG >DHFR_halNea Halothiobacillus neapolitanus MNQQAVRIYLVAAMGSNRVIGNGSNIPWRIPGEQKIFRTLTEGKVVVMGRKTFESIGKPLPNRHTVVISRQTHYLAPGCTVATTLAQAIAIAAEAGKDLYVAGGAEIYALAMPHAHGIFLSEVHQTFDGDAFFPKLDEKDFELVSSETIQAAIPYTYSVYERRNG >DHFR_macCas Macrococcus caseolyticus MIISLIAAISSNYVIGKDKDIPWKIPGEQVRFKDLTMGKSVIMGRKTFESIGQPLPNRKTIIISKSKDINYNNCLTVESLERAFNLLQQEDEIFIAGGGEIYKESLPFADRIYLTIIEKEYEGNIFFPMFNKDEFEITYQQKVEGFIPYTYFTYERKNKGEQIK >DHFR_geoUra Geobacter uraniireducens MVISLIAAMAENRVIGRNNAIPWDIPADRKRFRALTLGHPVIMGRKTFESLAGPLPGRKNIIITRQMDYRAEGCFIAHDLASALAMAGETDEIFICGGGEIYRQALPIADRIYLTIIHSRFDGDTRFPEIPERFVEVGRETVENAIPYSFVLYDKRPSSASPA >DHFR_breLat Brevibacillus laterosporus MLSSIFAMGQNRVIGRDNQLPWRLPEDLKYFRRITTGHAIIMGRKTYESIGKPLPNRRNIVLTTQENYKADGCEIAHSIEEVLSLIDHKEEAFIIGGAEIYKLLLPFTSKMYITKIGQEFEGDAFFPAFDESEWALVEVTPGVQNEENPYQYEFQIYVRSVTNCK >DHFR_oxaFor Oxalobacter formigenes MMTISHVVAMAENRVIGKDGQMPWHIPGEQKIFRELTVGKALILGRKTHESIGRVLPDRITIIVSRKKDYEVPGAHVVHDIDEAIRLAREFGKEDIVIGGGGELFSQTLPVTDKVYLSIVHADFDGETFYPELPKEEFVEKARKEIDASIPYAFVEFERVKGK >DHFR_nocSpp Nocardioides spp MTPGGKRVVLVAAVARNGVIGDGPDIPWQLPGEQRLFKGLTWGHILVMGRATYDSIGRPLPGRTTIVLTRSPDWSAEGVHVAHDLPAALALADTLEGEVMVAGGGQVYAAALPVADEQVLSEVDLEPQGDAFYPEFDRSEWRETAREQHDGYDRVVLVRA >DHFR_cloCel Clostridium cellulovorans MISLIVAVAKNNVIGNNGIIPWKIKGEQKRFKELTIGKTIIMGRKSFEEIGKPLPNRKTILISNTQCIESENCTTVNSLSEAFDLTKDEDEVFVAGGGQVYKESFTYADRIYITVIDKIIDGNVYFPEISSDDFIKSYEERIDAEIPYTYYTYERRK >DHFR_marPos Marinomonas posidonica MLSLIVAMSTNRTIGINNSLPWHLPNDLKYFKQATMGKPIVMGRKTFESIGKPLPGRRNIVITRDANYQADGIDVVSSLEQAISLGEDICLVDGQEEVMVIGGAQIYELALPKADRLYITHVDAEVNGDAFFPEVDWPSFTVMAEESFAAEGPNPYDYRFSVYQRTSAE >DHFR_pseAla Pseudoramibacter alactolyticus MIALIAACDRHRLIGDHGRIPWRIPGEQARFQKLTTGHVVIMGRQTYAEIGRPLPRRQTIVLSRNQTFRAPGCRIAASLAEALRLGAAFSDQLFIAGGAAVYSKALPLADVLYLTEIDAAYVGDTYFPAFDPALFTKTLDAHISGDPSYDYYTYLRKS >DHFR_halOch Haliangium ochraceum (bacteria) CP001804 Deltaproteobacteria MSSPSAPPNDTSS- trimmed ASTPADAEHVFDLVVVADLDGGIARKGEIPWHLSADLRHFQQLTRTTAEPAQQNAVIMGRKTWESLPAASRPLPSRRNIVLSRDAELTLPPGVVHAAELEAALAATDLATPPIDRRFIIGGKGVYAEAMQRRGCRHIFYTEIQDRFDCDLFFPAFKDRFKRTDLLAEGEEKGIGYRIEVWTRTGFPGPV >DHFR_braInt Brachyspira intermedia (bacteria) CP002874 Spirochaetes MIVSLIAAVDSKNGIGLNGVMPWGHIKEDMQFFRSTTTGYAVVMGRVTFESLGSKPLPNRKNIVISSSINNDLLEKYDNLFYESSFENTISKLLLEKNNQIFIIGGESIYKKALDYADIIYLTHIDKDYNCDRFFPQIDTKLFQSSKLKTFFHNDININIIKYTRTLL
Archaeal reference sequences
A small sampler of archaeal DHFR sequences is provided below, half of them best-blast to human and half to E coli. These lie entirely within euryarchaeotes; crenarchaeotes do not appear to have a relevant homolog and may use dihydropteridine reductase to conduct the reaction as does the bacterium Thermus thermophilus (which lacks DHFR in its completely sequenced genome). The extreme halophile, euryarchaeon Haloferax volcanii, has two DHFR, one of which is adjacent to but not fused with thymidylate synthase. The best percent identity 29.3% (human DHFR to Haladaptatus paucihalophilus) is significantly higher than any bacterial match to human, consistent with eukaryotes having a closer phylogenetic association with archaea than to bacteria.
10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 | | | | | | | | | | | | | | | | | | DHFR_homSap MVGSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIP-EKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND DHFR_halPau -MTRLVCIAAVAENGVIGRDGDMPWH-YSADLRHFKETTMGHPV-------VMGRTTYESIAGQLDGPLPGRTNVVLSRSNPDLPEEV-VLVHSVEEAIETVEELD-----DTAFVIGGATVYEQFLPHAD--ELILTEIHDEYEGDTHFPEWDPKEW----------TEVERDEREELDFVRYER-- Consensus mgrLnCIaAVa#NggIGr#GD$PWh lra#lRhFqrmTmghpV !MGrkTweSIa #l#rPLkGRiNlVLSRenk#lP#ea fLsrSl##AieltE#l# Dmaf!!GGasVYe#a$nHad eLilTrIh##%EgDThFPEiDleew s#V#r#ereeldFerYEr >DHFR_halPau Haladaptatus paucihalophilus (archaea) ZP_08042706 euryarchaeote MTRLVCIAAVAENGVIGRDGDMPWHYSADLRHFKETTMGHPVVMGRTTYESIAGQLDGPLPGRTNVVLSRSNPDLPEEVVLVHSVEEAIETVEELDDTAFVIGGATVYEQFLPHADELILTEIHDEYEGDTHFPEWDPKEWTEVERDEREELDFVRYER >DHFR_halVol Haloferax volcanii (archaea) YP_003535331 euryarchaeote MELVSVAALAENRVIGRDGELPWPSIPADKKQYRSRIADDPVVLGRTTFESMRDDLPGSAQIVMSRSERSFSVDTAHRAASVEEAVDIAASLDAETAYVIGGAAIYALFQPHLDRMVLSRVPGEYEGDTYYPEWDAAEWELDAETDHEGFTLQEWVRSASSR >DHFR_natPel Natrinema pellirubrum (archaea) ZP_08963336 euryarchaeote MSEDRDVTEAAAALEPARELVGIVAVAENGVIGRDGDMPWHVPADLQHFKETTMGHPVIMGRVTYEGILETLGEPLPGRTTVVLTSRDLETPEGAVVAHDLESALERAETAARERHDDADRIFVAGGATVYEQFLPALDRLIVTEIHDDPEGDTSFPEWDRSEWDVVSRDDRDGFAFLEYVRRD >DHFR_halMed Haloferax mediterranei (archaea) YP_006350571 euryarchaeote MSEHTDDSVRFVLVAAVAENRVIGRDGDMPWHLPEDLKHFKATTMGHPVVMGRTTYESIARQIDGPLPGRRNIVLTSRDLDLPAGAETVDSVDEAVTAAEAAADDMGVETVYVVGGATVYEQFLDRASGLILTELDEAYEGDTYFPEWRDTGDETSWFEVDRDDRDGFSFVEYERRGDVAESEGAR >DHFR_natMag Natrialba magadii (archaea) YP_003481102 euryarchaeote MTVNTGADTDPVVEPDHELVGIVAVADNGVIGKDGDMPWHIPEDLQHFKETTMAHPVIMGRVTYESIVDALGEPLPGRTTVVLTSRDLETPENAVVAHDLQSAVEEAAAAADERHDGADRVFVAGGATVYEQYLPALDRLIVTEVHEEPDGDTQFPDWDRTDFDEVERDEHDGFAFVEYVRTP >DHFR_natPha Natronomonas pharaonis YP_327110 MKLVLIAAVAENGVIGTDGEMPWHYPEDLKRFKETTMGHPVIMGRTTYESITGQLGGPLPGRTNIVLSSRESLPIPEEVVHARDIEAALSAAEDALDADKQTVYVAGGATVYEQCLDRADELRITEVPESPDGDTYFPAIGDEWTATEREAGDEVAFVRYERAGD >DHFR_halLac Halobiforma lacisalsi ZP_09949777 MAGEPNSETISALEAAEATDRELVGIVAVADNGVIGADGEMPWHLPADLAHFKETTMDHPVIMGRVTYEGILETLGDPLPGRTTVVLTSQDLETPENAVVANGLTEAVERADRAARERHDGADRIFVAGGATVYEGFLPVLDRLVVTEIHDDPEGDAYFPAIDPEDWIEADRDERDGFAFVEYVRRD >DHFR_halWal Haloquadratum walsbyi YP_005840239 MNNINSDDRSPTDGSERTHNQTTDHDVDIDIDIDIDIVLIAAVAANDIIGRDGEMPWHIPADLQQFKRRTMGHPVILGRRTYEAIIGALGEPFPGRTSIVLSSQSRDVPADVILVHSITSAIREASKNARERGVGTVYVAGGGGVYEQFLPLADRLRLTELHDEYEGDTKFPSWDDTVWQETTREKNETFDFVTYERTP >DHFR_halBor Halogeometricum borinquense YP_004036472 MNESDSIDRDTTDANAESIEYVLVAAVAENGVIGRDGRMPWHFSEDMAHFKQTTMGHPVILGRKTYENIVDAIGEPFPGRMSIVLSSRELDLPEGAVLANSIKEATERAESVAEEMGVETVYVVGGARVYAQFLPRATRMILTEIHDSYDGETEFPEWEEAMWIETDRDERESFDFVTYERVE >DHFR_halXan Halopiger xanaduensis YP_004597600 MSEDDESTPGAAESALAAETDRELVGIVAVADNGVIGKDGDMPWHIPADLQHFKETTMDCPVIMGRVTYEGILEALGEPLPGRTTVVLTSRDLETPENAVVANDLAEAVEAAETAARERHDGADRAFVAGGATVYEQFLPALDRLIVTEVHDAPDGDTVFPDWDRDAWTETDRDEHDGFAFVEYVRERA
Duplicated DHFRL1 in primates
This gene is a spin-off of the parent DHFR gene that occured in stem primates. As a retrogene, it has lost all introns. In some species including human, it has been reported to be functional though it is unclear how mitochondria accomplished this function prior to the duplication. In other species, this locus has recently pseudogenized leaving only debris; here some function must have persisted for millions of years in view of maintenance by selective pressure. DHFR, being ubiquitously expressed, has given rise to many other retroposed loci; some of these too may be functional.
>DHFRL1_homSap Homo sapiens (human) NM_001195643 synteny +ARL13B -DHFRL1 +NSUN3 chr3 MFLLLNCIVAVSQNMGIGKNGDLPRPPLRNEFRYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLARSLDDALKLTERPELANKVDMIWIVGGSSVYKEAMNHLGHLKLFVTRIMQDFESDTFFSEIDLEKYKLLPEYPGVLSDVQEGKHIKYKFEVCEKDD >DHFRL1_panTro Pan troglodytes (chimp) ti|1986883368 +ARL13B -DHFRL1 +NSUN3 short c-terminal MFLLLNCIVAVSQNMGIGKNGDLPRPPLRNEFRYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLNDRINLVLSRELKEPPQGAHFFARSLDDALKLTERPELANKVDMIWIVGGSSVYKEAMNHLGHLKLFVTRIMQDFESDTFFSEIDLEKYKLLPEYPGVLSDVQEGKHIKYKFEVYEND >DHFRL1_gorGor Gorilla gorilla (gorilla) ti|2051780408/ti|2033585870 10 bp deletion so frameshift; synteny unknown but 97% identical homSap MFLLLNCIVAVSQNMGIGKNGDLPRPPLRNEFRYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLAISLDDALKLTERPKLANKVDMIWIVGGSSVYKEAMNh lKLFVTRIMQDFASDTFFSEIDLEKYKLLPEYPGVLSDVQEgKHIKYKFEAYEKND >DHFRL1_ponAbe Pongo abelii (orangutan) genome/blat +ARL13B -DHFRL1 +NSUN3 MVRLLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLARSLDDALKLTERPELANKVDMIWIVGGSSVYKEAMNHLGHLKLFVTRIMQDFESDTFFSEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND >DHFRL1_nomLeu Nomascus leucogenys (gibbon) ADFV01005477 +ARL13B -DHFRL1 +NSUN3 MVRLLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLEDRINLVLSRELKEPPQGAHFLARSLDDALKLTEQPELANKVDMIWIVGGSSVYKEAMNRSGHLKLFVTRIMQDFESDMFFSEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND >DHFRL1_macMul Macaca mulatta (rhesus) genome/blat +ARL13B -DHFRL1 +NSUN3 MVRSLNCIVAVSQNMGIRKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLARSLDDALKLTEQPELANKVDMIWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFLPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND >DHFRL1_papHam Papio hamadryas (baboon) ti|1986883368 synteny unknown MVHSLNCIVAVSQNMGIGKNGDLPWPPLRNEFGYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLARSLDDALKLTEQPELANKVDMIWIVGGSSVYKEAMNHPGHLKLFLTRIMQDFESDTFLPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKN >DHFRL1_calJac Callithrix jacchus (marmoset) genome/blat frameshift and stop codon; +ARL13B -DHFRL1 +NSUN3 mvrllCIVAVSQNMGIGKKGDLPWPPLRNEFRYFQRMTTTSSIEGKQNLVIMGRKTWFSIPEKNRPLKDRINFVLSRELQEPPQGAHFLARSLDDALNLTEQPELANKVDMIWIVGGSSVYKEAMNRPGHLKLFVTRIMQDFESDTFFPEIDLEK*KLLPEYPGVLSDVQEEKGIKYKFEVYEKND >DHFRL?_otogar Otolemur garnettii (bushbaby) AAQR03025778 synteny unknown MVHRLNCIIAVSQNRGIGKNGDLPWPPLRNEFNYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKESSQGAHFLAKSLDDALKHIEEPELANKVDMVWIVGGSSVYKEAMNQPGHVKLFVTRIMQDFECDTFFPEIDLKKYKLLSEYPGVLSDVQEEKGIKYRFEVYEKND >DHFRL?_micMur Microcebus murinus (lemur) ABDC01288917 synteny unknown MVRTLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQKMTTTSSVEGKQNLVIMGRKTWFSIPEKSRPLKDRINVVLSRELKEPPQGAHFVAKSLDDALKLIEQPELANKVDMVWIVGGSAVYKEAMNQPGHLKLFVTRIMQDFESDTFFPEIDLKKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND >DHFRL??_micMur Microcebus murinus (lemur) ABDC01470170 synteny unknown two stop codons and a frameshift MVHTLNCLVAVSQNMGIGKNGDLPWPPLRNEFKYFQKMTTTSSVEGKQNLVIMGKKTWFSIPEKN*PLKDRINVVLSREL*EPPQGAHFIAKSLDDALKLIEQPELANKVDMVWIVGGSAVYKEAKNQPGHLKLFVTRIMQDFESDTFFPEIDMKKYKLLSECPGVLSDVQEEKGMKYKFEVYERnd >DHFRL?_tupBel Tupaia belangeri (treeshrew) AAPY01242529 several candidates but synteny unknown retrogene shown two frameshifts MVRPLDCIVAVSQmGIGKNGDLPWPPLRNEFKYFQRMTTTSSVEGKQNTVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPRGAHFLAKSLDDALKLIEQPELANQVDMVWIAGGGSVYKEAMNQPGHLKPFVTRIMQDFESDTFfPEIDLEKYKLLPGYPGVLSDDQEEKGIKYKFEVYEEN
Duplicated DHFRL2 in bony vertebrates
This locus is a small segmental duplication of the parent DHFR gene that occured in stem teleosts and persisted through lobe-finned fish and amphibian divergences, only to be lost in all amniotes including human. It has retained all introns and splice junction phases. Ray-finned fish supposedly had a stem whole genome duplication -- had both copies been retained, three diverged copies of DHFR in paralogous blocks would be present today. Coelocanth, a lobe-finned fish, and frog were not subject to this whole genome duplication.
Zebrafish and other ray-finned fish have a second functional copy of DHFR denoted here as DHFRL2. It did not arise from whole genome duplication, because the two loci are utterly different in syntenic context and since the duplicated locus also occurs in coelocanth and two amphibians. The gene tree for DHFRL2 forms a distinct, more diverged cluster that branches off from DHFR in early bony vertebrates.
This feature cannot be a pseudogene because of billions of years of observed branch length conservation. Pseudogenes persist for a few million years at most so quickly become undetectable. DHFRL2 could conceivably be a very recent pseudogene in some species but not all because that requires many separate events which is not parsimonious. DHFRL2 orthologs are currently available for 15 species.
However its function is obscure: it may function in mitochondria like primate DHFRL1, or be specialized to expression in a developmental stage or intra-cellular location, or have a slightly different substrate, or even have a non-catalytic regulatory role.
The main DHFR has syntenic context FAM151B ANKRD34B DHFR MSH3 RASGRF2 whereas the secondary DHFR2 sits in completely different surroundings RFX4 RIC8B SIRT3 C12orf23 CHST1 MTERFD3 DHFRL2 CRY1 BTBD11. Frog, like all rayfinned fish, unmistakably has a DHFR in the first spot and a DHFRL2 in the second. For a whole genome duplication, the two blocks need to be paralogous, here they are not. Note syntenic context for the primate retroposed gene DHFRL1 has, as expected, no relation to either site: ARL13B DHFRL1 NSUN3.)
Thus the duplication event occurred in the last common ancestor of amphibians and ray-finned fish -- the stem bony vertebrate thus had both DHFR and DHFRL2 at these two sites in the ancestral chromosomes. The two genes have persisted some 450 myr to contemporary amphibians, coelocanth and ray-finned fish but the DHFRL2 gene was lost in the amniote stem with unknown consequences.
The gene duplication may be slightly older but assemblies are inadequate in chondrichthyes, lamprey and cephalochordate. The tunicate Ciona has a close anti-parallel tandem duplication of DHFR in an altogether novel syntenic location (tunicate gene order is typically scrambled).
A paralog of DHFR in coelocanth cannot be confirmed as DHFRL2 by synteny because the contigs are too small. However it clusters as expected in the combined gene tree. As expected for a 'living fossil', both DHFR and DHFRL2 have evolved slower than other species. Thus on Blastp query against human, both proteins 'jump the queue' (score higher than later diverging species). However this could be due to more rapid evolution in the other species.
The alignment below compares separately determined conservation in species-matched sets of 15 DHFRL2 and DHFR sequences. While DHFR is significantly more conserved, DHFRL2 also has very significant conservation (shown below at the 90% level). Although certain residues are conserved between both, each of the proteins has a considerable number of conserved residues not shared.
DHFRL2_xenTro KPVKLIAAACNSMGIGLNGYLPWN---LPNEFKYLLDTITRVTQPGKKNLLLWGRQSFETFDESLLPLPNCIIALLSRKLSTVPPYVSYLCKNEEEILKFASTPPLNDEIETIWVLGGVESYKNLMQHPWCNHIYFTKIMADFECDTFFPEFDKNIFKLKENFPGVPSGIQEENGVKYVFQVYQR DHFRL2_ambTig KPIKIIVAAGRNRGIGIKGNLPWN---LPNEFKYFLDKITSVSNPGKKNFIMVGRTSFEGFPDCVLPLPNTIVAILSKTLRSTPKHAAYVCQDVQEVLDLASSPPLSKDIETIWALGGVQSYMEVMHHPWCNQIFFTNVMADFECDTFFPAIDTGVFNLVEQFPGVPLGLQEENGIKYEFQVYER DHFRL2_latCha KPICLIVAACKHMGIGKGGDLPWH---LPNEFKHFLKMTTTVSVPGKKNLLVSGRKSYEPFPKSCMPLPNCYIAVLSRTLKSVPKHAHYLCRDLPSAIKLASTPPLSKEIETIWIIGGVETYIEA-KHPWCELLYLTEIMAEFDCDTFFPEFDRKVYKLVDEFPGVPTGIQEEEGIKYKFHVFKR DHFRL2_danRer KPIRLIAAACRDMGIGKDGQIPWC---LPKEFQFLLDTITAVSAPGKKNLIVWGRICWFSCPETVFPLANCINLVLSRKMISVPPHAHYLCKDFDSIIRLVSEPPLCHTVEVIWILGGTEVYKESLEHPWCDLIYLTDIMANFECDVFFPEFDPNIFRKQKSFPGVPDEIIEENGIKFQFQVFK- DHFRL2_pimPro KPIRLIAAACRDMGIGKNGQLPWS---LPKEFQFFLDTITAVSTPGKKNLVVWGRVSWFSCPETVFPLANCFNLVLSRKLNANPPRAHYLCKDFESVIRLVSEPPLCHIVETIWVLGGPEVYKESLEHPWCDRIYLTDIMADFDCDVFFPKFDCNIFRKQKGFPGVPDEIHEENGIKFQFQVFKR DHFRL2_ictPun KPIRLVAAACRNMGIGKDGRLPWN---LPTEFQFFLNTITAVCKSGKKNLIIWGKYSWFSCPESVFPLANSLHVVLSKKLMSVPKRAHYVCEDFVSAVKLASHPPLNDLIETIWILGGTQVYKEALEHPWCDLIYLTDIMADFDCNVFFPSFDQKFYRKQNKFPGVPNEIQEENGIKFRFQVFMK DHFRL2_tetNig KPVRLIAAKCNGGGIGKDNRMPWS---LPSEFRFFLSTITRVSRPGKMNMIIWGKQCWISHPESTFPLPNVLHTVLSRTLFAVPDHAHFVCKTLDAAVRLASQPPLADLIEVVWIVGGVQVYKEAMEHPWCDLIYLTDIMAEFECDVFFPEFDRQLFQVQDGFPDVPDGIQEENGIKYKCQVYKR DHFRL2_oncMyk KPVRLIAAACNNMGIGKDGHLPWD---LPTEFKFFLETTTNVSLPGKFNMMVWGRGCWFSNPDSFFSLPNVLHVVLSTTLSTLPEHAHFLCQDFDSMISLALLQPLCDLVETIWVVGGPRVYQEALMHPWCELVFLTDIMADFDCDVFFPQFDRSVFKKQQRFPGVPNEMQEENGVTFKYEVFKR DHFRL2_takRub KPVRLIAAKCNGGGIGKDNRMPWS---LPAEFQYFLNTVTRVSRPGNMNMMVWGKQCWVSHPDSTFPLPNILHAVLSKTLLTVPDHAHFLCESLDAAVRLASEPPLADLIEIIWIVGGVQVYKEAMEHPRCDLIYLTDIMAEFECDVFFPEFDKKLFEVQDRFPDVPNGIQEDNGIKYKCQVYKR DHFRL2_oreNil KPVRLIAAACNDMGIGKDGKLPWD---LPTEFQYFVNTVKTVSRPGRMNFMVWGRLCWDSHPESLFPLPNILHVVLSKTLDTVPDHAHFLCRDFDSAIRLAAEPPLADLIETIWIVGGVQVYKDALNHPWCDLLYLTDVMADFDCDVFFPEFDKNLFQLQEGFPGVPSEIQEENGIKFKYQVFKR DHFRL2_hipHip KPVRLIAAACNDMGIGKDGKLPWK---LPSEFQYFLNTVTRVSRPGKMNMMVWGKLCWFSHPETMFPLANTLHVVLSTKLNKVPDHAHFLSEDFESAVRMSAEPPLADLIENIWVVGGMQVYKDALSHPWCDLVYLTNVMADFECDVFFPEFDRGVFKTQKDFLEVPSGIQEEDGIKYKFQVFKK DHFRL2_poeRet KPVRLIAANCNNMGMGKDGTMLWS---LPSEFQYFMNTITRVSRPGNMNLLIWGRLCWNSHPENIFPLANTLHAVLSKTSSSVPDHAHFLCQDFESAVRLAAQPPLSGIIETVWILGGTQVYEDALKHPWCDLLYLTDVMADFDCDVFFPDFDRELFKLQEAFPDVPSEVQEENGIRFKCQVFKK DHFRL2_gasAcu KPVRLIAAACSGGGIGKDGQMPWD---LPSEFRYFRNHVQGVSRPGKMNMMVWGKLCWYSVPKPDFPLPNVLHVVLSKTLESVPDHAHFLCEDLDAAARLAVQPPLADLIETIWVVGGTQVYEVALKHPWCDLVYLTDVMADFDCNVFFPEFDRGLFKVQEGFPGVPSEIQEENGIKYKFQVFKK DHFRL2_dicLab KPIRLIAVVCNGNGIAKDGKMPWN---LPSEFQFFLKTVTTVSRPGKMNFLVWGKRTWQSHPETTLPLANTLHVVLSTELKTVPDHAHFLSQDFESAVRLASQPPLADLIETIWIIGGTQVYEDALKHPWCDLVYLTDVMADFECNVFFPEFDRGLFKLLENFPGVPSGIQEENGTKYKCQVFKR DHFRL2_gadMor KPVRLIAAACNDLGIGKNGKLPWS---LPNEFRYYLNNLSRVSRPGMFNLVVWGRLNWFSQEE--FLKENVILVVLSKTLGVVPDNAQYLREDLEGAIRLAASPPLSDIIETIWVVGGTGVYKESLMHPWCDLIYLTDVMANIDCDVFFPNFDRQIFKKQDRFPGVPSEIQEENGVKYKYEVYKK DHFR_homSap GSLNCIVAVSQNMGIGKNGDLPWPPLR--NEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEK DHFR_xenTro PFLHAVVAVCPNQGIGKEGSLPWPLLR--NEFKHFQRLTMTPTVEDKKNVVIMGRKTWFSIPEKNRPLKERINIVLSKELKEPPTGAHYLSKSLAEAIDLLEDPELKDKVDLVWVIGGSSLYQELMEKPVNQRWFVTRILQEFECDTYLPEIDLNSFRLLPEYPGISPELQEENGVQYKFEVYEK DHFR_ambMex RILNAIVAVCPSMGIGKDGNLPWPILR--NEFKYFQRMTMTATQEGKQNVVIMGRKTWLSIPEKNRPLKGRINIVLSRELTAPPPGAHYLANSLDGALELLETRELAGKVDLLWIIGGSSLYRELIGRPHHQRLFVTRILKEFECDTFLPEFDLEKYRLLPEYPGVSLGIQEENGIQYKFEVYEK DHFR_latCha RLLNSIVAVCPNLGIGKDGNLPWHPKRLSNEFRYFQKMTTTPTVEGKQNVVIMGRKTWFSIPEKNRPLKGRVNVVLSRELQEAPEGAHYLARDLDSALKLLESPELADRVDLVWIIGGSALYKEALEHPSQHRLFVTRVLQQFECDTYLPEINFNKFTLLPEFPGVSSAIQEESGIQYKFEVYEK DHFR_danRer RILNCIVAVCPDMGIGKNGNLPWHPIRLSNELKHFQKMTMTPSDEGKKNVVIMGRKTWFSIPAAHRPLKNRINIVLSRELKTAPEGAHYLASDFSSALHLLDSGELEKLVDQVWIIGGSSLYKEVMERSGHRRLFVTRILKQFDCDTFIPNFDMDKYKLLPEFPGVPVGLQEDNGVQYLFEVYES DHFR_cypCar RILNSIVAVCPDMGIGKNGNLPWHPIRLSNEFKHFQKMTMTPLVEGKKNVVLMGRKTWFSIPAANRPLKNRINIVLSRELKTAPEGAHYLASDFSSALHLLDSSELEKQVDQVWIIGGSALYKEVMESSGPRRLFVTRVLKQFDCDTFIPDINMDKYKLLPEFPGVPTGLQEENGVQYVFEVYES DHFR_ictPun RVLNCIVAVCPDMGIGRNGNLPWHPIRLSKEFKHFQKMTMTPTVEGKKNVVIMGRKTWFSIPAQNRPLKNRINVVLSRELKTAPEGAHYLAADFSSALRILENAELEAQADQVWIIGGSSLYKEVMESSAHQRLFVTRILKQFDCDTFIPNIDLDKFRLLPEFPGVPAGLQEENGLQYVFEVYES DHFR_oncMyk RVLNCIVAVCPDMGIGNNGNLPWHPKRLNNEFKYFQKMTMTSSVEGKQNAVIMGRKTWFSIPERNRPLKNRINIVLSRELKEPPAGAHQLASDFRSALRLLDTAEMAERVDQVWVIGGSSLYEEMMESQGTRRLFVTRILQQFKSDTFLPEINQDKYRLLPEFPGVPQDLQEEAGLQYRFEVYES DHFR_esoLuc RVLNCIVAVCPNMGIGNKGNLPWHPKRLNNEFKYFQKMTMTPFVEGKQNVDIMGRKTWFSIPERNRPLKNRINIVLSRELKEPPPGAHHLASDFSSALRLLDTAEMAERVDQVWVIGGSSLYKEMMESEGIRRLFVTRILQQFESDTFLPEINQDKYRLLPEFPGVPQDVQEESGIQYRFEVYES DHFR_gadMor RVLNCIVAVCPDAGIGYKGDLPWHPTRLNNEFKHFRRLTVTPGAEDKQNVVIMGRKTWNSIPEKNRPLNDRINIVLSRQLEAPPSGAHHLARDLPSALRLLDV-PEGLLADQVWLIGGSSMYSEVMETPCPRRLFVTRILKQFPSDTFFPDISPAQFRLLPEYPGVSSELQEENGIQYRFQVYQS DHFR_tetNig RVLNAIVAVCPDLGIGRNGDLPWHPIRLDNEFKHFRKMTSTPSVNGKQNVVIMGRKTWFSIPEKHRPLANRINIVLSRRSREPPAGAHHLAHDLSSALQLVET-QLAEQADQVWVIGGSSLYQEMMERPGTSRLFVTHVLKQFDCDTFLPEIRPQRYRLLPQFPGVPQELQEEKGIQYRYQVYES DHFR_oreNil RVLNAIVAVCPDRGIGNKGNLPWHPIRLSKEFAHFRKMTATPSVKGKQNVVIMGKKTWYSIPEKNRPLSNRINIVLSRECKVPPAGAHYLASDFSSALRLIDT-ELADQADQVWVIGGSSLYKEMMGSTGMRRLFVTQILKQFECDTFLPEISLDKYRLLPEFPDVPQELQEENGIQYRFKVYES DHFR_hipHip RILNGIVAVCPDLGIGNRGNLPWHPVRLSNEFKHFRSMTATPSEKGKQNVVIMGRKTWFSIPEKNRPLNNRINIVLSRQCKAPPAGAHHLSADFSSALRLVDT-ELADQADQVWVIGGSSLYKEMMESPGTRRLFVTRILKQFDCDTFLPEISPDKYRQLPEFPGVPQELREENGIQYRFEVYES DHFR_gasAcu RVLNGIVAVCPDLGIGCHGNLPWHPLRLSNEFKHFRRMTATASVKDKQNVVIMGRKTWFSIPEKNRPLNNRINIVLSRELKAPPAGAHHLAPDFSSALRLVDT-ELAEQADQVWVIGGSSLYKELLESPGTRRLFVTRILKQFECDTFLPEICPDRYRLLPEFPGVPEELQEENGVQYRFEVYES DHFR_dicLab RILNGIVAVCPDLGIGMNGNLPWHPVRLGNEFKHFRTMTATPSVKGKQNVVIMGRKTWFSIPEKNRPLNNRINIVLSKECKVPPAGAHHLAADFNSALRLVET-ELADQADQVWVIGGSSLYKELMESPGTRRLFVTRILKQFECDTFLPEICPDKYQLLPEFPGVSQELQEENGIQYRFEVYES DHFR_spaAur RIVNGIVAVCPDLGIGNNGNLPWHPVRLNREFKHFRTMTATPSVKGKQNVVIMGRKTWFSIPDKFRPLNNRINIVLSRECKVPPAGAHHLAADFSSALSLVDT-ELADQADQVWVIGGSSLYKELMESTRTSRLFVTRILKQFECDTFLPEISPDKYSLLPEFPGVPQGLQEENGIQYKFEVYER DHFRL2_latCha KPICLIVAACKHMGIGKGGDLPWH---LPNEFKHFLKMTTTVSVPGKKNLLVSGRKSYEPFPKSCMPLPNCYIAVLSRTLKSVPKHAHYLCRDLPSAIKLASTPPLSKEIETIWIIGGVETYIEA-KHPWCELLYLTEIMAEFDCDTFFPEFDRKVYKLVDEFPGVPTGIQEEEGIKYKFHVFKR 90% conserv KP..LI.A.C...GIG.....PW.---LP.EF.........V..PG..N....G.............L.N.....LS......P..A................PPL....E..W..GG...Y.....HPWC.....T..MA.F.C..FFP.FD.........FP.VP....EE.G......V... unique cons KP..L...................---LP............V..PG.......................N.................................PpL....e................HPWC........mA...C..FF..FD.........F..V................... shared cons .....I.A.C...GIG.....PW.---...EF................N....G.............L.......LS......P..A.................2.....2..W..GG...Y.................2.........P.............P.V.....EE.G......V... unique cons ..LN..V.V.P.......G.L.....R.......F...T.T.....K...VIM..KTW.SIP...RP...RIN.V..........G.H.L......AL.L....e.....d.V..I..S....E........RLFV...L.....DT...........LLPE..G.....Q.....QY.F..YE. 90% conserv ..LN.IVAVCP..GIG..G.LPW...R...EF..F...T.T.....K.N.VIMG.KTW.SIP...RPL..RIN.VLS......P.GAH.L......AL.L....E.....D.VW.IGGS..Y.E........RLFVT..L..F..DT..P........LLPE.PGV....QEE.G.QY.F.VYE. DHFR_homSap GSLNCIVAVSQNMGIGKNGDLPWPPLR--NEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEK >DHFRL2_xenTro Xenopus tropicalis (frog) BTBD11 RASGRF2 MSH3 DHFRL2 CRY1 MTERFD3 SIRT2 RIC8A RFX4 on truncated contig chr GL173571, dozens of transcripts MREAPNGTKPVKLIAAACNSMGIGLNGYLPWNLPNEFKYLLDTITRVTQPGKKNLLLWGRQSFETFDESLLPLPNCIIALLSRKLSTVPPYVSYLCKNEEEILKFASTPPLNDEIETIWVLGGVESYKNLMQHPWCNHIYFTKIMADFECDTFFPEFDKNIFKLKENFPGVPSGIQEENGVKYVFQVYQRDLLVDLL* >DHFRL2_ambTig Ambystoma tigrinum (salamander) CN067598 no synteny available MSEARERNGPKPIKIIVAAGRNRGIGIKGNLPWNLPNEFKYFLDKITSVSNPGKKNFIMVGRTSFEGFPDCVLPLPNTIVAILSKTLRSTPKHAAYVCQDVQEVLDLASSPPLSKDIETIWALGGVQSYMEVMHHPWCNQIFFTNVMADFECDTFFPAIDTGVFNLVEQFPGVPLGLQEENGIKYEFQVYERKGSISM* >DHFRL2_latCha Latimeria chalumnae (coelocanth) AFYH01018047 AFYH01018044 AFYH01018043 AFYH01018041 second exon missing, filled by consensus MGIGKKPICLIVAACKHMGIGKGGDLPWHLPnefkhflkmtttvsvpGKKNLLVSGRKSYEPFPKSCMPLPNCYIAVLSRTLKSVPKHAHYLCRDLPSAIKLASTPPLSKEIETIWIIGGVETYIEAKHPWCELLYLTEIMAEFDCDTFFPEFDRKVYKLVDEFPGVPTGIQEEEGIKYKFHVFKRD >DHFRL2_danRer Danio rerio BC165261 BTBD11 MSH3 CRY1 DHFRL2 MTERFD3 SIRT2 RIC8A RFX4 MNIEGEEMRKPIRLIAAACRDMGIGKDGQIPWCLPKEFQFLLDTITAVSAPGKKNLIVWGRICWFSCPETVFPLANCINLVLSRKMISVPPHAHYLCKDFDSIIRLVSEPPLCHTVEVIWILGGTEVYKESLEHPWCDLIYLTDIMANFECDVFFPEFDPNIFRKQKSFPGVPDEIIEENGIKFQFQVFKKIKN* >DHFRL2_pimPro Pimephales promelas (fathead minnow) DT166997 MNIEGEEMRKPIRLIAAACRDMGIGKNGQLPWSLPKEFQFFLDTITAVSTPGKKNLVVWGRVSWFSCPETVFPLANCFNLVLSRKLNANPPRAHYLCKDFESVIRLVSEPPLCHIVETIWVLGGPEVYKESLEHPWCDRIYLTDIMADFDCDVFFPKFDCNIFRKQKGFPGVPDEIHEENGIKFQFQVFKrA* >DHFRL2_ictPun Ictalurus punctatus (catfish) FD330522 MKPRNEVQKPIRLVAAACRNMGIGKDGRLPWNLPTEFQFFLNTITAVCKSGKKNLIIWGKYSWFSCPESVFPLANSLHVVLSKKLMSVPKRAHYVCEDFVSAVKLASHPPLNDLIETIWILGGTQVYKEALEHPWCDLIYLTDIMADFDCNVFFPSFDQKFYRKQNKFPGVPNEIQEENGIKFRFQVFMKES >DHFRL2_tetNig Tetraodon nigroviridis (pufferfish) CR727807 cDNA MEPAKVIKKPVRLIAAKCNGGGIGKDNRMPWSLPSEFRFFLSTITRVSRPGKMNMIIWGKQCWISHPESTFPLPNVLHTVLSRTLFAVPDHAHFVCKTLDAAVRLASQPPLADLIEVVWIVGGVQVYKEAMEHPWCDLIYLTDIMAEFECDVFFPEFDRQLFQVQDGFPDVPDGIQEENGIKYKCQVYKRERAAEDQQ* >DHFRL2_oncMyk Oncorhynchus mykiss (rainbow trout) CX259371 fragment qkkpvrlIAAACNNMGIGKDGHLPWDLPTEFKFFLETTTNVSLPGKFNMMVWGRGCWFSNPDSFFSmLPNVLHVVLSTTLSTLPEHAHFLCQDFDSMISLALLQPLCDLVETIWVVGGPRVYQEALMHPWCELVFLTDIMADFDCDVFFPQFDRSVFKKQQRFPGVPNEMQEENGVTFKYEVFKREICSAEVI* >DHFRL2_takRub Takifugu rubripes (fugu) CAAB02000170 MEPAKVIKKPVRLIAAKCNGGGIGKDNRMPWSLPAEFQYFLNTVTRVSRPGNMNMMVWGKQCWVSHPDSTFPLPNILHAVLSKTLLTVPDHAHFLCESLDAAVRLASEPPLADLIEIIWIVGGVQVYKEAMEHPRCDLIYLTDIMAEFECDVFFPEFDKKLFEVQDRFPDVPNGIQEDNGIKYKCQVYKRKTAGKDQQ* >DHFRL2_oreNil Oreochromis niloticus (tilapia) XM_003447073 dihydrofolate reductase-like METSWEKLQKKPVRLIAAACNDMGIGKDGKLPWDLPTEFQYFVNTVKTVSRPGRMNFMVWGRLCWDSHPESLFPLPNILHVVLSKTLDTVPDHAHFLCRDFDSAIRLAAEPPLADLIETIWIVGGVQVYKDALNHPWCDLLYLTDVMADFDCDVFFPEFDKNLFQLQEGFPGVPSEIQEENGIKFKYQVFKRDTADTV* >DHFRL2_hipHip Hippoglossus hippoglossus (Atlantic halibut) EB034565 frameshift fixed MEKSREKRQKKPVRLIAAACNDMGIGKDGKLPWKLPSEFQYFLNTVTRVSRPGKMNMMVWGKLCWFSHPETMFPLANTLHVVLSTKLNKVPDHAHFLSEDFESAVRMSAEPPLADLIENIWVVGGMQVYKDALSHPWCDLVYLTNVMADFECDVFFPEFDRGVFKTQKDFLEVPSGIQEEDGIKYKFQVFKKSICDAI* >DHFRL2_poeRet Poecilia reticulata (guppy) ES386580 MEKDLEKVQKKPVRLIAANCNNMGMGKDGTMLWSLPSEFQYFMNTITRVSRPGNMNLLIWGRLCWNSHPENIFPLANTLHAVLSKTSSSVPDHAHFLCQDFESAVRLAAQPPLSGIIETVWILGGTQVYEDALKHPWCDLLYLTDVMADFDCDVFFPDFDRELFKLQEAFPDVPSEVQEENGIRFKCQVFKKKTDDTM >DHFRL2_gasAcu Gasterosteus aculeatus (stickleback) ENSGACT00000017858 MSGDNVQKKPVRLIAAACSGGGIGKDGQMPWDLPSEFRYFRNHVQGVSRPGKMNMMVWGKLCWYSVPKPDFPLPNVLHVVLSKTLESVPDHAHFLCEDLDAAARLAVQPPLADLIETIWVVGGTQVYEVALKHPWCDLVYLTDVMADFDCNVFFPEFDRGLFKVQEGFPGVPSEIQEENGIKYKFQVFKKETGDAV >DHFRL2_dicLab Dicentrarchus labrax (seabass) FM022412 METVQRKPIRLIAVVCNGNGIAKDGKMPWNLPSEFQFFLKTVTTVSRPGKMNFLVWGKRTWQSHPETTLPLANTLHVVLSTELKTVPDHAHFLSQDFESAVRLASQPPLADLIETIWIIGGTQVYEDALKHPWCDLVYLTDVMADFECNVFFPEFDRGLFKLLENFPGVPSGIQEENGTKYKCQVFKRETGDTV >DHFRL2_gadMor Gadus morhua (Atlantic cod) ES776457 MSLQKKPVRLIAAACNDLGIGKNGKLPWSLPNEFRYYLNNLSRVSRPGMFNLVVWGRLNWFSQEEFL KENVILVVLSKTLGVVPDNAQYLREDLEGAIRLAASPPLSDIIETIWVVGGTGVYKESLMHPWCDLIYLTDVMANIDCDVFFPNFDRQIFKKQDRFPGVPSEIQEENGVKYKYEVYKKDIHHQDKLM* Blastp scores relative to human DHFR DHFR_latCha Latimeria chalumnae (coelocanth) AFYH011508... 709 1.9e-74 DHFR_xenTro Xenopus tropicalis (frog) CT025223 RASGFRA... 653 1.5e-68 DHFR_ictPun Ictalurus punctatus (catfish) FD330522 613 2.6e-64 DHFR_danRer Danio rerio (zebrafish) NM_131775 CELA2A BC... 607 1.1e-63 DHFR_gasAcu Gasterosteus aculeatus (stickleback) ENSGAC... 600 6.3e-63 DHFR_tetNig Tetraodon nigroviridis (pufferfish) CR710339 596 1.7e-62 DHFR_oreNil Oreochromis niloticus (tilapia) GR620119 583 4.0e-61 DHFRL2_latCha Latimeria chalumnae (coelocanth) AFYH010180... 453 1.4e-47 DHFRL2_oreNil Oreochromis niloticus XM_003447073 dihydrof... 418 7.1e-44 DHFRL2_danRer Danio rerio BC165261 BTBD11 MSH3 CRY1 DHFR... 403 4.7e-42 Identity: 75/181 (41%) Similar: 115/181 (63%) DHFRL2_gasAcu Gasterosteus aculeatus (stickleback) ENSGAC... 414 3.2e-43 DHFRL2_ictPun Ictalurus punctatus (catfish) FD330522 406 2.3e-42 DHFRL2_tetNig Tetraodon nigroviridis (pufferfish) CR72780... 404 3.7e-42 DHFRL2_xenTro Xenopus tropicalis (frog) BTBD11 RASGRF2 M... 366 3.9e-38 Identity: 72/179 (40%) Similar: 108/179 (60%) Supporting syntenic data: Brown: definite DHFRL2; Green: no data but DHFRL2 expected; Red: DHFRL2 lost Gnathostomata (jawed vertebrates) Chondrichthyes (cartilaginous fish) Teleostomi (bony vertebrates) Actinopterygii (ray-finned fishes) Actinopteri Chondrostei (sturgeons) Neopterygii Polypteriformes Polypteridae (bichirs) Sarcopterygii Coelacanthiformes (lobe-finned fishes) Dipnoi (lungfishes) Tetrapoda (tetrapods) Amniota (amniotes) Amphibia (amphibians) Gene order in Homo sapiens (human): FAM151B 800 9 276 276 100.0% 5 ++ 79797610 79837648 40039 ANKRD34B 1542 1 514 514 100.0% 5 +- 79854297 79855838 1542 DHFR 556 1 187 187 100.0% 5 +- 79924909 79950308 25400 MSH3 3388 1 1137 1137 100.0% 5 ++ 79950547 80171678 221132 RASGRF2 3675 1 1237 1237 99.9% 5 ++ 80256558 80521586 265029 RFX4 2203 1 744 744 99.9% 12 ++ 106995055 107155244 160190 RIC8B 1552 1 520 520 100.0% 12 ++ 107168524 107279790 111267 [SIRT3] C12orf23 347 1 116 116 100.0% 12 ++ 107360895 107365166 4272 [CHST1] MTERFD3 1155 1 385 385 100.0% 12 +- 107371338 107372492 1155 [DHFRL2] CRY1 1747 1 586 586 100.0% 12 +- 107386568 107486739 100172 BTBD11 3296 1 1104 1104 100.0% 12 ++ 107712718 108051492 338775 Gene order in Taeniopygia guttata (finch): RASGRF2 1679 1 1237 1237 95.2% Z +- 72041141 72156983 115843 MSH3 1131 200 1101 1137 82.0% Z +- 72194865 72285577 90713 DHFR_ 319 1 187 190 90.5% Z ++ 72298623 72310674 12052 ANKRD34B 258 10 183 514 74.8% Z ++ 72330255 72330776 522 RASGRF2 739 541 896 1237 85.7% Z ++ 72356949 72372598 15650 BTBD11_xenTro 1894 108 997 997 90.1% 1A +- 53269530 53431966 162437 CRY1_xenTro 1260 46 574 577 95.3% 1A +- 53490624 53504662 14039 MTERFD3_xenTro 146 132 368 369 63.1% 1A ++ 53539437 53540147 711 C12orf23_homSa 225 20 116 116 88.7% 1A +- 53545152 53545442 291 RIC8B_xenTro 693 1 404 414 83.1% 1A +- 53588230 53608076 19847 RFX4_xenTro 1422 29 691 691 91.2% 1A +- 53628722 53681344 52623 BTBD11_xenTro 164 445 840 997 74.9% 5 +- 6143735 6154710 10976 SIRT3_xenTro 198 131 311 401 73.9% 5 ++ 7212746 7213932 1187 RIC8B_xenTro 207 15 380 414 72.0% 5 +- 7219279 7223031 3753 CRY1_xenTro 793 53 488 577 88.9% 5 +- 22665497 22682488 16992 CHST2_xenTro 700 1 406 407 80.0% 5 ++ 22765821 22767074 1254 C12orf23_homSa 138 25 116 116 75.0% 5 +- 22774918 22775193 276 Gene order in Anolis carolinensis (lizard): FAM151B 154 72 171 276 81.4% 2 ++ 17132292 17134230 1939 DHFR_galGal 218 1 153 190 87.9% 2 +- 17184692 17203894 19203 MSH3 1054 202 1094 1137 82.3% 2 ++ 17214220 17270142 55923 RASGRF2 2613 98 1237 1237 93.1% 2 ++ 17343088 17434824 91737 RFX4_xenTro 1453 1 691 691 91.0% 5 ++ 19285834 19332634 46801 RIC8B_xenTro 610 2 380 414 80.2% 5 ++ 19352614 19369230 16617 C12orf23_homSa 210 25 116 116 88.1% 5 ++ 19412228 19412503 276 MTERFD3_xenTro 77 133 320 369 57.6% 5 +- 19415576 19416139 564 CRY1_xenTro 1409 1 575 577 96.3% 5 ++ 19436254 19471304 35051 BTBD11_xenTro 1677 1 997 997 89.7% 5 ++ 19529954 19797513 267560 Gene order in Xenopus tropicalis (frog): FAM151B_xenTro 729 1 257 260 99.4% GL172749 +- 48220 68819 20600 ANKRD34B_xenTro 1457 1 501 501 99.8% GL172749 ++ 37857 39410 1554 DHFR_xenTro 562 1 189 189 100.0% GL173611 +- 10223 18119 7897 MSH3_xenTro 2477 1 847 847 99.7% GL173611 ++ 25550 81243 55694 RASGRF2_xenTro 3396 1 1195 1195 99.8% GL173611 ++ 107705 231228 123524 RFX4_xenTro 1766 70 691 691 99.7% GL174569 +- 5720 25164 19445 RIC8B_xenTro 1175 1 401 414 100.0% GL173571 +- 232468 255175 22708 SIRT3_xenTro 1167 10 401 401 100.0% GL173571 ++ 223930 227352 3423 C12orf23_xenTro 66 1 22 114 100.0% GL173571 +- 191964 192029 66 CHST1_xenTro 1171 1 405 405 99.7% GL173571 ++ 175980 184029 8050 MTERFD3_xenTro 1055 12 369 369 99.2% GL173571 +- 69636 70712 1077 DHFRL2_xenTro 254 35 197 384 99.3% GL173571 ++ 7955 10720 2766 CRY1_xenTro 1627 1 576 577 100.0% GL173033 +- 9078 44385 35308 BTBD11_xenTro 2800 1 997 997 99.8% GL173033 ++ 181463 298215 116753 Gene order in Danio rerio (zebrafish): ANKRD34B_danRer 960 1 453 453 98.7% 10 +- 3058846 3060435 1590 FAM151B_danRer 504 1 169 169 100.0% 5 +- 27809804 27813417 3614 ANKRD34B_danRer 284 1 162 453 81.6% 5 ++ 27800655 27803336 2682 DHFR_danRer 553 1 190 190 99.0% 5 ++ 27782945 27789956 7012 MSH3_danRer 1381 1 472 472 100.0% 5 ++ 52945585 53286850 341266 RASGRF2_danRer 3257 1 1191 1191 99.3% 5 ++ 53315572 53434601 119030 RFX4_danRer 2036 1 715 715 99.7% 18 +- 14920893 14956527 35635 RIC8B_danRer 947 1 363 363 99.1% 18 +- 14895538 14907434 11897 SIRT3_xenTro 272 128 356 401 74.6% 18 ++ 14871213 14875920 4708 C12orf23_danRer 328 1 112 116 99.2% 18 +- 14849452 14851702 2251 CHST1_xenTro 259 57 330 405 67.8% 18 ++ 14773066 14773905 840 MTERFD3_danRer 375 28 360 363 70.3% 18 +- 14735350 14736351 1002 DHFRL2_danRer 546 1 187 187 99.3% 18 ++ 14718538 14725012 6475 CRY1_danRer 1192 1 498 520 90.2% 18 ++ 14692957 14710707 17751 BTBD11_danRer 2081 7 1008 1008 92.2% 18 +- 14542238 14687242 145005